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Table 1 Summary of the hydrodynamic data obtained from DLS and AUC experiments.

From: Central domain deletions affect the SAXS solution structure and function of Yeast Hsp40 proteins Sis1 and Ydj1

Hydrodynamic properties

Protein

 

Sis1

Sis1_Δ 124-174

Sis1_Δ 121-257

Ydj1_Δ 106-255

Predicted M (kDa)*

75.2

66.1

46.8

56.1

Experimental M (kDa)

64 ± 3€

58 ± 2# 58 ± 1€

45 ± 2# 43 ± 1€

54 ± 1# 55 ± 2€

s020,w (S)#

3.5 ± 0.1%

3.4 ± 0.1

3.0 ± 0.1

3.6 ± 0.1

s sph (S)

6.5

5.9

4.7

5.2

D20,w (10-7cm2/seg)

5.1 ± 0.2

5.4 ± 0.1

6.3 ± 0.1

5.9 ± 0.2

Dsph (10-7cm2/seg)

7.7

8.0

9.0

8.45

Æ’/Æ’ 0 #

1.6 ± 0.1

1.6 ± 0.1

1.5 ± 0.1

1.4 ± 0.1

  1. * M predicted from amino acids sequence for all proteins as dimers.
  2. # data from SV experiments fitted by SedFit software (see Material and methods for details);
  3. € M calculated from s/D ratio as showed by Equation 4;
  4. % data from [19].
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BMC Structural Biology

ISSN: 1472-6807

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