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Table 1 SAXS data-collection and scattering-derived parameters

From: Combined small angle X-ray solution scattering with atomic force microscopy for characterizing radiation damage on biological macromolecules

Data-collection parameters
Instrument ESRF BM29
Beam Geometry 0.7 mm ×0.7 mm at sample
Wavelength (nm) 0.099
q range (nm −1) 0.05-4.95
Exposure time 1 s per frame
Concentration, β-amylase 5.5 mg/mL
Concentration, Tobacco Mosaic Virus 26 mg/mL
Temperature (K) 293
Structural Parameters, β-amylase
Exposure time 1 s 10 s (standard) 5 min 30 min
I 0 (cm −1) [from Guinier] 0.135 ± 0.001 0.135 ± 0.001 0.133 ± 0.1 0.142 ± 0.001
R g (nm) [from Guinier] 4.14 ± 0.06 4.14 ± 0.06 4.16 ± 0.06 4.35 ± 0.13
I 0 (cm −1) [from P(r)] 0.134 0.135 0.135 0.132
R g (nm) [from P(r)] 4.09 4.10 4.12 4.22
D max (nm) 1.10 1.13 1.20 1.30
Porod volume estimate (nm 3) 267 267 266 283
Structural Parameters, Tobacco Mosaic Virus
Exposure time 1 s 10 s (standard) 5 min 30 min
I 0 (cm −1 x nm −1) 0.312 ± 0.003 0.325 ± 0.003 0.330 ± 0.003 0.317 ± 0.003
[from cross-sectional Guinier]     
cross-sectional R g (nm) 6.34 6.40 6.51 6.49
[from cross-sectional Guinier]     
I 0 (cm −1 x nm −1) 0.278 0.284 0.289 0.279
[from cross-sectional P(r)]     
cross-sectional R g (nm) [from cross-sectional P(r)] 5.89 5.89 5.88 5.87
cross-sectional D max (nm) 18.0 18.0 18.0 18.0
Molecular-mass determination, β-amylase
Exposure time 1 s 10 s (standard) 5 min 30 min
Partial specific volume (cm 3 g −1) 0.724
Contrast (Δ ρ×1010 cm −2) 3.047
Molecular mass M r (kDa) [from I 0] 168 ± 1 170 ± 1 167 ± 1 178 ± 1
Molecular mass M r (kDa) 157 157 156 166
[from Porod Volume] [64]     
Calculated molecular weight according to sequence (kDa) 224
Software employed
Primary data reduction BM29 online data analysis [65],
  pyFAI [66], Primus [64]
1D data processing PRIMUS
p(r) analysis GNOM [42]
Form factor fitting GENFIT [43]
Computation of model intensities CRYSOL [44]
Computation of volume fractions of mixtures OLIGOMER [64]