Structural basis of RGD-hirudin binding to thrombin: Tyr3 and five C-terminal residues are crucial for inhibiting thrombin activity

Table 1 Primers used for the generation of RGD-hirudin mutants

Primer	Primer sequence*
Y3A-5′	GAGAAAAGAGTTGTTGCT ACTGACTGCACTGAA
Y3A-3′	TTCAGTGCAGTCAGTAGC AACAACTCTTTTCTC
S50A-5′	ACCCCGAAACCGCAGGCT CACAACCAGGGTGAC
S50A-3′	GTCACCCTGGTTGTGAGC CTGCGGTTTCGGGGT
Q53A-5′	CCGCAGTCCCACAACGCT GGTGACTTCGAACCG
Q53A-3′	CGGTTCGAAGTCACCAGC GTTGTGGGACTGCGG
D55A-5′	TCCCACAACCAGGGTGCT TTCGAACCGATCCCG
D55A-3′	CGGGATCGGTTCGAAAGC ACCCTGGTTGTGGGA
E57A-5′	AACCAGGGTGACTTCGCT CCGATCCCGGAAGAC
E57A-3′	GTCTTCCGGGATCGGAGC GAAGTCACCCTGGTT
I59A-5′	GGTGACTTCGAACCGGCT CCGGAAGACGCTTAC
I59A-3′	GTAAGCGTCTTCCGGAGC CGGTTCGAAGTCACC

ISSN: 1472-6807