Fig. 7
From: New insights into the molecular mechanism of the Rab GTPase Sec4p activation
Sec2 residues 1–142 are sufficient and necessary for cell viability; a construct containing this region of Sec2 can provide the sole source of SEC2 function in a tester strain on 5-FOA media. Truncation of the first fifty residues abolishes this effect, which is evident in the absence of colonies on the 5-FOA media in the Sec2 51–142 construct. Vector only and SEC2 wild type constructs are shown for reference