Figure 3From: The structure of Mycobacteria 2C-methyl-D-erythritol-2,4-cyclodiphosphate synthase, an essential enzyme, provides a platform for drug discoverySouthern blot of DispF complemented with pAPA3-ispDF (lanes 2–5) and WT (lane 1)- DNA was digested with Bam HI and probed with an upstream region of ispF. Expected size of WT band was 7.3 kb; DispF mutants were shown to only to possess the deletion band (3.1 kb), thus confirming the deletion of ispF.Back to article page