The structural and functional determinants of the Axin and Dishevelled DIX domains

BMC Structural Biology

Table 1 Trace residues of the rat Axin1 DIX domain

Axin DIX trace analysis					Residues involved in DIX-DIX interactions^c	Known single non-synonymous substitutions and their consequences
DIX trace residue ^a	CB ^b	SB ^b	CE ^b	SE ^b		Mutant	Characterization	Reference
Y757	X
I764				X	X		Required for homo-dimerization	[14]
P765	X
T768				X
L777	X
Q779				X
F780	X					F780R ^d	Cannot form Axin homodimer	[12]
K781			X
K795			X
V797				X
S798		X
E800				X	X			[14]
F801			X
V805		X
V806			X
F807				X	X	F807R ^d	Cannot form Axin homodimer	[12, 14]
E808				X	X		Required for homo-dimerization	[14]
E809		X
D813			X
L817		X				L817E ^d	Cannot form Axin homodimer	[12, 14]
P818	X
F820		X
E821				X
E822				X
I824		X
I825		X
G826		X
K827				X	X		Required for homo-dimerization	[14]

a numbering according to rat Axin1 DIX crystal structure (1WSP)
b CB, conserved and buried; SB, class-specific and buried; CE, conserved and exposed; SE, class-specific and exposed
c based of contacts observed in the rat Axin 1 crystal structure between chain B and C of the asymmetric unit
d mouse Axin 1 mutants

ISSN: 1472-6807