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Table 2 CTF Overall sensitivity and accuracy

From: Error analysis in the determination of the electron microscopical contrast transfer function parameters from experimental power Spectra

Parameter

Symbol

Overall Sensitivity

Accuracy (%)

Microscope voltage

V

207.59

NA

Major defocus

Δf M

154.19

1.18

Minor defocus

Δf m

153.13

1.07

Background PSD

s m

100.00

5.50

Background PSD

s M

94.34

4.38

Background PSD

b

50.04

5.26

Background PSD

K G

49.92

11.91

Fraction of scattered electrons

Q 0

13.28

21.86

Chromatic aberration

C a

11.26

14.29

Energy spread

ΔV/V

9.87

52.95

Defocus azimuthal angle

θ

4.44

NA

CTF Gain

K

3.14

7.41

Aperture semiangle

α

1.53

54.23

Spherical aberration

C s

1.33

NA

Background PSD

K s

0.76

14.70

Background PSD

C m

0.51

40.61

Background PSD

K g

0.45

11.22

Focal plane displacement

ΔR

0.43

NA

Background PSD

G M

0.43

23.58

Background PSD

C M

0.39

38.18

Background PSD

G m

0.37

29.72

Sampling rate

T m

0.34

NA

Background PSD

c m

0.27

40.60

Background PSD

θ G

0.27

NA

Background PSD

g M

0.20

17.51

Background PSD

g m

0.16

13.29

Background PSD

c M

0.08

19.07

Perpendicular displacement

ΔF

0.05

NA

Background PSD

θ s

0.04

NA

Background PSD

θ g

0.02

NA

  1. The overall sensitivity column is the sum for each parameter of the percentages shown in Table 1. The accuracy column shows the accuracy in the estimation of each parameter as estimated by bootstrapping (see text).