Figure 2

Evolutionary trace analysis. Traces of partitions P01 to P10 aligned with amino acid sequences of the Axin DIX domain structure (1WSPb = chain B of the asymmetric unit; residues 749-830) and the human Dishevelled DIX domain comparative model (DIX; residues 1-83). Conserved residues are boxed, class-specific residues are denoted by X, solvent-exposed side-chains are shaded grey. The secondary structure assignment of the Axin DIX domain is given below the trace. # coloured cyan are sites of non-synonymous amino acid substitution in Axin that have no effect on or only diminished ability to dimerize and * coloured cyan are unable to dimerize [12]. * coloured black are sites of non-synonymous amino acid substitution in Dishevelled that lead to a loss-of-function [29], whereas * coloured red are residues constituting the putative Dishevelled lipid-binding motif and * coloured green are required for Dishevelled actin-binding [28, 30].