Fig. 3
ATP binding motif and ATPase activity of UspE. a Close-up view of the b Alignment of the sequences around the ATP binding motif from E. coli UspE (Ec UspE), P. mirabilis (Pm UspE), P. aeruginosa UspE (Pa UspE), and M. tuberculosis (Mt UspE). The conserved residues and ATP binding motif are highlighted. c Kinase activity of UspE. Varying concentrations (0.4, 0.8, 1.6, 3.2, 6.4, mg/ml) of UspE were used in the reaction mixture, and the protein are resuspended in 50 μl containing 10 mM MgCl2 and 5 μM ATP. The graph represents the mean of three independent experiments, and the standard deviation is indicated by error bars