Table 1 Primers used in this study
Constructs/Variants | Primer | Nr | Sequence of PCR primer (5'-3', restriction sites: italic, mutations/insertions: underlined) |
|---|---|---|---|
His6-TEV-PanB | fwd | P1 | ATTAGTTATCTTAAGATGTCGGAGCAGACTATCTAT |
His6-TEV-PanB | rev | P2 | AATTTATATCCTAGGTTAGAAACTGTGTTCGTCAGC |
PanB-K212A | fwd | P3 | CCCAGATCACCGGCGCGCTTACCATTCCGACGG |
PanB-K212A | rev | P4 | TGGCCAACTCGGCGGGCACCATCTCCATCACGAC |
His6-TEV-FabD | fwd | P5 | GAGATATACATATGCACCATCACCATCACCATGGCGAGAATCTTTATTTTCAGGGCATGATTGCGTTGCTGGCACCC |
His6-TEV-FabD | rev | P6 | ATATTATCCTAGGTTATAGGTTTGCCAGCTCGTCCAGGT |
His6-TEV-GG-FabD | fwd | P7 | ATGATTGCGTTGCTGGCACCCGGA |
His6-TEV-GG-FabD | rev | P8 | ACCGCCGCCCTGAAAATAAAGATTCTCGCC |
FabD-K35R | fwd | P9 | GATCTTGCCCGGCTGGGCACCACC |
FabD-K35R | rev | P10 | TAGATCAGCGGCACGCGACCACGC |
FabD-K122R | fwd | P11 | CGCGGCGCCGAGATGGCCCGCGCCTGCGCCACCGAGCCG |
FabD-K122R | rev | P12 | CGGCTCGGTGGCGCAGGCGCGGGCCATCTCGGCGCCGCG |
FabD-K181R | fwd | P13 | GACCCGCCGGCCCGCGCGCGGGTGCGT |
FabD-K181R | rev | P14 | TTCGGCGAGCTTCTCCAACGCGGTCAG |