Skip to main content

Table 1 Summary of the hydrodynamic data obtained from DLS and AUC experiments.

From: Central domain deletions affect the SAXS solution structure and function of Yeast Hsp40 proteins Sis1 and Ydj1

Hydrodynamic properties Protein
  Sis1 Sis1_Δ 124-174 Sis1_Δ 121-257 Ydj1_Δ 106-255
Predicted M (kDa)* 75.2 66.1 46.8 56.1
Experimental M (kDa) 64 ± 3 58 ± 2# 58 ± 1 45 ± 2# 43 ± 1 54 ± 1# 55 ± 2
s020,w (S)# 3.5 ± 0.1% 3.4 ± 0.1 3.0 ± 0.1 3.6 ± 0.1
s sph (S) 6.5 5.9 4.7 5.2
D20,w (10-7cm2/seg) 5.1 ± 0.2 5.4 ± 0.1 6.3 ± 0.1 5.9 ± 0.2
Dsph (10-7cm2/seg) 7.7 8.0 9.0 8.45
ƒ/ƒ 0 # 1.6 ± 0.1 1.6 ± 0.1 1.5 ± 0.1 1.4 ± 0.1
  1. * M predicted from amino acids sequence for all proteins as dimers.
  2. # data from SV experiments fitted by SedFit software (see Material and methods for details);
  3. M calculated from s/D ratio as showed by Equation 4;
  4. % data from [19].