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Table 5 The effects of cooling and reactivation on the degree of dissociation of apo wt Trpase from E. coli and P. vulgaris

From: Conformational changes and loose packing promote E. coli Tryptophanase cold lability

Sample

T (°C)

Degree of dissociation (%)

  

Tetramer

dimmer

monomer

P. vulgaris Trpase

25°C

37.3 ± 1.2

44.5 ± 3.4

18.2 ± 4.0

P. vulgaris Trpase

2°C, 24 h

21.7 ± 0.6

45.4 ± 0.7

32.9 ± 1.6

P. vulgaris Trpase +1 mM PLP

25°C, 2 h

92.6 ± 0.8

7.4 ± 0.2

-

E. coli Trpase

25°C

30.0 ± 2.2

70.0 ± 5.0

-

E. coli Trpase + 1 mM PLP

25°C, 1 h

100 ± 0.6

-

-

  1. Data in parenthesis correspond to the high resolution shell.
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BMC Structural Biology

ISSN: 1472-6807

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