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Table 5 The effects of cooling and reactivation on the degree of dissociation of apo wt Trpase from E. coli and P. vulgaris

From: Conformational changes and loose packing promote E. coli Tryptophanase cold lability

Sample T (°C) Degree of dissociation (%)
   Tetramer dimmer monomer
P. vulgaris Trpase 25°C 37.3 ± 1.2 44.5 ± 3.4 18.2 ± 4.0
P. vulgaris Trpase 2°C, 24 h 21.7 ± 0.6 45.4 ± 0.7 32.9 ± 1.6
P. vulgaris Trpase +1 mM PLP 25°C, 2 h 92.6 ± 0.8 7.4 ± 0.2 -
E. coli Trpase 25°C 30.0 ± 2.2 70.0 ± 5.0 -
E. coli Trpase + 1 mM PLP 25°C, 1 h 100 ± 0.6 - -
  1. Data in parenthesis correspond to the high resolution shell.