Skip to main content

Advertisement

Table 1 Primers used for the generation of RGD-hirudin mutants

From: Structural basis of RGD-hirudin binding to thrombin: Tyr3 and five C-terminal residues are crucial for inhibiting thrombin activity

Primer Primer sequence*
Y3A-5′ GAGAAAAGAGTTGTTGCT ACTGACTGCACTGAA
Y3A-3′ TTCAGTGCAGTCAGTAGC AACAACTCTTTTCTC
S50A-5′ ACCCCGAAACCGCAGGCT CACAACCAGGGTGAC
S50A-3′ GTCACCCTGGTTGTGAGC CTGCGGTTTCGGGGT
Q53A-5′ CCGCAGTCCCACAACGCT GGTGACTTCGAACCG
Q53A-3′ CGGTTCGAAGTCACCAGC GTTGTGGGACTGCGG
D55A-5′ TCCCACAACCAGGGTGCT TTCGAACCGATCCCG
D55A-3′ CGGGATCGGTTCGAAAGC ACCCTGGTTGTGGGA
E57A-5′ AACCAGGGTGACTTCGCT CCGATCCCGGAAGAC
E57A-3′ GTCTTCCGGGATCGGAGC GAAGTCACCCTGGTT
I59A-5′ GGTGACTTCGAACCGGCT CCGGAAGACGCTTAC
I59A-3′ GTAAGCGTCTTCCGGAGC CGGTTCGAAGTCACC
  1. * Sense primer-5′; anti-sense primer-3′; all mutations are in bold letters.