Low-resolution structural studies of human Stanniocalcin-1

BMC Structural Biology

Table 1 Prediction of putative post-translational modification sites in human STC1.

Residue	Modification	Buried/Exposed Residue[51]	Predictor (Score)	Ref.
N₆₂	N-glycosylation	nd	NetGlyc (0.61)	[17, 39, 41, 61]
K₈₃	Sumoylation	e	SUMOplot™ (0.79)	$
S₉₅	PKC* phosphorylation	b	NetPhos (0.844)/NetPhosK (0.630)	[50]
S₁₁₅	PKC* phosphorylation	nd	NetPhos (0.788)/NetPhosK (0.722)	[50]
	PKA* phosphorylation	nd	NetPhos (0.788)/NetPhosK (0.841)	[50]
	RSK* phosphorylation	nd	NetPhos (0.788)/NetPhosK (0.601)	nd
Y₁₅₉	INSR* phosphorylation	nd	NetPhos (0.929)/NetPhosK (0.539)	nd
S₁₇₆	PKC* phosphorylation	e	NetPhos (0.938)/NetPhosK (0.630)	[50]
T₁₇₇	PKC* phosphorylation	nd	NetPhos (0.983)/NetPhosK (0.640)	[50]
S₁₈₁	PKA* phosphorylation	nd	NetPhos (0.993)/NetPhosK (0.647)	[50]
T₂₀₅	PKC* phosphorylation	nd	NetPhos (0.606)/NetPhosK (0.815)	[50]
	Cdc2* phosphorylation	nd	NetPhos (0.606)/NetPhosK (0.509)	[50]
T₂₁₆	PKG* phosphorylation	e	NetPhos (0.817)/NetPhosK (0.600)	[50]
S₂₃₅	GSK3* phosphorylation	nd	NetPhos (0.986)/NetPhosK (0.508)	[50]
	Cdk5* phosphorylation	nd	NetPhos (0.986)/NetPhosK (0.551)	[50]
S₂₃₇	PKC* phosphorylation	nd	NetPhos (0.531)/NetPhosK (0.647)	[50]
T₂₄₂	PKG* phosphorylation	nd	NetPhos (0.523)/NetPhosK (0.693)	[50]

High score predictions of glycosylation, sumoylation and phosphorylation on STC1 sequence are presented. Predicted modifications within the pro-peptide region were excluded. Putative phosphorylated residues shown here are only those that were both predicted with the highest scores by the NetPhos server and additionally were predicted by NetPhosK, which suggests a specific kinase for the same site. References are related to additional experimental support for the predicted modification, if available. * indicate kinase as predicted by NetPhosK [protein kinase A C or G (PKA; PKC and PKG); 90-kDa Ribosomal S6 Kinase (pp90RSK or RSK); Insulin receptor (INSR); cell division cycle 2 (cdc2 or p34 protein kinase); ciclin dependent kinase 5 (cdk5); Glycogen synthase kinase 3 (GSK3)]; e = exposed residue, b = buried residue, nd = not determined; $ = unpublished data.

ISSN: 1472-6807