Structural analysis of hemicatenated DNA loops
© Gaillard et al; licensee BioMed Central Ltd 2002
Received: 7 September 2002
Accepted: 26 November 2002
Published: 26 November 2002
We have previously isolated a stable alternative DNA structure, which was formed in vitro by reassociation of the strands of DNA fragments containing a 62 bp tract of the CA-microsatellite poly(CA)·poly(TG). In the model which was proposed for this structure the double helix is folded into a loop, the base of the loop consists of a DNA junction in which one of the strands of one duplex passes between the two strands of the other duplex, forming a DNA hemicatenane in a hemiknot structure. The hemiknot DNA structures obtained with long CA/TG inserts have been imaged by AFM allowing us to directly visualize the loops.
Here we have analyzed this structure with several different techniques: high-resolution gel electrophoresis, probing by digestion with single stranded DNA-specific nucleases or with DNase I, modification with chemicals specific for unpaired bases, and atomic force microscopy. The data show a change in DNA structure localized to the CA/TG sequence and allow us to better understand the structure of this alternative conformation and the mechanism of its formation.
The present work is in good agreement with the model of hemicatenated DNA loop proposed previously. In the presence of protein HMGB1, shifted reassociation of the strands of DNA fragments containing a tract of the poly(CA)·poly(TG) microsatellite leads to the formation of DNA loops maintained at their base by a hemicatenated junction located within the repetitive sequence. No mobility of the junction along the DNA molecule could be detected under the conditions used. The novel possibility to prepare DNA hemicatenanes should be useful to further study this alternative DNA structure and its involvement in replication or recombination.
DNA can adopt alternative conformations which can be very different from the classical B-form double helix. As infrequent as they may be inside the cell, these conformations are of interest since DNA replication and recombination are two processes during which intermediates are formed that present a wide structural diversity. In addition, given the structural uniformity of the classical B-form double helix, alternative DNA conformations might serve as signals presenting a strong structural contrast with the rest of the DNA molecule and allowing a straightforward localization of certain specific sites on the genome.
The possibility for DNA to form hemicatenanes has been discussed and documented in relation with recombination [12–22] or replication [23–26]. However, hemicatenanes have been difficult to study due to the lack of a technique to prepare them. Now that a method permits the preparation of significant amounts of hcDNA , we have studied this structure by high-resolution gel electrophoresis, chemical and enzymatic probing, and direct imaging using AFM. The results allow us to discuss and to refine the model, and to support the mechanism proposed for the formation of hemicatenated DNA loops and hemiknot DNA structures.
High resolution electrophoresis
In an attempt to detect structural differences between the different bands, this analysis was refined using two-dimensional electrophoresis. After the first dimension electrophoresis on 8% gel, gel slices were cut and loaded on a second dimension 4% gel either directly, or after DNA denaturation by incubating the gel slice for 5 min at 100°C. The analysis of the non-denatured material (Fig. 2B, left panel) shows at least 10 spots, some of which are very weak and could not be detected in the first dimension. After heat-denaturation, all the spots are found to contain both strands of the DNA fragment in stoichiometric amounts (Fig. 2B, right panel). Therefore, no change of conformation during electrophoresis can be detected and the 2-dimension gels show that hcDNA consists in a series of stable alternative conformations of the DNA fragment.
To get more information about the structure of hcDNA and the differences between the different bands, hcDNA was then studied with enzymatic and chemical probes capable of sensing alternative DNA conformation.
S1 and P1 probing
The model (Fig. 1) involving a partial opening of the double helix at the hemicatenated junction, we probed hcDNA with S1 nuclease, the most classical enzyme for single-stranded DNA regions. For the experiment shown in Figure 3, the DNA fragment was labeled at one end and converted into hcDNA that was fractionated on polyacrylamide gels. Total hcDNA was purified on a 4% gel, while the two major hcDNA bands were purified in parallel on an 8% gel (see Fig. 2A, first and second band from the top). hcDNA was digested with S1 nuclease and DNA analyzed on a denaturing polyacrylamide-urea gel. Linear DNA was digested in parallel as a control, and a G+A chemical reaction on the same fragment was used as a size marked. The results (Fig. 3A) clearly show that hcDNA is preferentially digested in the poly(CA)·poly(TG) region, no preferential digestion can be seen outside of the repeat. The patterns of digestion of bands 1 and 2 and of total hcDNA are very similar and do not point to any major structural difference between the two major bands. A striking feature of the digestion pattern is the fact that 10–12 bases on each side in the repetitive region are not cut by the enzyme, suggesting that the opening of the double helix in hcDNA never extends to the very side of the poly(CA)·poly(TG) sequence.
In summary, both S1 and P1 nucleases cut hcDNA in the repetitive region only, with flanks of about 10 bp of the CA sequence being resistant to digestion. With P1 nuclease the middle of the repeat is somewhat less accessible to the enzyme. The presence of HMGB1 reinforces this pattern.
Enzymes used as structural probes are high molecular weight molecules that sense the nature of the substrate as well as its steric accessibility. Small molecules are less influenced by steric effects, and are thus very useful for structural studies. We have used hydroxylamine, which reacts with cytosines residues in a manner that allows alkaline cleavage of the backbone. Hydroxylamine is very reactive with single-stranded DNA and practically unreactive with double-stranded DNA having a uniform structure, except at structural discontinuities (for example at B-Z junctions [27, 28]).
Even though the lack of sensitivity on the sides of the repetitive sequence was not expected, it is immediately obvious that these results are in good agreement with the model of a hemicatenated loop (Fig. 1), which predicts the presence of two open regions flanking the center of the repetitive sequence. To try to get more structural details allowing us to refine the model we also used other reagents to probe hcDNA.
Probing with DNase I
Atomic force microscopy
hcDNA, an alternative DNA structure obtained by reassociation of the strands of a DNA fragment containing a tract of poly(CA)·poly(TG), is composed of a population of stable conformations. On high resolution polyacrylamide gels, hcDNA is resolved in a series of defined bands each of which contains both strands of the DNA fragment in equal amounts, the possibility that some of the bands contain only one of the single strands or a triple-stranded structure can thus be ruled out. The similarity of all the bands may only be apparent and should not be taken to imply that all the bands share the same basic structure, especially in view of the fact that no interconversion between the different bands could be observed under the conditions used. On the other hand however, given that about 10 bands could be resolved which all contain knotted structures, it is unlikely that they all consist in different structures, instead it seems likely that many of them share the same basic conformation, while differing by some parameters of the structure. In agreement with this suggestion is the fact that the experiments performed on isolated bands gave results similar to those obtained with the bulk of hcDNA. The situation would then be somewhat similar to topoisomers of a circular DNA molecule of a given size, where moderate changes of a single parameter of the structure, in this case the DNA supercoiling density, can lead to a series of bands with markedly different electrophoretic mobilities on concentrated polyacrylamide gels.
For example in the model which was proposed for hcDNA of DNA loops maintained by a hemicatenane at the DNA junction at the base of the loops, several parameters of the structure can vary, including the precise size of the loop, the winding of DNA inside the loop, and the loop position along the DNA sequence. With an overall structure remaining globally the same, such minor variations should be expected to lead to quantized changes in electrophoretic mobility. In particular, the loop size should have an important effect on the mobility in high percentage gels, even more than with DNA minicircles since different loop sizes might result in variations of the angle between the two arms. Consistent with the model, it is interesting to note that the absence of interconversion between the different forms which is observed here is no longer true at higher temperatures, since some changes in the pattern of bands of hcDNA with ends closed by ligation of hairpin oligonucleotides are observed upon incubation at 100°C (see Fig. 4 in ).
The data obtained with nucleases S1 and P1 and with hydroxylamine are also in excellent agreement with the model (Fig. 1). In the hemicatenated DNA loop, two regions of the DNA duplex are open since in the junction one strand of each duplex passes between the strands of the other duplex. The model thus predicts the existence of two sensitive regions, on each side of the loop, flanking a region of lower sensitivity which comprises the loop. This is exactly what is observed in the experiments with P1 nuclease and with hydroxylamine. S1 nuclease gives similar results, except that no reduction of sensitivity is observed in the center of the repeat, which may be due to the different pH conditions during digestion.
At first, the results could also be interpreted as showing the presence of staggered denaturation loops on each side of the CA/TG tract, with one single-stranded loop on one side on the CA strand and another loop on the other side on the TG strand. Such a model can be excluded, however, on the basis of our previous data on the topology of DNA strands in hcDNA . In particular, measurements of the DNA linking number in circularized hcDNA , and data showing that hcDNA molecules with their ends covalently closed by ligation of hairpin oligonucleotides are absolutely stable and are resistant to denaturation both by heat and by sodium hydroxyde , are absolutely incompatible with this hypothesis. Actually, in all our experiments with DNA fragments containing a CA microsatellite, we have never observed a structure that might have corresponded to staggered denaturation loops. It is very likely that if such structures exist they are extremely unstable; sliding of the loops requiring no energy they can rejoin very easily to reform the fully base-paired double-stranded fragment.
Other models can be considered as long as they take into account the presence of a knot in the structure. For example, given the extremely short persistence length of single-stranded DNA, the possibility exists that a knot forms on one of the strands and becomes trapped by the reassociation of the opposite strand. The presence of a knot, by locally preventing the complete reassociation of the strands, would lead to the formation of regions sensitive to single strand-specific reagents, as observed. In such a case, however, the important flexibility of single-stranded DNA should be expected to favor the formation of loops significantly smaller than the length reduction observed by AFM. And in this model it is more difficult to explain than in a shifted-pairing model why the unpaired regions are strictly restricted to the repetitive sequence.
The loop size is particularly small in hcDNA. With a length of 62 bp for the CA/TG tract, the data obtained with single strand-specific reagents suggest a loop size of less than 40 bp which is in good agreement with the average of 39 bp from length measurements of AFM images . This strong curvature of DNA cannot be opposed to the model, however, since DNA is known to accommodate highly curved structures. In the nucleosome, DNA makes 1.7 turn for 146 bp, i.e. 85 bp per turn . And in crystals of some complexes of DNA with proteins much important deformations of the double helix are frequently observed, resulting in strong kinks or writhes of DNA localized on regions which can be just a few base pairs long . Therefore a very strong curvature is well tolerated by DNA as long as it is stabilized by proteins or by circularization. As discussed previously , the formation of loops much smaller than the value of 150 bp for the persistence length of DNA  is probably due to the flexibility of poly(CA)·poly(TG) [32–34] and to the fact that HMGB1 facilitates strongly the circularization of short DNA fragments [35, 36] since DNA fragments as short as 59 bp could be circularized by T4 DNA ligase in the presence of HMGB1 . Thus the small size of the loop is not in contradiction with the model, but it provides a possible explanation for the variations of the pattern of digestion with DNase I, since the constraints induced by such a curvature cannot be without any influence on the sensitivity of DNA to the enzyme.
The presence of two regions on the sides in the repetitive sequence which are not sensitive to the single strand-specific enzymes and reagents was not predicted by the initial model (Fig. 1). This suggests that the junction is never localized on the sides of the repetitive sequence but is at a distance of at least 10 nucleotides from each border between the repetitive and non-repetitive sequences, and has implications as regards the mobility of hemicatenated junctions along the DNA molecule, as well as the mechanism of formation of the loops.
First, this observation suggests that under the conditions of the experiments the hemicatenated junction cannot move by translation along the DNA sequence in hcDNA, and in particular that it never moves outside of the CA/TG sequence, confirming our previous AFM observations . This assumption is also supported by the fact that no interconversion between bands is observed (Fig. 2), implying that even inside the repetitive region no displacement of the junction can occur. A likely possibility is that such displacements are made difficult by the fact that the inside strands in the junction are strongly held between the opposite strands, like in clamps.
During the initial steps (Fig. 7,7A,7B), the length of the repetitive sequence, 62 bp in the experiments shown here, is probably sufficiently short to prevent the formation of hairpins as observed upon reassociation of long strands of poly(CA) and poly(TG)  and of the complex hemiknot structures observed with a 188 bp CA/TG repeat . The precise mechanism of the next step, which follows the initial shifted pairing of the CA and TG strands, will have to be investigated. In particular, the presence of the central double-stranded region should interfere with the winding of the single strands on the sides (Fig. 7,7B) and prevent the formation of a regular B-form double helix in this region of the structure, as already suggested by the diversity of AFM images of hemiknots obtained with a fragment containing a 185 bp CA/TG insert . During this step, the DNA conformation at the base of the loop might be reminiscent of form V DNA  and involve some left-handed parts  since poly(CA)·poly(TG) is known to convert easily to Z-DNA [40, 41]. Studies performed currently with other repetitive sequences that do not convert to Z-DNA easily should bring information on this point.
Finally, an additional possibility to explain the stability of the hemicatenated DNA loops and the fact that translations of the loops are not observed is that base pairing might occur between the opposite single strands inside the junction and contribute to its stability. Indeed, two regions that were initially distant on the linear molecule are brought into close proximity inside the junction, and the possibility that some base pairing occurs between these sequences is particularly interesting since it would allow direct, stable, sequence-specific interactions between two distant regions of the double-stranded DNA molecule.
High-resolution electrophoresis, enzymatic and chemical probing, and atomic force microscopy, are in agreement with the model of hemicatenated DNA loops which was initially proposed  and support the mechanism suggested for the formation of these structures [7, 8]. Shifted reassociation of the strands of DNA fragments containing a tract of the poly(CA)·poly(TG) microsatellite leads to the formation of DNA loops maintained at their base by a hemicatenated junction located in the repetitive tract. A novel possibility is thus offered to prepare DNA hemicatenanes, providing a new way to study the possible function of hemicatenanes in processes such as recombination and replication which involve the formation of alternative DNA structures. Finally, the data suggest that the partial opening of DNA helices inside a hemicatenane might allow for sequence-specific interactions between two distant regions of the DNA molecule.
DNA fragments were extracted from plasmid pE10 (accession number X96980) amplified in E. coli. This plasmid contains a tract of 62 bp of poly(CA)·poly(TG) inserted in the polylinker of pUC19. After digestion by the restriction enzymes Eco RI and Cla I, DNA fragments were purified by polyacrylamide gel electrophoresis and electroelution. The map of the fragment is shown on Figure 1.
For most experiments, DNA fragments were dephosphorylated with alkaline phosphatase and labeled at their 5' end using polynucleotide kinase in the presence of [γ-32P]-ATP. For the experiments with S1 nuclease, as this enzyme shows a strong preference for the end of fragments which would lead to a quick loss of the 5'-32P label, DNA fragments were labeled with the Klenow fragment of DNA polymerase I in the presence of [α-32P]-dATP.
Preparation of hemicatenanes
DNA hemicatenanes were prepared and purified as described previously . In brief, the strands of the labeled DNA fragment are separated by thermal denaturation and reannealed at 37° in the presence of protein HMGB1, yielding the complex of hemicatenanes with HMGB1. After chloroform extraction to remove the protein and ethanol precipitation, hemicatenanes are purified by preparative polyacrylamide gel electrophoresis and electroelution, with a global yield of 5–10%.
Two kinds of gels were used for the fractionation and purification of hemicatenated loops. Low resolution gels were 4% polyacrylamide gels (acrylamide:bis-acrylamide 29:1) in 6.7 mM Tris-acetate, 3.3 mM Na-acetate, 1 mM EDTA, pH 7.8, at 4°C with buffer recirculation. High-resolution gels were 8% polyacrylamide gels in 40 mM Tris-acetate, 20 mM Na-acetate, 1 mM EDTA, pH 7.8. On 8% gels the electrophoretic mobility of hemicatenated loops is much lower but they give a much better separation of the different conformations (see Fig. 2). For two-dimensional electrophoresis, the slice of the first dimension gel was cut and loaded on top of the second gel, either directly, or after a 5-min incubation in electrophoresis buffer at 100°C to denature DNA.
Chemical treatment of DNA
Treatment of DNA with hydroxylamine and subsequent cleavage by piperidine at the level of modified bases were performed as described .
Atomic Force Microscopy
Aminopropylsilatrane-mica (APS-mica) was obtained by the treatment of freshly cleaved mica (Asheville-Schoomaker Mica Co., Newport News, VA) in 50 mM solution of aminopropyl silatrane as described earlier . The AFM imaging procedure has been described elsewhere [43, 8]. Briefly, DNA samples (3–5 μl) were placed onto APS-mica for 2 min, and the mica was rinsed with deionized water, after which the specimens were dried in an argon flow. Images were acquired by MM SPM NanoScope IIIa system (Veeco/Digital Instruments, Santa Barbara, CA) operating in Tapping Mode in air at ambient conditions using OTESPA probes (Digital Instruments, Inc.).
This research was supported by the Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie (Paris 6), and Université Denis Diderot (Paris 7).
- Gaillard C, Strauss F: Association of poly(CA).poly(TG) DNA fragments into four-stranded complexes bound by HMG1 and 2. Science 1994, 264: 433–436.View ArticlePubMedGoogle Scholar
- Belotserkovskii BP, Johnston BH: Polypropylene tube surfaces may induce denaturation and multimerization of DNA. Science 1996, 271: 222–223.View ArticlePubMedGoogle Scholar
- Belotserkovskii BP, Johnston BH: Denaturation and association of DNA sequences by certain polypropylene surfaces. Anal Biochem 1997, 251: 251–262. 10.1006/abio.1997.2249View ArticlePubMedGoogle Scholar
- Gaillard C, Flavin M, Woisard A, Strauss F: Association of double-stranded DNA fragments into multistranded DNA structures. Biopolymers 1999, 50: 679–689. 10.1002/(SICI)1097-0282(199912)50:7<679::AID-BIP1>3.0.CO;2-3View ArticlePubMedGoogle Scholar
- Yakubovskaya MG, Neschastnova AA, Humphrey KE, Babon JJ, Popenko VI, Smith MJ, Lambrinakos A, Lipatova ZV, Dobrovolskaia MA, Cappai R, Masters CL, Belitsky GA, Cotton RG: Interaction of linear homologous DNA duplexes via Holliday junction formation. Eur J Biochem 2001, 268: 7–14. 10.1046/j.1432-1327.2001.01861.xView ArticlePubMedGoogle Scholar
- Neschastnova AA, Markina VK, Popenko VI, Danilova OA, Sidorov RA, Belitsky GA, Yakubovskaya MG: Mechanism of spontaneous DNA-DNA interaction of homologous linear duplexes. Biochemistry 2002, 41: 7795–7801. 10.1021/bi015959tView ArticlePubMedGoogle Scholar
- Gaillard C, Strauss F: DNA loops and semicatenated DNA junctions. BMC Biochem 2000, 1: 1. 10.1186/1472-2091-1-1PubMed CentralView ArticlePubMedGoogle Scholar
- Lyubchenko YL, Shlyakhtenko LS, Binus M, Gaillard C, Strauss F: Visualization of hemiknot DNA structure with an atomic force microscope. Nucleic Acids Res 2002, 30: 4902–4909. 10.1093/nar/gkf626PubMed CentralView ArticlePubMedGoogle Scholar
- Bustin M: Revised nomenclature for high mobility group (HMG) chromosomal proteins. Trends Biochem Sci 2001, 26: 152–153. 10.1016/S0968-0004(00)01777-1View ArticlePubMedGoogle Scholar
- Bianchi ME, Beltrame M: Flexing DNA: HMG-box proteins and their partners. Am J Hum Genet 1998, 63: 1573–1577. 10.1086/302170PubMed CentralView ArticlePubMedGoogle Scholar
- Gaillard C, Strauss F: High affinity binding of proteins HMG1 and HMG2 to semicatenated DNA loops. BMC Mol Biol 2000, 1: 1. 10.1186/1471-2199-1-1PubMed CentralView ArticlePubMedGoogle Scholar
- Cunningham RP, Wu AM, Shibata T, DasGupta C, Radding CM: Homologous pairing and topological linkage of DNA molecules by combined action of E. coli RecA protein and topoisomerase I. Cell 1981, 24: 213–223. 10.1016/0092-8674(81)90517-1View ArticlePubMedGoogle Scholar
- Bianchi M, DasGupta C, Radding CM: Synapsis and the formation of paranemic joints by E. coli RecA protein. Cell 1983, 34: 931–939. 10.1016/0092-8674(83)90550-0View ArticlePubMedGoogle Scholar
- Kmiec EB, Holloman WK: Homologous pairing of DNA molecules by Ustilago rec1 protein is promoted by sequences of Z-DNA. Cell 1986, 44: 545–554. 10.1016/0092-8674(86)90264-3View ArticlePubMedGoogle Scholar
- Christiansen K, Bonven BJ, Westergaard O: Mapping of sequence-specific chromatin proteins by a novel method: topoisomerase I on Tetrahymena ribosomal chromatin. J Mol Biol 1987, 193: 517–525.View ArticlePubMedGoogle Scholar
- Umlauf SW, Cox MM, Inman RB: Triple-helical DNA pairing intermediates formed by recA protein. J Biol Chem 1990, 265: 16898–16912.PubMedGoogle Scholar
- McKee BD, Habera L, Vrana JA: Evidence that intergenic spacer repeats of Drosophila melanogaster rRNA genes function as X-Y pairing sites in male meiosis, and a general model for achiasmatic pairing. Genetics 1992, 132: 529–544.PubMed CentralPubMedGoogle Scholar
- Schwacha A, Kleckner N: Identification of joint molecules that form frequently between homologs but rarely between sister chromatids during yeast meiosis. Cell 1994, 76: 51–63. 10.1016/0092-8674(94)90172-4View ArticlePubMedGoogle Scholar
- Schwacha A, Kleckner N: Identification of double Holliday junctions as intermediates in meiotic recombination. Cell 1995, 83: 783–791. 10.1016/0092-8674(95)90191-4View ArticlePubMedGoogle Scholar
- Wong BC, Chiu SK, Chow SA: The role of negative superhelicity and length of homology in the formation of paranemic joints promoted by RecA protein. J Biol Chem 1998, 273: 12120–12127. 10.1074/jbc.273.20.12120View ArticlePubMedGoogle Scholar
- Polanco C, Gonzalez AI, Dover GA: Patterns of variation in the intergenic spacers of ribosomal DNA in Drosophila melanogaster support a model for genetic exchanges during X-Y pairing. Genetics 2000, 155: 1221–1229.PubMed CentralPubMedGoogle Scholar
- Kajander OA, Karhunen PJ, Holt IJ, Jacobs HT: Prominent mitochondrial DNA recombination intermediates in human heart muscle. EMBO Rep 2001, 2: 1007–1012. 10.1093/embo-reports/kve233PubMed CentralView ArticlePubMedGoogle Scholar
- Sogo JM, Stahl H, Koller T, Knippers R: Structure of replicating simian virus 40 minichromosomes. The replication fork, core histone segregation and terminal structures. J Mol Biol 1986, 189: 189–204.View ArticlePubMedGoogle Scholar
- Laurie B, Katritch V, Sogo J, Koller T, Dubochet J, Stasiak A: Geometry and physics of catenanes applied to the study of DNA replication. Biophys J 1998, 74: 2815–2822.PubMed CentralView ArticlePubMedGoogle Scholar
- Lucas I, Hyrien O: Hemicatenanes form upon inhibition of DNA replication. Nucleic Acids Res 2000, 28: 2187–2193. 10.1093/nar/28.10.2187PubMed CentralView ArticlePubMedGoogle Scholar
- Benard M, Maric C, Pierron G: DNA replication-dependent formation of joint DNA molecules in Physarum polycephalum. Mol Cell 2001, 7: 971–980. 10.1016/S1097-2765(01)00237-4View ArticlePubMedGoogle Scholar
- Johnston BH, Rich A: Chemical probes of DNA conformation: detection of Z-DNA at nucleotide resolution. Cell 1985, 42: 713–724. 10.1016/0092-8674(85)90268-5View ArticlePubMedGoogle Scholar
- Johnston BH: Hydroxylamine and methoxylamine as probes of DNA structure. Methods Enzymol 1992, 212: 180–194. 10.1016/0076-6879(92)12012-FView ArticlePubMedGoogle Scholar
- Luger K, Mader AW, Richmond RK, Sargent DF, Richmond TJ: Crystal structure of the nucleosome core particle at 2.8 A resolution. Nature 1997, 389: 251–260. 10.1038/38444View ArticlePubMedGoogle Scholar
- Dickerson RE: DNA bending: the prevalence of kinkiness and the virtues of normality. Nucleic Acids Res 1998, 26: 1906–1926. 10.1093/nar/26.8.1906PubMed CentralView ArticlePubMedGoogle Scholar
- Hagerman PJ: Flexibility of DNA. Annu Rev Biophys Biophys Chem 1988, 17: 265–286. 10.1146/annurev.biophys.17.1.265View ArticlePubMedGoogle Scholar
- Lyubchenko Y, Shlyakhtenko L, Chernov B, Harrington RE: DNA bending induced by Cro protein binding as demonstrated by gel electrophoresis. Proc Natl Acad Sci U S A 1991, 88: 5331–5334.PubMed CentralView ArticlePubMedGoogle Scholar
- Lyubchenko YL, Shlyakhtenko LS, Appella E, Harrington RE: CA runs increase DNA flexibility in the complex of lambda Cro protein with the OR3 site. Biochemistry 1993, 32: 4121–4127.View ArticlePubMedGoogle Scholar
- Russu IM: Studying DNA-protein interactions using NMR. Trends Biotechnol 1991, 9: 96–104. 10.1016/0167-7799(91)90032-DView ArticlePubMedGoogle Scholar
- Paull TT, Haykinson MJ, Johnson RC: The nonspecific DNA-binding and -bending proteins HMG1 and HMG2 promote the assembly of complex nucleoprotein structures. Genes Dev 1993, 7: 1521–1534.View ArticlePubMedGoogle Scholar
- Pil PM, Chow CS, Lippard SJ: High-mobility-group 1 protein mediates DNA bending as determined by ring closures. Proc Natl Acad Sci U S A 1993, 90: 9465–9469.PubMed CentralView ArticlePubMedGoogle Scholar
- Gibb CL, Cheng W, Morozov VN, Kallenbach NR: Effect of nuclear protein HMG1 on in vitro slippage synthesis of the tandem repeat dTG·dCA. Biochemistry 1997, 36: 5418–5424. 10.1021/bi962037vView ArticlePubMedGoogle Scholar
- Stettler UH, Weber H, Koller T, Weissmann C: Preparation and characterization of form V DNA, the duplex DNA resulting from association of complementary, circular single-stranded DNA. J Mol Biol 1979, 131: 21–40.View ArticlePubMedGoogle Scholar
- Pohl FM, Thomae R, DiCapua E: Antibodies to Z-DNA interact with form V DNA. Nature 1982, 300: 545–546.View ArticlePubMedGoogle Scholar
- Haniford DB, Pulleyblank DE: Facile transition of poly[d(TG)·d(CA)] into a left-handed helix in physiological conditions. Nature 1983, 302: 632–634.View ArticlePubMedGoogle Scholar
- Nordheim A, Rich A: The sequence (dC-dA)nx(dG-dT)n forms left-handed Z-DNA in negatively supercoiled plasmids. Proc Natl Acad Sci USA 1983, 80: 1821–1825.PubMed CentralView ArticlePubMedGoogle Scholar
- Shlyakhtenko LS, Potaman VN, Sinden RR, Lyubchenko YL: Structure and dynamics of supercoil-stabilized DNA cruciforms. J Mol Biol 1998, 280: 61–72. 10.1006/jmbi.1998.1855View ArticlePubMedGoogle Scholar
- Shlyakhtenko LS, Potaman VN, Sinden RR, Gall AA, Lyubchenko YL: Structure and dynamics of three-way DNA junctions: atomic force microscopy studies. Nucleic Acids Res 2000, 28: 3472–3477. 10.1093/nar/28.18.3472PubMed CentralView ArticlePubMedGoogle Scholar
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