- Research article
- Open Access
A lack of peptide binding and decreased thermostability suggests that the CASKIN2 scaffolding protein SH3 domain may be vestigial
BMC Structural Biologyvolume 16, Article number: 14 (2016)
CASKIN2 is a neuronal signaling scaffolding protein comprised of multiple ankyrin repeats, two SAM domains, and one SH3 domain. The CASKIN2 SH3 domain for an NMR structural determination because its peptide-binding cleft appeared to deviate from the repertoire of aromatic enriched amino acids that typically bind polyproline-rich sequences.
The structure demonstrated that two non-canonical basic amino acids (K290/R319) in the binding cleft were accommodated well in the SH3 fold. An K290Y/R319W double mutant restoring the typical aromatic amino acids found in the binding cleft resulted in a 20 °C relative increase in the thermal stability. Considering the reduced stability, we speculated that the CASKIN2 SH3 could be a nonfunctional remnant in this scaffolding protein.
While the NMR structure demonstrates that the CASKIN2 SH3 domain is folded, its cleft has suffered two substitutions that prevent it from binding typical polyproline ligands. This observation led us to additionally survey and describe other SH3 domains in the Protein Data Bank that may have similarly lost their ability to promote protein-protein interactions.
A pair of neuronal scaffolding proteins, represented in humans by CASKIN1 and CASKIN2, participate in signaling pathways involved in axon guidance and the creation of neuromuscular junctions. While mammals possess two CASKIN homologs, Drosophila only possesses one form (Ckn) that differs in domain composition and C. elegans has no homolog at all leading to the idea that the general role of CASKINs may be to provide more complex signaling outcomes in higher organisms by recruiting additional sets of protein partners . Typical for many scaffolding proteins [2, 3], CASKIN1 and CASKIN2 are composed entirely of protein-protein interaction domains including six ankyrin repeats, an SH3 domain and tandem SAM domain (Fig. 1a). The C-terminal half of the protein, spanning over 600 residues and only partially conserved, is characterized by a proline-rich segment of unknown function .
CASKIN1, and CASKIN2 by homology, were named by their ability to serve as ligands of the MAGUK protein, CASK, a prominent signaling protein linked to calcium mediated signaling events, actin microfilament assembly, and synaptic communication through the neurexin cell surface receptor [4, 5]. Later structural studies of CASKIN1 revealed that the CASK interaction domain (CID), shared with X11/Mint and located in a region between the SH3 and SAM domains, was not present in CASKIN2. Thus, the namesake of CASKIN2 is misleading because it is unable to bind the calmodulin kinase domain of CASK . This distinction lead us to speculate that despite their organizational similarity, CASKIN1 and CASKIN2 may have diverged with respect to their scaffolding functions in neurons .
Owing to the prominence of CASK in the development of neurological disease [8, 9], research to date has concentrated on CASKIN1 [10–12]. In this report, we have concentrated on the CASKIN2 SH3 domain to explore the question of whether functional differences between CASKIN1 and CASKIN2 extend to the structural level. Building upon the NMR solution structure of the CASKIN2 SH3 domain, we demonstrate that the typical binding cleft has two hydrophobic amino acid substitutions that do not affect the overall fold, but do affect thermostability and the ability to interact with peptides. These observations led us to consider that the CASKIN2 SH3 domain was non-functional. When hydrophobic amino acids were reintroduced, thermostability increased and high affinity binding was restored towards a peptide ligand of one of CASKIN2’s most homologous family members.
Results and discussion
Domain organization and sequence similarity
The CASKIN1 and CASKIN2 SH3 domains are located at the same position within each protein and are approximately 60 % similar (Fig. 1b). Following the SH3 domain, CASKIN1 and CASKIN2 diverge in sequence, the notable differences being the lack of a CASK interaction domain (CID) in CASKIN2, as well as the length and composition of a carboxy terminal proline-rich region. Thus, depending on the perspective, CASKIN1 and CASKIN2 are simultaneously similar and different, and therefore warrant some caution when making functional assumptions about one protein in the absence of knowledge from the other.
Structure of the CASKIN2 SH3 domain
We began this study with a NMR solution structure determination of the CASKIN2 SH3 domain to provide a high-resolution framework for exploring how the SH3 domain may interact with ligands and react to post-translational modifications. Backbone atom precision for the ensemble of structures was 0.60 ± 0.14 Å, consistent with the number of experimental observations used in the structure calculation. Complete statistics pertaining to the experimental observations and structural quality are presented in Table 1. From the ensemble of the lowest energy structures, the RT and N-Src loops presented a high degree of disorder (Fig. 1c), presumably due to the absence of a ligand. Experimentally, the decrease in backbone atom precision is manifested by missing backbone assignments (D297, S327-D330, and R333) and short-range NOE observations (depicted as dots above the sequence in Fig. 1). A survey of the PDB for similar structures revealed the SH3 domains from human KIAA17833 and yeast NBP2 (both from structural proteomics studies) along with the well characterized SH3 domain from STAM2  (Table 2).
The CASKIN2 SH3 domain peptide binding cleft
A typical SH3 domain presents a binding cleft comprised of six, nearly linearly arranged, hydrophobic amino acids. As shown in Fig. 1d, the six amino acids in CASKIN2 are K290, I341, F292, P338, R319, and Y336. Notably, there is strong deviation at the first and fifth positions, denoted by K290 and R319. Since the side chains of the SH3 domain binding cleft are surface exposed, K290 and R319 do not affect the protein fold, and presumably, this is also the case for CASKIN1. Given that the binding cleft deviates at two positions from the typical set of amino acids observed in PxxP type SH3 domains like Abl kinase  and RxxK type SH3 domains like STAM2 , we hypothesized that the CASKIN2 SH3 domain might bind an unconventional peptide ligand. To answer this question, we performed four successive rounds of panning a 6xHis−-tagged CASKIN2 SH3 domain against a commercial bacteriophage 12-mer peptide display library (PHD-12; Novagen). A comparison of eight peptides from the last round of panning revealed a Pxx[L/M/W] motif in many of the candidates [presented in Additional file 1]. However, upon testing this motif with two chemically synthesized peptides and one reversed peptide as a control, we did not observe any binding in NMR based titrations suggesting that the library may have identified a very weak interaction. Since the phage display method failed to identify a ligand for the CASKIN2 SH3 domain, it led us to consider that the CASKIN2 SH3 domain might be a non-functional remnant of the protein.
Figure 1e presents a comparison of high resolution SH3 domain structures from the Protein Data Bank with an emphasis on representatives having peptide binding clefts that deviate from the usual complement of aliphatic and aromatic amino acids, and consequently, have no ligands reported for them. The Abl SH3 domain leads a set of sequences in Fig. 1e as a point of reference and prototype for the typical array of aromatic and aliphatic amino acids that populate the six positions of the binding cleft. Next to Abl in the presentation is the RUN/TBC1 domain containing protein-3 SH3 domain (PDB: 2YUO) that swaps an aromatic/aliphatic pair at positions 5 and 6 of the cleft with a compensatory aliphatic/aromatic pair. A survey of the remaining representatives in the figure demonstrate that it is possible for each of the six binding cleft positions to tolerate a hydrophilic or charged amino acid substitution. The Crk-II SH3(2) domain represents the most extreme case with substitutions at positions 1, 2, 5 and 6. Loss of ligand binding in the Crk-II SH3(2) domain in the context of the full adaptor protein is consistent with a stabilizing function rather than a regulatory function in this domain .
Substitution mutagenesis of the CASKIN2 peptide binding cleft
A CASKIN2 SH3 domain double mutant (K290Y, R319W) that we termed SH3-2x, was expressed to reintroduce a full complement of aromatic amino acids in the peptide binding cleft. Using circular dichroism (CD) spectroscopy, the SH3-2x protein demonstrated a 20 °C increase in its thermal denaturation midpoint (Table 3). This observation suggests that aromatics in these two positions contribute to the stability of the SH3 fold. Given the high degree of structural similarity to the STAM2 SH3 domain in complex with a UBPY peptide , we introduced four more substitutions into the SH3-2x framework to produce a full mimic of the STAM2 interaction in the CASKIN SH3 domain. Overall, this new mutant termed SH3-6x, contains two substitutions to reintroduce aromatics (K290Y, R319W), two minor substitutions that maintain hydrophobicity (Y336L, I341F) and two substitutions in the RT-loop (H296E, A300E) that provide important complementary ionic contacts to the UBPY peptide ligand in STAM2. Since the SH3-2x mutant was a subset of the SH3-6x mutant, the expressed protein had a similar thermal denaturation midpoint of 70 °C (Table 3). When tested against a Class-III UBPY peptide containing an RxxKP motif, high affinity binding (0.46 ± 0.11 μM) was observed (Fig. 2) in the same order of magnitude as another Class-III SH3 domain GADS with an SLP-76 peptide ligand . As expected, the wild type CASKIN2 SH3 domain had an over 100 fold less affinity for the UBPY peptide. While ultralow affinity SH3 domains with biological relevance have been reported, there is no evidence to suggest if CASKIN2 is among this group [18, 19].
The NMR structure of the CASKIN2 SH3 domain demonstrates a variant ligand binding cleft populated with charged amino acids. Based on this unique feature, we hypothesized that the CASKIN2 SH3 domain may bind a ligand that does not belong to any of the canonical, mainly proline-rich sequences that SH3 domains typically bind. Since a phage display failed to identify a ligand for the CASKIN2 SH3 domain, we concluded that it may indeed be non-functional. Increased thermal stability and ligand binding could be engineered back into the CASKIN2 SH3 domain sequence with relatively few amino acid substitutions. This results presented in the report emphasize the considerable versatility that SH3 domains, and signaling adaptor proteins, in general, have at their disposal.
Cloning, expression and protein purification
The human CASKIN2 SH3 domain (aa. 284–348; Uniprot Q8WXE0) was amplified by PCR from a human cDNA and inserted into the BamHI and XhoI restriction sites of pGEX4T2 (GE Life Sciences) followed by transformation into E. coli BL21:DE3 to produce a glutathione S-transferase (GST) tagged fusion protein with intervening thrombin protease cleavage site. Two CASKIN2 SH3 domain mutants, termed 2x and 6x, contained two aromatic substitutions to reconstitute a typical hydrophobic binding cleft and six substitutions mimicking the canonical binding cleft of the STAM2 SH3 domain. These mutants were made using Quikchange method (Agilent). All constructs were verified by sequencing at the York University Core Facility. Isotopic labeling of wild type GST-CASKIN2 SH3 domain and mutants for NMR spectroscopy was achieved by performing a 1.5 L fermentation in a minimal medium containing 1 g of 15NH4Cl as the sole nitrogen source and/or 3 g of 13C-glucose as the sole carbon source. The cell pellet was suspended in T300 buffer (20 mM Tris-HCl, 300 mM NaCl, 0.05 % NaN3) and lysed by French press. Purification of the GST-CASKIN2 SH3 protein was achieved by glutathione affinity chromatography (GE Biosciences). After an 8-h digestion with activated human thrombin (Sigma-Aldrich), the SH3 domain was resolved from the GST carrier protein by gel filtration chromatography (Sephacryl-100, HiLoad 16/60; GE Life Sciences). The final buffer for all analyses was phosphate buffered saline (PBS; 20 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.05 % (w/v) sodium azide.
Far UV spectra (190–260 nm) of CASKIN SH3 proteins at 20 μM in PBS were obtained on a Jasco J-810 spectropolarimeter and processed with Spectra Analysis 1.54.04 software. A midpoint thermal denaturation curve (Tm) was generated by monitoring the ellipticity signal at 220 nm as the temperature was ramped from 20 to 90 °C at a rate of 1 °C/min.
A solution of peptide YTPMVRENKPP at > 90 % purity (Canpeptide; Montreal, QC) corresponding to the UBPY ligand in the STAM2 SH3 domain structure (PDB: 1UJ0) was titrated into 1 μM wild type CASKIN2 SH3 domain, a 6x CASKIN-SH3 domain mutant, or a reference cell containing buffer only (10 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.05 % sodium azide). Upon excitation at 280 nm, intrinsic fluorescence emission was measured at 340 nm corresponding to one partially buried tryptophan in the CASKIN2 SH3 domain. Each measurement was made three times and averaged.
NMR spectroscopy and structure determination
All experiments were performed on a uniformly 13C,15N labeled sample of the CASKIN2 SH3 domain sample at 0.6 mM plus 10 % D2O. A conventional heteronuclear, triple-resonance strategy was employed with all experiments being acquired on an Agilent 600 MHz spectrometer equipped with a 5 mm cryoprobe. Backbone directed experiments : HNCACB and CBCAcoNH (15Nsw = 1450 Hz, 15Npts = 28; 13Csw, = 11309 Hz, 13Cpts = 40), HNCO and HNcaCO (15Nsw = 1450 Hz, 15Npts = 28; 13Csw, = 2262 Hz, 13Cpts = 28). Side chain directed experiments: HccoNH (15Nsw = 1450 Hz, 15Npts = 28; 1Hsw, = 6596 Hz, 1Hpts = 80), hCcoNH (15Nsw = 1450 Hz, 15Npts = 28; 13Csw, = 11309 Hz, 13Cpts = 28), HCCH-TOCSY (13Csw, = 11309 Hz, 13Cpts = 28), aromatic HBcbcgCD and HBcbcgcdCE (13Csw, = 4524 Hz, 13Cpts = 32). Distance restrains were measures from peak volumes from a 15N-edited NOESY (15Nsw = 1450 Hz, 15Npts = 28; 1Hsw, = 6596 Hz, 1Hpts = 80, 100 ms mixing time) and 13C-edited aliphatic (1Hsw, = 6596 Hz, 1Hpts = 80, 13Csw, = 2800 Hz, 13Cpts = 36, 100 ms mixing time) and aromatic 3D-NOESYs aliphatic (1Hsw, = 6596 Hz, 1Hpts = 80, 13Csw, = 2800 Hz, 13Cpts = 24, 100 ms mixing time). Datasets were processed with NMRpipe  and interpreted with NMRView . Distance restraints were obtained using CYANA. Backbone torsion angles were predicted from backbone chemical shift data with TALOS+ . From an initial set of 500 structures calculated with CYANA 2.0 , the top 25 structures were selected with no NOE violations > 0.3 Å and no torsion angle violations < 5°. This ensemble was then subjected to additional refinement in explicit solvent  with XPLOR-NIH 2.30  and 15 final lowest energy structures were selected for deposition to the Protein Data Bank.
Calcium/calmodulin-dependent serine protein kinase
CASK interacting protein-2
CASK interaction domain
Nuclear magnetic resonance
Protein data bank
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This research was funded by an operating grant (MOP-81250) from the Canadian Institutes for Health Research to LWD.
Availability of data and materials
Chemical shifts and coordinates of the wild type CASKIN2 SH3 domain were deposited in the BioMagResBank and Protein Data Bank with accession numbers 19178 and 2KE9.
JJK and LWD performed experiments and analyzed the data. LWD wrote the manuscript. JJK and LWD approved the manuscript.
The authors declare they have no competing interests.
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Application of phage display to identify a potential ligand for the CASKIN2 SH3 domain. (PDF 86 kb)