Fig. 4
From: An intact helical domain is required for Gα14 to stimulate phospholipase Cβ
An intact N-terminal and helical domain are required for Gα14 mediated PLCβ interaction and activation. a Schematic representation of the 14z224, 131z14, 14αDEF and zαDEF chimeras. b, Cells were co-transfected with PLCβ2 and the indicated chimeras. Co-immunoprecipitation assays were performed and analyzed as in Fig. 2. Data shown represent one of three sets of immunoblots; two other sets yielded similar results. c HEK293 cells were transiently transfected with the wild-type or constitutively active mutants (QL) of Gα protein or the indicated chimeras and then subjected to IP3 accumulation assay and analyzed as in Fig. 2. *, IP3 production was significantly enhanced as compared to corresponding wild-type transfected cells; Dunnett t test, p < 0.05