Figure 2

The interaction interface between the acceptor stem of Met-tRNA _i and domain IV of eIF5B. Interactions between Met-tRNA_i (purple) and eIF5B domain IV (A-D), Phe-tRNA^Phe and domain II of EF-Tu (PDB: 1TTT) (E) and fMet-tRNA^fMet and IF2 domain IV (F). A) Domain IV of eIF5B in ribbon presentation (yellow) with residues potentially involved in interactions with the tRNA as green sticks. B) Surface presentation of domain IV, revealing the two well defined pockets below loop L4 that are also visible on the surfaces of EF-Tu domain II (E, right) and IF2 domain IV (F, right), and might accommodate A76 and the methionyl moiety of the 3′ CCA-end. C) Electrostatic surface potential of domain IV. D) Conservation of residues lying in the proposed interaction interface to the acceptor stem. E) Position of the acceptor stem of Phe-tRNA^Phe (purple) on the surface of domain II of EF-Tu (yellow; PDB: 1TTT). F) Model of domain IV of IF2 (PDB: 1D1N) and the initiator tRNA positioned as in A). The green surfaces indicate residues of IF2 that were shown to interact with fMet-tRNA^fMet[11],[12].