Fig. 6

The disordered nature of Pup covalently ligated to a substrate allows for interaction with different Pup binding partners. Schematic representation of the disordered behavior of Pup (red) in its free form and when attached to a substrate (grey). The intrinsically disordered nature of Pup in its free as well as substrate-ligated form allows interaction with multiple binding partners such as the ligase PafA (blue), the deamidase/depupylase Dop (green), the mycobacterial proteasomal ATPase Mpa (orange) or additional unknown potential interaction partners