- Research article
- Open Access
Analysis of proteins with the 'hot dog' fold: Prediction of function and identification of catalytic residues of hypothetical proteins
© Pidugu et al; licensee BioMed Central Ltd. 2009
- Received: 25 November 2008
- Accepted: 28 May 2009
- Published: 28 May 2009
The hot dog fold has been found in more than sixty proteins since the first report of its existence about a decade ago. The fold appears to have a strong association with fatty acid biosynthesis, its regulation and metabolism, as the proteins with this fold are predominantly coenzyme A-binding enzymes with a variety of substrates located at their active sites.
We have analyzed the structural features and sequences of proteins having the hot dog fold. This study reveals that though the basic architecture of the fold is well conserved in these proteins, significant differences exist in their sequence, nature of substrate and oligomerization. Segments with certain conserved sequence motifs seem to play crucial structural and functional roles in various classes of these proteins.
The analysis led to predictions regarding the functional classification and identification of possible catalytic residues of a number of hot dog fold-containing hypothetical proteins whose structures were determined in high throughput structural genomics projects.
- Hypothetical Protein
- Catalytic Residue
- Bury Surface Area
- Central Helix
- Active Site Loop
It appears that the hot dog fold is designed for coenzyme A (CoA) binding in cellular processes involving fatty acids and related molecules. The binding site in FabA has been identified as a well-formed deep tunnel present at the subunit interface . Residues from both subunits contribute to the formation of the tunnel which is hydrophobic in nature except at the two catalytic residues His70 and Asp84. FabA, being a homodimer, consists of two tunnels related by a twofold symmetry. The active site is located slightly below the surface of the protein and the active residues at each site come from a different subunit.
Dillon and Bateman  have unified the large superfamily of hot dog fold proteins by an extensive analysis of their sequences. They have classified a large number of proteins from prokaryotes, archaea and eukaryotes of this superfamily into 17 subfamilies based on the clustering of sequences and available structural and biochemical information. Consensus sequence motifs were identified by them for each subfamily. Though all these proteins possess the hot dog fold, the inter-subfamily sequence similarities are very low (10–20%). They showed that the hot dog fold domain, though exists as a single entity of the coded proteins, also associates with other domains, suggesting the occurrence of domain fusion events involving this domain. It has been found to be associated with a second hot dog fold domain in tandem and also with various types of other domains such as LpxC (UDP-3-O-acyl N-acetylglucosamine deacetylase), AMP binding, acyl transferase, aldehyde dehydrogenase and a few more .
In the last two years, there has been an enormous increase in reports of the structures of hot dog fold containing proteins and the deposition of their coordinates in the Protein Data Bank. Currently, the PDB contains more than sixty non-redundant crystal structures of proteins with the hot dog fold, a majority of them being hypothetical proteins taken up by structural genomics programs, for which no other characterization has been reported until now (see Additional file 1). The availability of a reasonable number of crystal structures of hot dog fold proteins facilitated a more detailed comparative analysis across the subfamilies. Here, we attempt to group these hot dog fold proteins into their corresponding subfamilies and analyze the structure-function relationships of the subfamilies having representative crystal structures. This paper describes a detailed structural comparison of the hot dog fold proteins and their different modes of quaternary associations and functional diversity. This analysis made it possible to predict functional subfamilies and identify possible catalytic residues for a number of hypothetical proteins with known structures which should aid in further experimental verifications.
Diversity in quaternary association and function
An analysis of these proteins indicates the following general features: The hot dog fold domain does not exist as a single entity. It always forms a dimer (or a double hot dog) or higher oligomers. There are different modes of association of the basic building blocks in higher oligomers, but strict correlation between oligomeric state and function has not always been observed. Subfamilies with hot dog fold proteins of similar quaternary associations differ in their substrate specificities. On the other hand, proteins with different quaternary associations and highly divergent sequences can carryout the same reaction as in the case of 4-hydroxybenzoyl-CoA thioesterases. In all the crystal structures considered, the consensus sequence motifs identified earlier [2, 4] are localized at the active site and at the interface of the quaternary association indicating that the information about the substrate specificity and quaternary association are encoded in the sequence. Variations of the consensus sequence at the interface change the oligomeric state. The double hot dog domains are found to accommodate bulkier substrates and generally contain one active site per dimer instead of two. The structure-function relationships of subfamilies with the available representative crystal structures (see Additional file 1 and 2) are discussed here.
Subfamilies of the hot dog fold suprefamily of proteins
1. Dehydratases (FabA and FabZ)
The two subfamilies of dehydratases, FabA-like dehydratases and FabZ-like dehydratases have been well studied both biochemically and structurally. Both FabA and FabZ are isozymes catalyzing the third step of elongation in Type II fatty acid biosynthesis. FabZ is only a dehydratase while FabA has an additional isomerase activity. The biological oligomer of FabZ is a hexamer [5–7], a trimer of dimers where the active site loops are stabilized by the interactions at the hexameric interface. However, FabA is active as a dimer . Dimers of FabA and FabZ superpose with a rmsd of around 1.5 Å and the positions of the catalytic dyad (Glu-His in the case of FabZ and Asp-His in the case of FabA) are structurally conserved. The two cis peptides Tyr-Pro (FabZ)/Ala-Pro (FabA) in the N-terminal loop lining the active site tunnel and His-Phe harbouring the catalytic histidine are conserved in both the classes of enzymes. However, there is a subtle difference in the overall architecture of the active site tunnels, which was attributed to the additional isomerase activity of FabA . It was shown by domain-swapping experiments that the β-strand at the dimer interface and the loop connecting this β-strand to the next one are important for isomerase activity . In the case of FabA, consensus sequence motifs are localized to the loops that form the active site tunnel and the region responsible for the isomerase activity, while in the case of FabZ, the consensus sequence motifs are located on active site loops which are also involved in hexameric contacts. When a hexamer of FabA was generated by superimposing three dimers onto the three dimers of hexameric FabZ, severe steric clashes of less than 1 Å were observed at the dimer-dimer interfaces between the atoms of the loops containing the catalytic histidine and the loop involved in isomerase activity, thus indicating the neccessity of the dimeric state of FabA to function as a dehydratase/isomerase.
Each step of the FAS II pathway present in bacteria and plants is catalyzed by a distinct enzyme. In contrast to this, in the FAS I pathway of mammals and fungi, all the reactions are catalyzed by a large single multienzyme complex called the synthase. The dehydratase domain identified in each subunit of the homodimeric porcine fatty acid synthase structure (PDB code: 2CF2) was found to have the double hot dog fold flanked by FabD (malonyl-CoA/acyl-CoA-ACP transacylase) and FabI (NADPH-dependent β-enoyl reductase) domains. As the structure is available at a low resolution of 4.5 Å and the deposited coordinates contain only the Cα atoms of the fitted model, it is not included in further analysis presented in the paper.
This group of enzymes catalyzes the hydrolysis of the thioester bond in fatty acids bound to CoA or acyl carrier protein (ACP) . The thioesterases belong to different subfamilies of hot dog fold proteins as they differ in their substrate specificities and are also highly divergent in sequences. Eight of the seventeen subfamilies  of hot dog fold proteins are thioesterases and representative crystal structures are available for all of these thioesterase subfamilies (see Additional file 1).
a. Acyl-CoA thioesterases
b. TesB-like thioesterases
TesB-like thioesterases found mostly in bacteria and eukaryotes include the human peroxisomal enzyme that binds to the HIV Nef protein. The crystal structure of TesB, the medium chain acyl-CoA thioesterase II from E. coli (EcThII; PDB code: 1C8U) for the first time showed the structure of a double hot dog. The biologically active oligomeric state is a dimer of double hot dogs (DdhB) with the consensus sequence at the dimer interface and the active site. The dimer is formed by the back to back stacking of the 12 stranded β-sheets. The crystal structure revealed a novel catalytic mechanism wherein a hydrogen-bonded triad of Asp204, Thr228 and Gln278 are identified by site directed mutagenesis as catalytic residues and synergistically activate a water molecule for nucleophilic attack . The crystal structure of a putative peroxisomal thioesterase from yeast (PDB code: 1TBU) with the hot dog fold structure forms a tetramer of type TB and has only the first few consensus sequence motifs which do not include the catalytic triad.
c. YbgC-like thioesterases
The YbgC-like thioesterases, that belong to the tol-pal cluster, act on short chain aliphatic acyl-CoA as shown in the gamma-proteobacterium Haemophilus influenzae . This tol-pal cluster operon is well conserved in all gram-negative bacteria and is shown to be crucial for the maintenance of cell envelope integrity . The crystal structures of the hypothetical proteins with PDB codes: 1S5U (E. coli), 1Z54 (T. thermophilus), 2GF6 (S. solfataricus), 2HX5 (P. marinus) and 2EGJ (A. aeolicus) have a hot dog fold in the subunits and a consensus sequence of the YbgC-like subfamily. Further, a tetramer of the type TA is formed within the asymmetric unit or with the symmetry-related molecules, indicating that the tetramer might be the biologically active oligomer. Zhuang et al. (2002)  have shown in the case of the H. influenzae YbgC protein that the D18N mutant was not capable of hydrolyzing the short chain aliphatic acyl-CoA thioesters, thus suggesting a catalytic role for this aspartate residue. This residue present in the consensus sequence motif is conserved in all the proteins (see Additional file 2) mentioned above.
d. 3-hydroxyacyl-CoA dehydrogenase-associated thioesterases
3-hydroxyacyl-CoA dehydrogenase-associated thioesterases are specific to short chain fatty acids of fatty acid metabolism catalyzing the reduction of 3-hydroxyacyl-CoA to 3-oxoacyl-CoA . The crystal structure of a hypothetical protein from Pseudomonas putida (PDB code: 2HLJ) possesses the conserved sequence motif of the hydroxyacyl-CoA dehydrogenase subfamily. One hot dog subunit is present in the asymmetric unit and forms a dimer with a symmetry-related molecule with the consensus sequence motif present at the active site.
e. 4-Hydroxybenzoyl-CoA thioesterases
4-Hydroxybenzoyl-CoA thioesterases, encoded by the fcbC gene, are involved in the degradation of the environmental pollutant 4-chlorobenzoate to 4-hydroxybenzoate. The 4-chlorobenzoyl-CoA degradation pathway operons of certain Pseudomonas and Arthobacter bacterial strains show significant differences in gene order and sequences . Both the subfamilies (i) 4HBT-I (from Pseudomonas sp. strain CBS-3; PDB code: 1BVQ, 1LO7) and (ii) 4HBT-II (from Arthobacter sp. strain SU*; PDB code: 1Q4T) have been studied both biochemically and structurally. Though both the enzymes form tetramers, they have different modes of quaternary association, CoA binding sites and catalytic architecture. In the case of 4HBT-I, TA tetramers are formed, while 4HBT-II enzymes form TB tetramers. The structures of these two enzymes in complex with inhibitors revealed that the 4-hydroxy phenacyl moieties are oriented such that the thioester C = O can form a hydrogen bond with the amide nitrogen atoms of Tyr24 and Gly65 located at the N-terminus of the central helix in Pseudomonas (4HBT-I) and Arthobacter (4HBT-II), respectively. Due to the hydrogen bond and the polarity of the helix, the C = O group is polarized, making it susceptible to nucleophilic attack. Though the catalytic mechanism is similar, the positions of the carboxylate residues, Asp17 in 4HBT-I (1BVQ) and Glu73 in 4HBT-II (1Q4T), involved in catalysis are different. These two enzymes having the same fold and catalyzing the same reaction differ remarkably in their quaternary associations. In 4HBT-I the active site is formed at the dimer interface, while in 4HBT II three subunits contribute to the active site. A hypothetical protein (PDB code: 2OAF) has a consensus sequence motif of that of 4HBT-I and forms a TA tetramer. For 4HBT-II, the extra N-terminal motif, which is also one of the consensus motifs, is positioned before the central helix and prevents the formation of helix-to-helix packing observed in the quaternary association of 4HBT-I. The crystal structures of hypothetical proteins with PDB codes: 1O0I (H. influenzae), 1VH5 (E. coli) and 1VH9 (E. coli) have the consensus sequence of the 4HBT-II subfamily and a similar mode of quaternary association. In all these proteins, the catalytic machinery is both sequentially and structurally conserved within the subfamilies.
f. PaaI-Thioesterases specific to phenylacetic acid
g. Fat-Thioesterases specific to acyl-ACPs
Acyl-ACP thioesterases terminate the Type II fatty acid biosynthesis by hydrolysing the thioester bond between an acyl moiety and ACP . Two gene classes, fatA and fatB, exist in higher plants, and differ in sequence and substrate specificities [27, 28]. The Fat subfamily of hot dog fold proteins contains members from both FatA and FatB classes . The crystal structures of hypothetical proteins from B. thetaiotaomicron (PDB code: 2ESS) and L. plantarum (PDB code: 2OWN) possess tetramers formed by the dimers of double hot dogs with the central helices pointing inward (DdhA). This central helix contains the consensus sequence of the Fat subfamily. These proteins share a sequence identity of >20% with FatA and FatB of Arabidopsi s thaliana. Therefore, these two hypothetical proteins can be considered as members of the Fat subfamily.
3. (R)-specific enoyl-CoA hydratases or MaoC dehydratase-like subfamily
The consensus sequence motif for this subfamily has been identified by Qin et al. (2000) . The current analysis suggests that the overhanging segment containing this sequence motif can also be considered as a structural motif of this subfamily as it is present in all the three proteins (1IQ6, 1PN2 and 1S9C) for which crystal structures and biochemical characterization are available. Three hypothetical proteins, one from Mycobacterium tuberculosis (PDB code: 2BI0) and two from Archaeoglobus fulgidus (PDB codes: 1Q6W and 2B3M) can be classified into this subfamily. The first one is a trimer of double hot dog folds, the second one is a trimer of dimers and the third one is a dimer of hot dog folds indicating that the quaternary association is not conserved in this subfamily. Though all these proteins have hydratase activity, they differ in their substrate specificities. For example, recently cloned and characterized R-hydratases from the P. aureginosa PHA biosynthesis pathway, PhaJ1-PhaJ4 show a variation in activity based on the length of the fatty acyl chain of enoyl-CoAs . PhaJ1 can act only on short chain enoyl-CoAs (C4–C6), while the other three can act on longer (C8–C12) enoyl-CoAs. The consensus sequence motif of the hydratase 2 domain  though present in this subclass of enzymes shows variation (see Additional file 2), reflecting the differences in quaternary association and substrate specificities. However, all the proteins of this subfamily share a sequence identity of around 15–40% and the catalytic dyad Asp31 & His36 is highly conserved both sequentially and structurally.
The crystal structures of FAS I synthase of yeast (PDB code: 2UV8) and Thermomyces lanuginosus (PDB codes: 2UV9 and 2UVA) are available at 3.1 Ǻ resolution. In contrast to the mammalian homodimeric enzymes, these two exist as α6β6 dodecamers. The dehydratase domain located on the β subunit has a unique triple hot dog fold. The first and the third hot dog domains associate as pseudodimers and are structurally similar to eukaryotic hydrates 2 enzymes having the double hot dog fold with the catalytic residues in the overhanging segment of the C-terminal domain. The second hot dog domain is inserted in the long loop connecting the first and the third hot dog domains in the same way the domains in the double hot dog structures are connected. The central helix of this domain is much shorter and has a different orientation compared to that in the typical hot dog domain described here. The yeast enzyme, which is well refined, has been included in the present analysis.
4. YbaW subfamily
Structural genomics programs have shown that the crystal structures of conserved hypothetical protein YbaW (PDB codes: 1NJK from E. coli, 2ALI from P. aeruginosa, 2AV9 from P. aeruginosa, 2CYE from T. thermophilus, 2FUJ from X. campestris , 2NUJ from Jannaschia sp. CCS1, 2OIW from B. stearothermophilus) with the hot dog fold form tetramers of type TA similar to that of 4HBT-I. However, the sequences of both the subfamilies are quite different. Asp20 has been identified as the catalytic residue in the case of YbaW from X. campestris (PDB code: 2FUJ) which is conserved among all the other hypothetical proteins of this subfamily. This residue occupies the same position as His133 of Plasmodium falciparum FabZ enzyme (PDB code: 1Z6B). We have also seen a Glu/Asp residue in the consensus sequence (see Additional file 2) similar to Glu147 of PfFabZ.
5. FapR subfamily
The crystal structures of three hypothetical proteins (PDB codes: 1T82 from S. oneidensis, 1SH8 from P. aeruginosa, and 1YOC from P. aeruginosa) were found to be homodimers. These proteins share 27%, 38% and 34% sequence identity, respectively with the putative acetyltransferase YiiD from E. coli. However, the consensus sequence motif is not well conserved in these proteins. A glutamate residue in 1SH8 and 1YOC (see Additional file 2) has been identified as the catalytic residue by structural superpositions. However, the corresponding one is a threonine in 1T82.
Other proteins with the hot dog fold
Other three subfamilies namely, CBS-associated, MSCP and AMP binding subfamilies  do not have representative crystal structures. On the other hand, two hypothetical proteins from T. thermophilus (PDB code: 2CWZ) and T. maritime (PDB code: 2Q78) possess the hot dog fold, but it was not possible to assign a subfamily to these proteins as they do not have any of the consensus sequence motifs of the known subfamilies. These proteins form a dimer of hot dogs. In addition, they have a two stranded β-sheet towards the N-terminus and a long helix towards the C-terminus. Phylogenetic analysis also shows that these proteins group together but do not cluster with any of the subfamilies. This might be a representative member of an unexplored subfamily of hot dog fold proteins. The yeast protein, a member of Phenazine biosynthesis, PhzF (PDB code: 1YM5) has a kinked double hot dog with a similar domain structure but a domain association different from that described so far. Each hot dog subunit contains nine β-strands around the central α-helix which is shorter in length compared to those discussed so far.
The structure of Rv0098 from M. tuberculosis forms a new head-to-tail hexameric association (H3) made up of trimers of dimers of hot dog fold domains (PDB code: 2PFC). This enzyme has been identified as a long chain acyl-CoA thioesterase with a catalytic site different from the known thioesterases with three residues Tyr33, Tyr66 and Asn74 stabilizing the transition state of the substrate.
Structure-based phylogenetic analysis
CoA binding and catalytic sites
Comparison of active site residues
Additional Thr & Gln
TB & D
D, DdhA, Trdh, H2
Asp in a slightly different location
Highly divergent sequences lead to similar structures
The present analysis shows correlations between the oligomerization, function and sequence of proteins with the hot dog fold (see Additional file 1, 2 and Figure 9). At the subunit level, the proteins possess highly divergent sequences but adopt a similar fold. A detailed comparison of these proteins revealed conserved hydrophobic interactions between the residues from the central helix and the various parts of the curved β-sheet. Though the residues involved in these interactions are from different parts of the sequence in different proteins, the interactions are structurally conserved and hence form the driving force for the formation of the hot dog fold.
Ligand and pH induced Structural Changes
Two of the 64 proteins discussed in this analysis show structural changes upon ligand binding. In the case of T. thermophilus PaaI (PDB code: 1J1Y) protein a small rotaton of 2° in one of the dimers of the TB tetramer results in negative co-operativity and an asymmetric induced fit mechanism inducing half of the sites reactivity. In the case of B. subtilis FapR (PDB code: 2F3X), upon ligand binding, three flexible loops become ordered, a substrate binding tunnel is formed leading to conformational changes in the helix-turn-helix motif resulting in the impairment of DNA binding.
As a extension of the analysis of the hot dog fold present in the structure of the enzyme FabZ of P. falciparum that we determined , we probed the nature of this fold present in other proteins as well. Our analysis led to the identification of the probable functions of the hypothetical proteins containing the hot dog fold through detailed structural comparisons and identification of sequence motifs. Our analysis also demonstrates that quaternary association and function are related in certain cases. The consensus sequence motifs localized to the interface of quaternary structure and active site direct the mode of association and substrate specificity. Different modes of quaternary association were observed in the MaoC subfamily where the consensus sequence motif at the interface varies considerably. Double hot dog domains might have evolved by gene duplication to accommodate bulky substrates and to regulate activity by the asymmetric induced fit mechanism. It appears to be a case of directed divergent evolution where sequences changed substantially to design various types of quaternary association to carry out different functions with the same fold.
Functional annotation of hot dog fold proteins
All non-redundant crystal structures containing the hot dog fold in the PDB were identified using the DALI server hosted by the EMBL-EBI  using a dimer of FabZ from P. falciparum (PDB code: 1Z6B) as a search model. The sequence of one representative structure from each subfamily obtained from the DALI search was submitted to the Blastp server of NCBI  and a search for homologues in the PDB was carried out which resulted in the identification of all the structures with the hot dog fold within the subfamilies. A total of 64 non-redundant crystal structures were considered for the present analysis. Out of these structures, more than forty are from various structural genomics programs and have not been biochemically characterized so far. We attempted to assign functions to these proteins based on sequence and structural analysis. These crystal structures were grouped into respective subfamilies according to the presence of consensus sequence motifs  and available biochemical information. For the structures where the consensus sequences were not strictly followed, homologous proteins were picked up from the Swissprot database using the BLAST server. The consensus sequence motifs could be readily identified in these homologous proteins and these were used to assign the function. The crystal structures were visually examined in PyMOL [DeLano Scientific LLC] to identify the repeating structural unit. The possible quaternary association was identified by displaying the symmetry-related molecules and was further confirmed by examining the buried surface areas using the server MSDpisa . For the hypothetical proteins classified into structurally and functionally characterized subfamilies, the functional annotation was confirmed by the similarities in the mode of quaternary association and structural conservation of the active site architecture. Pairwise structural superposition of the monomers and dimers was carried out using DaliLite. These results are used to calculate the structural distance measure (SDM) [48, 49]. SDM values are calculated based on structural superposition and thus provide a better model for the evolutionary relation of proteins with very low sequence identities. MEGA  is used to generate the final phylogenetic tree using the distance matrix of the SDM values.
We thank N. Srinivasan and his group members for useful discussions and help with the phylogenetic analysis. The work is supported by a grant from the Department of Biotechnology (DBT), Government of India, to NS and KS.
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