Functional insights from proteome-wide structural modeling of Treponema pallidum subspecies pallidum, the causative agent of syphilis
BMC Structural Biology volume 18, Article number: 7 (2018)
Syphilis continues to be a major global health threat with 11 million new infections each year, and a global burden of 36 million cases. The causative agent of syphilis, Treponema pallidum subspecies pallidum, is a highly virulent bacterium, however the molecular mechanisms underlying T. pallidum pathogenesis remain to be definitively identified. This is due to the fact that T. pallidum is currently uncultivatable, inherently fragile and thus difficult to work with, and phylogenetically distinct with no conventional virulence factor homologs found in other pathogens. In fact, approximately 30% of its predicted protein-coding genes have no known orthologs or assigned functions. Here we employed a structural bioinformatics approach using Phyre2-based tertiary structure modeling to improve our understanding of T. pallidum protein function on a proteome-wide scale.
Phyre2-based tertiary structure modeling generated high-confidence predictions for 80% of the T. pallidum proteome (780/978 predicted proteins). Tertiary structure modeling also inferred the same function as primary structure-based annotations from genome sequencing pipelines for 525/605 proteins (87%), which represents 54% (525/978) of all T. pallidum proteins. Of the 175 T. pallidum proteins modeled with high confidence that were not assigned functions in the previously annotated published proteome, 167 (95%) were able to be assigned predicted functions. Twenty-one of the 175 hypothetical proteins modeled with high confidence were also predicted to exhibit significant structural similarity with proteins experimentally confirmed to be required for virulence in other pathogens.
Phyre2-based structural modeling is a powerful bioinformatics tool that has provided insight into the potential structure and function of the majority of T. pallidum proteins and helped validate the primary structure-based annotation of more than 50% of all T. pallidum proteins with high confidence. This work represents the first T. pallidum proteome-wide structural modeling study and is one of few studies to apply this approach for the functional annotation of a whole proteome.
The medically important spirochete bacterium Treponema pallidum subspecies pallidum is the causative agent of syphilis. Syphilis is a chronic, multi-stage, systemic disease with three major modes of transmission; via sexual contact, exposure to an infectious lesion, and in utero. Although syphilis can be readily treated with penicillin, approximately 11 million new infections occur each year, contributing to a global burden estimated to comprise 36 million cases . Congenital syphilis, which results in spontaneous abortion, stillbirth, postpartum death, or newborn malformations, is also a major global health issue with an estimated 1.4 million pregnant women infected with syphilis each year . In addition, it has been well established that symptomatic syphilis infection increases the risk of HIV transmission and acquisition two- to five-fold . The global public health threat posed by syphilis emphasizes the need to gain a better understanding of the molecular mechanisms underlying T. pallidum pathogenesis, and in particular, the identification of suitable protein targets for vaccine design and diagnostic test applications.
Treponema pallidum is a highly virulent bacterium and is one of the most invasive pathogens known. Following infection, it rapidly invades the host tissue barrier and disseminates to distant tissues and organs via the circulatory system [4,5,6]. It is also one of the few pathogens capable of traversing both the placental and blood-brain barriers . Although 19 years have passed since the publication of the first T. pallidum complete genome sequence , virulence factors remain to be definitively identified. This is primarily due to four reasons. First, T. pallidum is an obligate human pathogen that is unable to be cultured continuously in vitro or genetically manipulated. The inability to culture T. pallidum is consistent with its reduced genome, which is comprised of 978 predicted protein-coding genes , and the corresponding absence of key biosynthetic pathways. Second, the bacterium is inherently fragile due to the presence of a unique outer membrane ultrastructure with few integral outer membrane proteins and an unusually thin peptidoglycan layer that is more distal to the outer membrane compared to conventional Gram-negative bacteria [9, 10], which renders laboratory manipulation challenging. Third, T. pallidum is a phylogenetically distinct bacterium, highlighted by the fact that almost 30% of predicted protein-coding genes still have no known orthologs or assigned functions [7, 8]. Finally, genome sequencing of T. pallidum identified very few conventional virulence factor candidates [7, 8].
Protein structure determination informs protein function and thus can be used to overcome some of the knowledge acquisition roadblocks stemming from experimental limitations associated with T. pallidum research. The three-dimensional structure of a protein determines its function, and protein structure is oftentimes more conserved than protein sequence . Hence, the solving of high-resolution protein structures has the potential to provide insight into the molecular mechanisms that mediate T. pallidum pathogenesis through delineation of structure-function relationships. However, to date only 18 unique protein structures from the 978 T. pallidum predicted protein-coding genes have been determined and deposited in the Research Collaboratory for Structural Bioinformatics Protein Data Bank [12, 13], representing only 1.8% of the proteome [14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31]. The analysis of whole proteome sequences using computational methodologies for protein tertiary structure modeling provides an alternative structural bioinformatics-based approach for improving our understanding of potential protein functions on a proteome-wide scale. Phyre2  is a widely-used protein tertiary structure modeling server that consistently ranks among the top-scoring protein tertiary modeling servers in critical assessment of protein structure prediction (CASP) trials [33, 34]. In addition, unlike other molecular modeling servers, Phyre2 is capable of rapidly modeling multiple proteins which makes it particularly well suited for analyzing whole proteomes. However, to date Phyre2 has only been employed for the structural and functional annotation of a limited number of whole proteomes [35,36,37].
In the current study, we used a Phyre2-based structural bioinformatics approach for molecular modeling and functional inference of the whole T. pallidum proteome. This methodology generated high confidence tertiary structure model predictions that provide novel insight into the structure and function of T. pallidum proteins with no known orthologs or assigned functions that may play important roles in the unique structural and virulence properties of T. pallidum. Furthermore, we found a high level of protein functional annotation agreement between our molecular modeling-based approach and genome sequencing pipelines that have relied primarily on amino acid sequence homology comparisons. These findings provide support for the effectiveness of structural modeling as a viable approach for predicting protein function on a proteome-wide scale, and suggest it may also serve as a valuable complementary tool in genome annotation pipelines. Finally, our structural bioinformatics approach also identified several potential T. pallidum virulence factors that represent promising targets for future experimental studies.
Treponema pallidum subspecies pallidum genome sequence
The genome of T. pallidum subspecies pallidum (Nichols) was recently re-sequenced, which resulted in improved sequence accuracy and functional annotation . This sequencing project used next-generation sequencing techniques which resulted in the identification of 978 predicted protein-coding genes. In the present study, we used the re-sequenced T. pallidum (Nichols) genome sequence (GenBank Accession Number CP004010.2)  for all tertiary structure modeling and analyses.
Whole proteome modeling of T. pallidum using Phyre2
Phyre2 is a web-based bioinformatics server for the prediction of protein structure, based on the principles of homology-based modeling [32, 38]. For each protein sequence to be modeled, there are four stages. The first stage involves the identification of homologous sequences and the generation of a multiple-sequence alignment (MSA). This is achieved by using the sequence alignment search tool HHBlits  to scan the query protein sequence against a non-redundant database which generates the protein’s sequence profile. PSIPRED  uses the MSA to predict secondary structure, and both the MSA profile and secondary structure predictions are then combined into a query hidden Markov model (HMM). In stage two, the query protein HMM is scanned against a fold library comprised of HMMs of known structure using the alignment algorithm, HHSearch . The highest scoring alignments are used to generate three-dimensional backbone models. Stages three and four involve modeling of loops and fitting of side-chains using the R3 protocol  to generate the final three-dimensional structural model, respectively.
The flow diagram shown in Fig. 1 outlines all major steps that comprised the Phyre2-based tertiary structure modeling pipeline of the T. pallidum proteome. In order to predict the three-dimensional structure of all T. pallidum proteins, and as the first step in gaining functional insights from proteome-wide structural modeling of T. pallidum, amino acid sequences corresponding to the 978 protein-coding genes identified by the recent re-sequencing of T. pallidum  were submitted in “batch mode” (maximum of 100 sequences per submission) to the protein fold recognition server, Phyre2  (Fig. 1a). For each of the 978 T. pallidum protein sequences, the 20 top-ranked template structural matches were obtained, corresponding to 19,560 templates with associated predicted models (Fig. 1b). Ranking is based on the number of amino acid residues that were aligned and the quality of the alignment. Alignment quality is based on the level of secondary structure similarities between the query sequence and template, the amount of insertions and deletions between the two sequences, and the similarity of amino acid probability distributions at each position of the query and templates sequences . Detailed template information relating to each ranked model was also obtained. These included a text description of the protein template including PDB descriptions (classification, header/molecule, and structure title fields) and the template PDB code. A confidence score (probability that the protein query/template match is a true homology), alignment coverage (coverage of the query protein sequence within the aligned region), and sequence identity (amino acid identity between the query and template protein sequences within the aligned region) were also obtained (all range from 0 to 100%). All coordinate files for each model in PDB format were downloaded for visualization and manipulation of each predicted model in the protein structure viewing/modeling program, UCSF Chimera [43, 44], and the corresponding Phyre2 batch mode results were incorporated into Microsoft Excel spreadsheets for data analyses (for raw unedited Phyre2 data corresponding to 19,560 templates used for whole proteome modeling, refer to Additional file 1: Table S1 [part 1]).
Identification of predicted T. pallidum protein tertiary structures modeled with high confidence
A structural model and template match with a confidence score of 90% or greater is considered highly reliable. This indicates the query protein is very likely to adopt the overall predicted fold and that the protein core is modeled at high accuracy (2–4 Å root-mean-square deviation from the true protein structure) . Therefore, for our analyses we set the minimum cut-off for confidence scores at equal to or greater than 90% (Fig. 1b).
Rationale for alignment coverage minimum cut-off value
The average T. pallidum protein size is approximately 340 amino acids . However, several structures of protein functional domains from other organisms as small as 30–40 amino acids have been solved and deposited in the RCSB PDB. Thus, in order to further increase the overall confidence in the modeling without omitting small potential functional domains (~ 30–40 amino acids), we set the minimum cut-off value for alignment coverage at 10% or greater (Fig. 1b). Sequence identity was not taken into account to increase model confidence at the whole proteome-modeling stage of the analyses. This was due to the fact that T. pallidum is genetically distinct from all other bacteria, including other treponemal species, and this study was focused on using tertiary structure prediction to decipher potential protein function in the absence of high amino acid sequence homology.
Method validation: Determining the level of correlation between T. pallidum genome sequence annotation and Phyre2-based annotation
To determine how well our Phyre2 analysis correlates with the genome sequence functional annotation, the predicted functions (derived from the model templates) of T. pallidum proteins modeled with high confidence (all template matches for each protein with ≥90% confidence and ≥ 10% alignment coverage) in the current study were compared with functional annotations derived from genome sequencing  (Fig. 1c). This analysis compared the protein functions inferred from Phyre2 modeling using (1) only the highest confidence tertiary structure model template function (proteins were assigned a function based solely on the single top-ranking template) and (2) all confident tertiary model protein template functions from the top 20 ranking models (proteins were assigned a function based on all template matches for each protein that generated models with ≥90% confidence and ≥ 10% alignment coverage). Proteins were categorized as having either the same function as the published genome annotations, related functions (same PDB functional group classification and/or related PDB molecule/template function), or alternative functions (including unknown functions).
Functional annotation of uncharacterized T. pallidum proteins
To gain insight into the potential function of uncharacterized proteins, we analyzed the functions of the 20 top-ranked template structural matches that Phyre2 used for modeling each protein with confidence and alignment cut-off values as described above (Fig. 1d).
Distribution of structure prediction confidence and coverage scores from T. pallidum proteome-wide structural modeling
The first step in our T. pallidum whole proteome modeling pipeline was to identify all proteins modeled by Phyre2 that have confidence scores of at least 90% (Fig. 1a and b). Out of 978 T. pallidum protein sequences submitted, 782 (80%) were modeled with a confidence score equal to or greater than our minimum cut-off value (Additional file 1: Table S1 and Fig. 2a). Of the 196 T. pallidum proteins that were modeled with confidence scores below our minimum cut-off value, 150 (77%) were annotated in the published T. pallidum proteome  as “hypothetical” or “putative membrane proteins” (Additional file 2: Table S2). The remaining 46 (23%) low-confidence models had functional annotations in the published T. pallidum proteome based on amino acid sequence homology (Additional file 3: Table S3). When we analyzed the physicochemical properties of the 46 proteins with low confidence models, wide value ranges were observed for each property with values similar to those previously reported for all T. pallidum proteins  (Additional file 3: Table S3). Nine of the 46 low-confidence models are annotated in the published proteome  as flagellar proteins, three are annotated as type 3 secretion system (T3SS)/injectisome proteins, eight are annotated as transport proteins, 16 are annotated with miscellaneous functions, and 10 belong to the Treponema pallidum repeat (Tpr) protein family  (Additional file 3: Table S3). Subcellular localization analyses predicted approximately 50% of the proteins to be localized to the inner membrane with 27–35 also predicted to contain transmembrane helices (Additional file 3: Table S3), which is indicative of an inner membrane locale.
To increase confidence in our modeling pipeline, we set the minimum alignment coverage cut-off value at 10% or greater. A total of 780/782 T. pallidum proteins that were modeled with at least 90% confidence also had alignment coverage of at least 10%, representing 80% of the T. pallidum proteome, (Fig. 2b). All 780 T. pallidum proteins modeled with at least 90% confidence and 10% alignment coverage together with all corresponding model details are listed in Additional file 1: Table S1 (part 2). Furthermore, 681/782 (87%) high confidence-scoring models/templates were based on alignments that covered at least 50% of the T. pallidum protein, representing 70% (681/978) of all T. pallidum predicted protein-coding genes (Fig. 2b). Taken together, these results show that Phyre2-based whole proteome analysis is capable of predicting tertiary structure models with high confidence and biologically relevant alignment coverage for the majority of T. pallidum proteins.
Comparison of T. pallidum primary structure- and tertiary structure-based proteome annotations
Most modern genome annotation pipelines are multi-level processes, however they still rely on inferring protein function from primary structure homology. Six hundred and five of the 780 T. pallidum proteins modeled with high confidence and alignment coverage in the current study were previously annotated with specific functions in the published T. pallidum genome. To determine the level of annotation agreement between the two methods, we compared the 605 Phyre2-predicted protein functions (derived from their model template functions) with the corresponding published protein annotations  (Fig. 1c). When comparing only the top-ranked Phyre2 protein template match for predicting function, 458/605 (76%) were assigned the same function as the published genomic annotations (Fig. 3a). Genome annotations were also compared to all confident (≥90% confidence and ≥ 10% coverage alignment) top 20-ranking templates used to model each protein and a functional match was assigned when the genome annotation matched at least one protein template function. Using this comparison, 525/605 (87%) were assigned the same functions with respect to the published genome (Fig. 3b). Seventeen proteins were also confidently modeled on protein templates whose functions differed from the corresponding genomic annotations (Fig. 3b). This was most likely due to the fact that solved structures of these 17 proteins have not yet been deposited in the PDB. All proteins with the same, related, or different functions were categorized according to their PDB functional classification (Fig. 3c [genome annotation versus top-ranking template only comparison] and Fig. 3d [genome annotation versus all confident top 20-ranking templates for comparison]) and are listed in Additional file 4: Table S4 (top matching template only comparison) and Additional file 5: Table S5 (all confident matching templates used for comparison).
Importantly, when we also compared our molecular modeling-based protein function predictions with T. pallidum proteins that have been characterized experimentally, we observed a high degree of correlation at both the structural and functional levels. For this analysis, we focused on 11 functionally-uncharacterized proteins that were successfully assigned specific functions (TPANIC_0163 [TroA] , TPANIC_0298 [RfuA] , TPANIC_0319 [PnrA] , TPANIC_0435 [Tp17] , TPANIC_0574 [Tp47] , TPANIC_0655 [TpPotD] , TPANIC_0796 [Ftp] , TPANIC_0821 [Tp32] , TPANIC_0956 [TatT] , TPANIC_0957 [TatP(T)] , TPANIC_0971 [Tp34] ) using a structure-to-function approach comprised of three major stages; (1) X-ray crystallography that determined the high resolution structures of the 11 functionally-uncharacterized treponemal proteins, (2) protein structure analyses for hypothesizing potential protein functions, and (3) biochemical characterization of the proteins to confirm or reject the hypotheses. When comparing only the top-ranked Phyre2 protein model and template match for predicting function, ten of the 11 T. pallidum proteins were modeled on the functionally-characterized proteins listed above (Additional file 1: Table S1 [part 1]). The exception was TPANIC_0435 (Tp17) which was modeled with highest confidence on a putative lipoprotein from the proteobacterium, Shewanella oneidensis (100% confidence, 71% alignment coverage). However, this protein was also modeled with a very similar high level of confidence against the Tp17 structural template (99.7% confidence, 76% alignment coverage) (Additional file 1: Table S1 [part 1]). A contributing factor that may partially account for this discrepancy is the fact that the protein structure of the top-ranking model from S. oneidensis was solved at greater resolution than the T. pallidum Tp17 protein (1.42 Å versus 2.4 Å). It should also be noted that Phyre2 indicated that the Tp17 model template exhibited 100% sequence identity to the query T. pallidum protein TPANIC_0435. Although this study focused on using tertiary structure modeling to predict protein function in the absence of high amino acid sequence homology, this finding would need to be taken into consideration when deciding which model is likely more accurate. When comparing all remaining confident (≥90% confidence and ≥ 10% alignment coverage) top 20-ranking templates used to model each of the 11 proteins (ranks 2–20), 11/11 were modeled against protein templates with the same, or very closely related, functions as the 11 functionally-characterized proteins listed above (Additional file 1: Table S1 [part 1]). Together, these findings suggest tertiary structure modeling may serve as a complementary tool to genome sequencing/annotation pipelines for assigning potential protein functions on a proteome-wide scale.
Predicted annotation of T. pallidum proteins of unknown function
In the current study, 175 of the 780 T. pallidum proteins modeled with high confidence/alignment coverage were annotated in the published proteome as “hypothetical” proteins (142/175), putative membrane proteins (27/175), or putative outer membrane proteins (6/175)  (Additional file 6: Table S6). DNA microarray-based analysis of the T. pallidum transcriptome following experimental rabbit infection  showed that almost all of the genes encoding these uncharacterized proteins (173/175) are expressed at the transcription level (Additional file 6: Table S6). In fact, their mean transcript expression level (cDNA/DNA ratio = 1.34) was found to be higher than the mean transcript level (cDNA/DNA ratio = 1.0) of all T. pallidum genes in the array (Additional file 6: Table S6). Furthermore, two mass spectrometry-based proteomics studies also detected peptides from 107 of the 175 uncharacterized proteins during rabbit infection [47, 48] (Additional file 6: Table S6).
Although the potential functions of some of these 175 proteins of unknown function have been investigated within laboratory settings [17, 49,50,51,52,53] (Additional file 6: Table S6), we decided to focus on all of these proteins in order to gain further insight into the unusual biology of T. pallidum (Fig. 1d). When analyzed using only the highest ranking confident tertiary structure model protein template as an indication of protein function, 147/175 (84%) were assigned potential functions (≥90% confidence and ≥ 10% alignment coverage) (Fig. 4a and Additional file 6: Table S6). The 28 uncharacterized proteins that were modeled against structures classified by PDB as “unknown function” are listed in Additional file 7: Table S7. Of the 175 T. pallidum proteins of unknown function, 38 (21.7%) were modeled against hydrolases using PDB functional classifications (Fig. 4a). As expected from this broad classification system, this group included proteins with a diverse range of predicted functions (Additional file 6: Table S6). Predicted hydrolases modeled with very high confidence and alignment coverages included nucleases (TPANIC_0803 [Single-stranded DNA specific exonuclease RecJ] and TPANIC_0489 [Ribonuclease Z]), an esterase (TPANIC_0935; esterase E40), and several carbohydrate-degrading enzymes (e.g. TPANIC_0358; alpha-amylase). Transferases represented the second most abundant predicted functional class for uncharacterized T. pallidum proteins (22/175 [13%]) which also covered a diverse array of functions. Predicted transferases modeled with very high confidence and alignment coverages included a protein kinase (TPANIC_0307; serine/threonine kinase PrkC), a methyltransferase (TPANIC_0032; 16 s rRNA methyltransferase RsmE), and several glycosyltransferases (e.g. TPANIC_0286; Polypeptide GalNAc transferase-2) (Additional file 6: Table S6). Other functionally unannotated T. pallidum proteins of note that were modeled with very high confidence and coverage alignments include TPANIC_0118 (modeled against colicin Ia), TPANIC_0333 (modeled against outer membrane lipoprotein carrier protein, LolA), and five proteins (TPANIC_0548, 0856, 0858, 0859, and 0865) which were modeled on the outer membrane toluene transport protein (TbuX) from the Gram-negative opportunistic pathogen, Ralstonia pickettii (Additional file 6: Table S6).
When T. pallidum proteins of unknown function were analyzed using all high confidence tertiary structure model protein templates as an indication of function (≥90% confidence, ≥10% alignment coverage and at least one of the top 20-ranking predicted tertiary structures modeled against a protein template of known function), 167/175 (95%) uncharacterized proteins were assigned potential functions (Fig. 4b; compared to 147/175 uncharacterized proteins assigned potential functions based on the top ranking template, see above). The additional 20 hypothetical proteins with predicted functions are listed in Additional file 8: Table S8. These included a protein predicted to adopt a tertiary structure fold highly similar to an outer membrane deacetylase involved in facilitating the export of a biofilm adhesin polysaccharide (TPANIC_0083; Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase, PgaB), an antibiotic resistance protein (Tpanic-0405; McbG-like protein), a cell division activator (TPANIC_0846; cell division protein, ZapA), a transcriptional activator (TPANIC_0875; transcriptional regulator, PspF), a resolvase (TPANIC_0913; Holliday junction resolvase, Hjc), and a transcription termination/anti-termination protein (TPANIC_1032; N-utilization substance G, NusG). The eight additional proteins that were modeled against structures classified by PDB as “unknown function” when all confident tertiary structure model protein template functions were taken into account are listed in Additional file 9: Table S9.
Interestingly, three T. pallidum proteins annotated as “hypothetical”  were predicted to adopt structural folds most similar to protein templates that are functionally classified by the PDB as “Blood Clotting” proteins (TPANIC_0246 and TPANIC_0750; similar to von Willebrand Factor Type A domain [vWFA]) and “Hormone/Growth factor” (TPANIC_0484; similar to Anosmin-1) (Fig. 4a and Additional file 6: Table S6). Several additional T. pallidum proteins of unknown function were also modeled with high confidence against other human / eukaryotic proteins, including tenascin-X, fibrinogen, and ankyrin proteins (Additional files 1, 6, 8: Tables S1, S6, S8). These findings suggest that, similar to other pathogens, molecular mimicry may contribute to T. pallidum pathogenesis.
Identification of potential virulence factors
In contrast to other invasive pathogenic bacteria, genome sequencing and annotation projects have identified very few T. pallidum virulence factors. In light of the fact that T. pallidum is one of the most invasive pathogens known, we sought to use our proteome-wide tertiary structure modeling to (1) help corroborate the annotation of proteins identified as potential virulence factors from recent whole genome sequencing  and comparative genomics [54, 55], (2) identify putative novel virulence functions for proteins previously annotated with non-virulence related functions, and (3) identify hypothetical proteins potentially involved in T. pallidum pathogenesis. Therefore, we analyzed the functions of the template proteins that were used to generate the 780 high-confidence/alignment coverage T. pallidum tertiary structure models, and searched for protein templates that correspond to each of the 3 categories described above.
Modeling of proteins previously annotated as putative virulence factors
As shown in Table 1 and Additional file 10: Table S10, 4/5 proteins previously annotated as putative hemolysins  were predicted to be most structurally similar to an E. coli magnesium/cobalt efflux protein (CorC). However, the same four proteins were also predicted to be structurally-similar (5th ranking template model) to a hemolysin-like protein from the non-pathogenic Gram-positive acidophilic facultative anaerobe, Oenococcus oeni. The remaining putative hemolysin (hemolysin III, TPANIC_1037) only exhibited confident structural homology to human adiponectin receptor protein 1 (100% confidence, 96.6% alignment coverage). These findings suggest that the five proteins are not hemolysins.
Eight T. pallidum proteins were also previously annotated as type 3 (virulence-related) secretory pathway proteins . Our tertiary-based modeling approach generated high confidence models for five of these proteins. Two of the five proteins (TPANIC_0399 and TPANIC_0715) were predicted to adopt structural folds most similar to T3SS proteins (Table 1 and Additional file 10: Table S10). However, the other three proteins were predicted to adopt structural folds most similar to a V-type proton ATPase protein or flagellar proteins. TPANIC_0715, but not TPANIC_0399, was also modeled with lower confidence against a flagellar biosynthesis protein (Additional file 10: Table S10).
A bioinformatics approach which compared the amino acid sequences of all proteins from T. pallidum ssp. pallidum and the closely related (99.8% genomic sequence identity) treponeme T. pallidum ssp. pertenue (causative agent of yaws) enabled the identification of 31 putative T. pallidum ssp. pallidum virulence factors [54, 55]. Twelve of these proteins belong to the evolutionarily distinct Treponema pallidum repeat (Tpr) protein family  and were not modeled with high confidence in the current study. Thirteen of the remaining 19 putative virulence factors were modeled with high confidence (Additional file 1: Table S1 and Additional file 11: Table S11). With the exception of the BamA ortholog (TPANIC_0326), TPANIC_0399 and TPANIC_0715 (see below), the potential roles of these proteins in T. pallidum pathogenesis remain unknown.
Identification of potential novel virulence-related functions for annotated proteins
In the present study, three predicted T. pallidum protein-coding genes previously annotated with functions not directly linked to virulence were identified with high confidence by tertiary structure modeling as proteins that may play roles in T. pallidum pathogenesis; TPANIC_0262 (cyclic-nucleotide binding protein), TPANIC_0862 (peptidylprolyl isomerase), and TPANIC_1033 (patatin family phospholipase) (Table 2 and Additional file 12: Table S12).
Our analyses predicted TPANIC_0262 to be most structurally similar to the Listeria monocytogenes virulence factor transcriptional regulator, PrfA (100% confidence and 93.3% alignment coverage). This is consistent with a study that demonstrated TPANIC_0262 is a transcriptional modulator of members of the T. pallidum repeat (tpr) gene family, a group of virulence factor candidates . Tertiary structure modeling identified TPANIC_0862 as a structural homolog of the essential virulence factor associated with macrophage infectivity in Legionella pneumophila, Mip [57, 58] (Macrophage infectivity potentiator; 100% confidence and 73.1% alignment coverage). The top two structural matches for TPANIC_1033 were cytotoxins, namely, the L. pneumophila phospholipase effector VipD  and the Pseudomonas aeruginosa T3SS effector ExoU  (both modeled at 100% confidence and 90.4% / 99.7% alignment coverages, respectively).
Identification of hypothetical proteins with potential roles in T. pallidum virulence
Twenty-one of the 175 T. pallidum proteins of unknown function that were modeled with high confidence in the current study were predicted to exhibit high structural similarity with proteins known to be required for, or involved in, the virulence of other pathogens. These 21 T. pallidum proteins and their virulence model templates are summarized in Table 3 and Phyre2 modeling/virulence template details are listed in Additional file 13: Table S13.
Seven of the 21 T. pallidum hypothetical proteins were predicted to be structural orthologs of virulence factors that are involved in mediating host adhesion and invasion (TPANIC_0020, TPANIC_0126, TPANIC_0225, TPANIC_0246, TPANIC_0421, TPANIC_0733, and TPANIC_0911). Four proteins exhibited high structural homology to orthologs involved in type 1 secretion and drug efflux (TPANIC_0966–0969). Phyre2 also predicted high structural similarity of three hypothetical proteins to immune modulators (TPANIC_0126, TPANIC_0733, and TPANIC_0789) and cytotoxins (TPANIC_0126, TPANIC_0544, and TPANIC_0579). Two proteins were modeled on sialidases (TPANIC_0134 and TPANIC_0854), and one on a protein that functions in host colonization and persistence (TPANIC_0594). Five of the 21 proteins were modeled against proteins involved in the folding, assembly, translocation, and insertion of proteins in the outer membrane (TPANIC_0598, TPANIC_0625, TPANIC_0783, TPANIC_0789, and TPANIC_0928).
DNA microarray-based analysis of the T. pallidum transcriptome following experimental rabbit infection showed that the genes encoding 11 of the 21 uncharacterized proteins identified as virulence factor candidates in the current study were all expressed at higher levels (cDNA/DNA ratio > 1.0) than the mean transcript level (cDNA/DNA ratio = 1.0) of all T. pallidum genes in the array  (Table 3). In fact, eight of these potential virulence factor genes were expressed at approximately two- to four-fold higher levels than the mean T. pallidum transcript level whereas four of the 21 potential virulence factor genes were identified as being weakly expressed during infection (cDNA/DNA ratio ≤ 0.36)  (Table 3). Furthermore, mass spectrometry-based proteomics studies have also detected peptides from 14 of the 21 uncharacterized proteins identified as putative virulence factors, demonstrating that at least two thirds of these proteins are expressed during rabbit infection [47, 48] (Table 3). Interestingly, 7/21 (33%) hypothetical proteins identified with potential roles in T. pallidum virulence were also previously shown to be among the set of orthologous proteins from the closely-related treponemes, T. pallidum ssp. pertenue and T. paraluiscuniculi, that contain major sequence changes (MSC) (Table 3) [55, 61]. In contrast, only 89 proteins from the whole T. pallidum proteome (9%) were found to contain major sequence differences when compared to the closely-related treponemal orthologs (p = 0.002; Fisher’s exact test) .
Using a computational modeling-based approach, we generated high-confidence tertiary structure models and inferred functional annotations for 80% of the T. pallidum proteome. Out of the 196 proteins modeled with low confidence (20% of the T. pallidum proteome), 150 proteins were functionally-unannotated in the published T. pallidum proteome, . The high number of functionally-unannotated proteins modeled with low confidence further highlights the phylogenetic divergence of T. pallidum. Members of this structurally-divergent and functionally-uncharacterized protein subset may contribute to the unusual structural and virulence properties of T. pallidum. In contrast, almost a quarter of the proteins that were modeled with low-confidence had previously been assigned specific functions in the published T. pallidum proteome . The physicochemical properties of this group of proteins varied widely. In addition, the average values corresponding to some of the physicochemical properties were similar to those previously reported for all T. pallidum proteins , including average molecular mass and isoelectric point. Therefore, unusual physicochemical characteristics cannot be correlated with the failure to generate high-confidence models for this group of proteins. Several of the proteins in this group belong to the phylogenetically distinct Tpr family of proteins , which may partially explain why almost a quarter of these proteins were not modeled with high confidence. Subcellular localization analyses predicted half of the proteins to be localized to the inner membrane. However, many high-confidence tertiary structure models of known and predicted inner membrane proteins were generated in the current study, indicating this predicted subcellular localization bias is also unlikely to explain the inability to generate high-confidence models for this group of 46 proteins that have been previously annotated using genome sequencing/annotation pipelines. The most likely explanation why these 46 proteins were annotated based on primary structure but not by tertiary structure modeling is that the proteins share some degree of amino acid sequence homology with other annotated proteins, however high resolution structures of these proteins have not been deposited in the PDB.
Importantly, our tertiary structure modeling pipeline was able to predict the same function as primary structure-based annotations from genome sequencing/annotation pipelines for almost 90% of compared proteins. In addition, we observed a high degree of correlation at both the structural and functional levels when we compared our molecular modeling-based protein function predictions with the functions reported for 11 T. pallidum proteins that were assigned specific functions using structure-to-function experimental approaches. Together, these results (1) suggest our structural bioinformatics pipeline is a promising approach for assigning tentative protein functions based on tertiary structural modeling on a proteome-wide level, and (2) strengthen the published primary structure-based annotation of more than half of all T. pallidum proteins. This latter finding suggests this approach could be used as a complementary bioinformatics tool to corroborate primary structure-based methodologies in genome annotation pipelines. Furthermore, confident tertiary structure modeling-based annotation of proteins that are found to have different functional predictions from primary structure homology-based annotations could help identify potential misannotated proteins in genome sequencing pipelines.
Treponema pallidum is a phylogenetically distinct bacterium, highlighted by the fact that one third (327/978) of predicted protein-coding genes have no known orthologs and/or assigned function . Furthermore, at 1.14 Mb, T. pallidum has the smallest spirochetal genome and one of the smallest bacterial genomes sequenced to date . Obligate pathogenic bacteria, such as T. pallidum, often undergo genomic reduction characterized by the loss of non-essential genes as an evolutionary adaptation mechanism for the rich and complex host environment that is encountered during infection [63, 64]. Thus, it is likely that the T. pallidum proteins of unknown function play important roles in the biology of this treponeme, and may also contribute to its unique structural and virulence properties.
In the current study, 175 of the 327 T. pallidum proteins with no known orthologs and/or assigned function  were modeled with high confidence/alignment coverage. Of these 175 proteins, 167 (95%) were assigned predicted biological roles based on the function of the corresponding model templates. Importantly, previous expression-profiling –omics studies [45, 47, 48] have also demonstrated that (1) 99% of the 175 genes predicted to encode T. pallidum proteins of unknown function, including the majority that are annotated as “hypothetical proteins”, represent functional genes, and (2) at least 61% of these functionally-uncharacterized T. pallidum proteins are expressed during infection.
Most of the 175 T. pallidum proteins of unknown function were modeled against hydrolases and transferases using PDB functional classifications. Interestingly, three “hypothetical” T. pallidum proteins  were also predicted to adopt structural folds similar to “Blood Clotting” proteins (TPANIC_0246 and TPANIC_0750) and a “Hormone/Growth factor” (TPANIC_0484). Specifically, Phyre2 predicted structural similarity of TPANIC_0246 and TPANIC_0750 to von Willebrand Factor Type A domain (vWFA), the prototype domain of the vWF domain-containing protein superfamily . The TPANIC_0750 result is in agreement with previous molecular modeling that also predicted similarity to vWFA . The tertiary structure of TPANIC_0484 was predicted to be most similar to Human Anosmin-1, a fibronectin type 3 domain-containing extracellular matrix protein involved in nerve cell migration, axon outgrowth, and nerve cell adhesion [66, 67]. The finding that a subset of T. pallidum proteins of unknown function is predicted to adopt structural folds that closely resemble host proteins suggests that T. pallidum may use molecular mimicry as a pathogenic strategy for the subversion and exploitation of normal host cellular functions during infection.
It has been demonstrated, using high-resolution microscopy techniques, that T. pallidum contains few outer membrane proteins [9, 10]. Interestingly, our analyses identified five functionally-unannotated T. pallidum proteins (TPANIC_0548, 0856, 0858, 0859, and 0865) which were modeled on the toluene transport protein (TbuX) from the Gram-negative opportunistic pathogen, Ralstonia pickettii. This 14-stranded beta barrel-containing outer membrane protein belongs to the FadL fatty acid transporter family [68, 69] and functions in the uptake and degradation of aromatic hydrocarbons, including toluene [70, 71]. Given that T. pallidum is unlikely to encounter aromatic hydrocarbons during infection and that aromatic hydrocarbon degradation pathways have not been identified in the T. pallidum genome [7, 8], it is likely that the substrate specificities of the five potential T. pallidum outer membrane transport proteins differ from TbuX. In agreement with a previous molecular modeling study , these five T. pallidum proteins were also modeled on FadL from E. coli and P. aeruginosa with similar high confidence and alignment coverage scores as those observed for the top ranking model template, TbuX. Treponema pallidum has very limited biosynthetic capabilities, including its inability to synthesize fatty acids de novo, and must therefore rely on the host for nutrients . Thus, these findings suggest that the five potential T. pallidum transport proteins are more likely to be involved in the transport of hydrophobic molecules such as fatty acids across the outer membrane during infection.
Genome sequencing and annotation projects have identified very few T. pallidum proteins that are potential orthologs of known virulence factors from other pathogenic bacteria [7, 8]. This finding is surprising as T. pallidum is a strict pathogen capable of infecting every tissue and organ, and is one of the most invasive pathogens known. Five potential hemolysins were identified in T. pallidum genome sequencing pipelines [7, 8], however tertiary structure modeling suggests that these proteins are unlikely to function as toxins which is consistent with previous results that failed to show hemolytic activity for recombinant forms of these proteins [62, 73]. A further eight T. pallidum proteins have been annotated in the Nichols strain as T3SS proteins . Five of these proteins were modeled with high confidence/alignment coverage in the present study, two of which were also predicted to adopt structural folds similar to T3SS proteins. Two evolutionarily-related, but functionally-distinct, forms of T3SS are often found in Gram-negative bacteria that are responsible for mediating translocation of proteins across the inner and outer membranes: (1) the flagellar T3SS that mediates transport and export of proteins that comprise the flagellar filament structure used for motility and (2) the non-flagellar T3SS (also known as the injectisome) that secretes effectors into host cells [74, 75]. These two T3SSs are comprised of several orthologous proteins and also share several ultrastructural similarities . Treponema pallidum possesses flagellar filaments that are located entirely within the periplasmic space between the inner and outer membranes [9, 10]. However, to date no evidence of an injectisome or needle-like T3SS structure has been detected on the surface of T. pallidum, including through the use of high-resolution cryo electron tomography [9, 10]. In addition, several protein structures from both the flagellar and non-flagellar/injectisome T3SSs remain to be solved and deposited in the PDB. Therefore, it is possible that proteins modeled by Phyre2 as injectisome-like T3SS-associated proteins actually belong to the flagellar T3SS subtype. Interestingly, examples of T3SSs have been reported that do not conform fully to either subtype. For example, it has been reported that flagellar T3SS of some pathogenic bacteria have also evolved the ability to secrete virulence factors [77,78,79]. Thus, another possibility is that the flagellar T3SS of T. pallidum has also evolved the ability to transport, and possibly export, non-flagellar filament proteins. This hybrid export system would be consistent with the highly invasive nature of T. pallidum and its obligate pathogenic lifestyle and resulting reduced genome. If this system can indeed function as a T3SS, the mechanism of effector export and the export machinery would be predicted to differ greatly from the injectisome needle complexes found in conventional Gram-negative pathogens, such as Salmonella typhimurium, owing to the phylogenetic divergence of this pathogen and its unusual subcellular flagella location.
Twenty-one T. pallidum proteins of unknown function were predicted to exhibit structural similarity with proteins experimentally confirmed to be required for virulence in other pathogens. These proteins were modeled on virulence factors with roles in host adhesion and invasion, type 1 secretion and drug efflux, immune modulation, cytotoxicity, sialidase activity, host colonization and persistence, and five proteins that function in the folding, assembly, translocation, and insertion of proteins in the outer membrane. Although the protein templates used to model these latter five proteins are normally located within the periplasm of other bacteria and do not mediate direct interactions with the host, they are important for outer membrane protein localization and surface exposure, which is an essential mechanism for facilitating host-pathogen interactions during infection. Importantly, some of our tertiary structure modeling findings correlated with an alternative molecular modeling approach used by Radolf and Kumar . Specifically, three potential virulence factors identified in the current work (Tp0733, Tp0966 and Tp0967) were modeled in both studies against protein templates known to adopt beta barrel folds, that localize to the outer membrane, and that function in complement resistance and drug efflux. In the absence of high sequence identity, these findings have tentatively identified several uncharacterized proteins that, based on high-confidence structural modeling predictions, may represent important mediators of T. pallidum virulence.
The clinical manifestations of infections with T. pallidum subspecies pallidum and the less-invasive subspecies pertenue are readily distinguished. However, a comparative genomics study demonstrated identical gene synteny and 99.8% genetic identity between the genomes . Similarly, rabbit venereal spirochetosis-causing Treponema paraluiscuniculi shares greater than 99% sequence identity in conserved regions and identical gene synteny with T. pallidum . However, T. paraluiscuniculi is non-infectious in humans and rabbit infection is also characterized by different and less-invasive disease symptoms. Orthologous proteins from these closely-related pathogens that contain at least one MSC may therefore represent potential treponemal virulence factors that could account for differences in host specificity and clinical manifestations [55, 61]. Out of the 21 T. pallidum putative virulence factors identified in the current study, seven (33%) were previously shown to contain at least one MSC when compared with the corresponding orthologous proteins in the two closely-related treponemes described above [55, 61]. However, a total of only 89 MSC-containing proteins were identified in the whole T. pallidum proteome (9%) . The high proportion of hypothetical proteins with potential virulence roles that also harbour major primary structure differences compared to their orthologs in less-invasive subspecies and T. paraluiscuniculi is consistent with our tertiary structure modeling findings that suggest roles for this subset of proteins in the pathogenesis of T. pallidum subspecies pallidum.
Here we employed a high throughput structural bioinformatics approach for the prediction of protein function from tertiary structure modeling on a proteome-wide scale, often in the absence of significant amino acid sequence homology. However, as with other methods for predicting protein function, there are limitations associated with this approach. First, high confidence tertiary structure modeling relies entirely on the deposition of high-resolution protein structures in the PDB, that through structural similarities serve as protein templates for predicting the three dimensional fold of the query protein. If these structural templates have not been solved and/or deposited in the PDB, then tertiary structure modeling and function prediction is not possible. Second, assignment of predicted protein functions is complicated when the query protein (or a specific region of the query protein) is modeled against several functionally diverse protein templates which all have similar high confidence and alignment coverage scores. In this scenario, the amino acid identities of the query sequence and protein templates may be taken into account to identify any template(s) with significantly higher amino acid homology as increasing identity often correlates with an increased likelihood of related function. Third, predicting functions for protein structures modeled with high confidence but low alignment coverage increases the risk of misannotation of protein function. For example, the function of the template used to model a single domain or isolated region that corresponds to only 10–20% of amino acids in the query protein sequence may not be representative of the overall/multiple functions of the full-length query protein. However, in the present study only 5% of all high confidence (≥90%) models were based on alignment coverages of 20% or less. Conversely, almost three quarters of all T. pallidum proteins were confidently modeled with at least 50% coverage and over half of all T. pallidum proteins were confidently modeled with at least 80% alignment coverage. Thus, the risk of functional annotation errors for T. pallidum proteins due to low sequence coverage in the current study is very low. However, it is important to note that tertiary structure model alignment coverage refers only to the percentage of amino acids in the query protein sequence that align with the template protein, but does not provide any information regarding the size or percentage of amino acids of the model template protein that was used to generate the alignment. Therefore, caution must be taken in assigning function to a query protein based on a tertiary structure model template that contains additional functionally-characterized domains that may have been used to annotate the function of the template protein during the genome sequencing and annotation pipeline. Finally, it should be noted that this molecular modeling-based annotation approach has assigned protein functions in the absence of experimental validation, and that these putative functions can only be truly validated experimentally.
In the present study, we used the tertiary structure modeling server, Phyre2, to investigate the potential function of all 978 predicted proteins from the causative agent of syphilis, Treponema pallidum subspecies pallidum (Nichols strain). This structural modeling-based approach provided insight into the putative functions of approximately 80% of T. pallidum proteins. Furthermore, functional predictions inferred from proteome-wide structural modeling corroborated the amino acid-based annotation of over half of all T. pallidum proteins, suggesting that this methodology is useful for increasing confidence in the annotated predicted protein functions derived from genome sequencing projects. Importantly, our tertiary structure modeling approach was also able to predict structural models based on functionally-annotated templates for over half of all uncharacterized T. pallidum proteins, thereby providing novel insight into this enigmatic group of proteins that may have potential roles in the unusual ultrastructure and unique pathogenesis of T. pallidum. This suggests our approach has the potential for enhancing understanding of protein function across all taxonomic groups and may be particularly applicable for the functional annotation of phylogenetically distinct organisms. This is the first report of a proteome-wide structural modeling approach that has provided further insight into the potential functions of the majority of T. pallidum proteins. In light of the dynamic nature of the protein databank and continued technological advances in next-generation sequencing that facilitate the improved sequencing accuracy and annotation of whole genomes, regular re-analyses of whole proteomes using molecular modeling will continue to improve upon our understanding of structure-function relationships and is likely to provide further insight into the molecular mechanisms underlying T. pallidum pathogenesis.
Multiple sequence alignment
Major sequence changes
Protein data bank
Type 3 secretion system
- Tp :
Treponema pallidum Nichols strain (nomenclature used to annotate proteins)
World Health Organization. Global incidence and prevalence of selected curable sexually transmitted infections. 2008. http://www.who.int/reproductivehealth/publications/rtis/stisestimates/en. Accessed 2 May 2018.
Newman L, Kamb M, Hawkes S, Gomez G, Say L, Seuc A, Broutet N. Global estimates of syphilis in pregnancy and associated adverse outcomes: analysis of multinational antenatal surveillance data. PLoS Med. 2013;10(2):e1001396.
Nusbaum MR, Wallace RR, Slatt LM, Kondrad EC. Sexually transmitted infections and increased risk of co-infection with human immunodeficiency virus. J Am Osteopath Assoc. 2004;104(12):527–35.
Lukehart SA, Hook EW, Baker-Zander SA, Collier AC, Critchlow CW, Handsfield HH. Invasion of the central nervous system by Treponema pallidum: implications for diagnosis and treatment. Ann Intern Med. 1988;109(11):855–62.
Cumberland MC, Turner TB. Rate of multiplication of Treponema pallidum in normal and immune rabbits. Amer J Syph. 1949;33:201–12.
Raiziss GW, Severac M. Rapidity with which Spirochaeta pallida invades the bloodstream. Arch Dermatol Syphilol. 1937;35:1101–9.
Fraser CM, Norris SJ, Weinstock GM, White O, Sutton GG, Dodson R, Gwinn M, Hickey EK, Clayton R, Ketchum KA, et al. Complete genome sequence of Treponema pallidum, the syphilis spirochete. Science. 1998;281(5375):375–88.
Pӗtrošová H, Pospíšilová P, Strouhal M, Čejková D, Zobaníková M, Mikalová L, Sodergren E, Weinstock GM, Šmajs D. Resequencing of Treponema pallidum ssp. pallidum strains Nichols and SS14: correction of sequencing errors resulted in increased separation of syphilis treponeme subclusters. PLoS One. 2013;8(9):e74319.
Izard J, Renken C, Hsieh CE, Desrosiers DC, Dunham-Ems S, La VC, Gebhardt LL, Limberger RJ, Cox DL, Marko M, et al. Cryo-electron tomography elucidates the molecular architecture of Treponema pallidum, the syphilis spirochete. J Bacteriol. 2009;191(24):7566–80.
Liu J, Howell JK, Bradley SD, Zheng Y, Zhou ZH, Norris SJ. Cellular architecture of Treponema pallidum: novel flagellum, periplasmic cone, and cell envelope as revealed by cryo electron tomography. J Mol Biol. 2010;403(4):546–61.
Illergard K, Ardell DH, Elofsson A. Structure is three to ten times more conserved than sequence - a study of structural response in protein cores. Proteins. 2009;77(3):499–508.
Berman HM, Westbrook J, Feng Z, Gilliland G, Bhat TN, Weissig H, Shindyalov IN, Bourne PE. The Protein Data Bank. Nucleic Acids Res. 2000;28(1):235–42.
The Research Collaboratory for Structural Bioinformatics Protein Data Bank. http://www.rcsb.org. Accessed 2 May 2018.
Parker ML, Houston S, Petrosova H, Lithgow KV, Hof R, Wetherell C, Kao WC, Lin YP, Moriarty TJ, Ebady R, et al. The structure of Treponema pallidum Tp0751 (Pallilysin) reveals a non-canonical lipocalin fold that mediates adhesion to extracellular matrix components and interactions with host cells. PLoS Pathog. 2016;12(9):e1005919.
Parker ML, Houston S, Wetherell C, Cameron CE, Boulanger MJ. The structure of Treponema pallidum Tp0624 reveals a modular assembly of divergently functionalized and previously uncharacterized domains. PLoS One. 2016;11(11):e0166274.
Deka RK, Machius M, Norgard MV, Tomchick DR. Crystal structure of the 47-kDa lipoprotein of Treponema pallidum reveals a novel penicillin-binding protein. J Biol Chem. 2002;277(44):41857–64.
Deka RK, Brautigam CA, Goldberg M, Schuck P, Tomchick DR, Norgard MV. Structural, bioinformatic, and in vivo analyses of two Treponema pallidum lipoproteins reveal a unique TRAP transporter. J Mol Biol. 2012;416(5):678–96.
Deka RK, Neil L, Hagman KE, Machius M, Tomchick DR, Brautigam CA, Norgard MV. Structural evidence that the 32-kilodalton lipoprotein (Tp32) of Treponema pallidum is an L-methionine-binding protein. J Biol Chem. 2004;279(53):55644–50.
Santos-Silva T, Trincao J, Carvalho AL, Bonifacio C, Auchere F, Raleiras P, Moura I, Moura JJ, Romao MJ. The first crystal structure of class III superoxide reductase from Treponema pallidum. J Biol Inorg Chem. 2006;11(5):548–58.
Thumiger A, Polenghi A, Papinutto E, Battistutta R, Montecucco C, Zanotti G. Crystal structure of antigen TpF1 from Treponema pallidum. Proteins. 2006;62(3):827–30.
Deka RK, Brautigam CA, Yang XF, Blevins JS, Machius M, Tomchick DR, Norgard MV. The PnrA (Tp0319; TmpC) lipoprotein represents a new family of bacterial purine nucleoside receptor encoded within an ATP-binding cassette (ABC)-like operon in Treponema pallidum. J Biol Chem. 2006;281(12):8072–81.
Deka RK, Brautigam CA, Tomson FL, Lumpkins SB, Tomchick DR, Machius M, Norgard MV. Crystal structure of the Tp34 (TP0971) lipoprotein of Treponema pallidum: implications of its metal-bound state and affinity for human lactoferrin. J Biol Chem. 2007;282(8):5944–58.
Machius M, Brautigam CA, Tomchick DR, Ward P, Otwinowski Z, Blevins JS, Deka RK, Norgard MV. Structural and biochemical basis for polyamine binding to the Tp0655 lipoprotein of Treponema pallidum: putative role for Tp0655 (TpPotD) as a polyamine receptor. J Mol Biol. 2007;373(3):681–94.
Brautigam CA, Deka RK, Liu WZ, Norgard MV. Insights into the potential function and membrane organization of the TP0435 (Tp17) lipoprotein from Treponema pallidum derived from structural and biophysical analyses. Protein Sci. 2015;24(1):11–9.
Brautigam CA, Deka RK, Liu WZ, Tomchick DR, Norgard MV. Functional clues from the crystal structure of an orphan periplasmic ligand-binding protein from Treponema pallidum. Protein Sci. 2017;26(4):847–56.
Lee YH, Deka RK, Norgard MV, Radolf JD, Hasemann CA. Treponema pallidum TroA is a periplasmic zinc-binding protein with a helical backbone. Nat Struct Biol. 1999;6(7):628–33.
Deka RK, Brautigam CA, Liu WZ, Tomchick DR, Norgard MV. The TP0796 lipoprotein of Treponema pallidum is a bimetal-dependent FAD pyrophosphatase with a potential role in flavin homeostasis. J Biol Chem. 2013;288(16):11106–21.
Brautigam CA, Deka RK, Liu WZ, Norgard MV. The Tp0684 (MglB-2) lipoprotein of Treponema pallidum: a glucose-binding protein with divergent topology. PLoS One. 2016;11(8):e0161022.
Brautigam CA, Deka RK, Schuck P, Tomchick DR, Norgard MV. Structural and thermodynamic characterization of the interaction between two periplasmic Treponema pallidum lipoproteins that are components of a TPR-protein-associated TRAP transporter (TPAT). J Mol Biol. 2012;420(1–2):70–86.
Deka RK, Brautigam CA, Biddy BA, Liu WZ, Norgard MV. Evidence for an ABC-type riboflavin transporter system in pathogenic spirochetes. Mbio. 2013;4(1):e00615–12.
Luthra A, Zhu G, Desrosiers DC, Eggers CH, Mulay V, Anand A, McArthur FA, Romano FB, Caimano MJ, Heuck AP, et al. The transition from closed to open conformation of Treponema pallidum outer membrane-associated lipoprotein TP0453 involves membrane sensing and integration by two amphipathic helices. J Biol Chem. 2011;286(48):41656–68.
Kelley LA, Mezulis S, Yates CM, Wass MN, Sternberg MJ. The Phyre2 web portal for protein modeling, prediction and analysis. Nat Protoc. 2015;10(6):845–58.
Moult J, Fidelis K, Kryshtafovych A, Schwede T, Tramontano A. Critical assessment of methods of protein structure prediction (CASP) - round X. Proteins. 2014;82(2):1–6.
Protein Structure Prediction Center. http://predictioncenter.org/index.cgi. Accessed 2 May 2018.
Mao C, Shukla M, Larrouy-Maumus G, Dix FL, Kelley LA, Sternberg MJ, Sobral BW, de Carvalho LP. Functional assignment of Mycobacterium tuberculosis proteome revealed by genome-scale fold-recognition. Tuberculosis. 2013;93(1):40–6.
Lewis TE, Sillitoe I, Andreeva A, Blundell TL, Buchan DW, Chothia C, Cuff A, Dana JM, Filippis I, Gough J, et al. Genome3D: a UK collaborative project to annotate genomic sequences with predicted 3D structures based on SCOP and CATH domains. Nucleic Acids Res. 2013;41(Database issue):D499–507.
Fucile G, Di Biase D, Nahal H, La G, Khodabandeh S, Chen Y, Easley K, Christendat D, Kelley L, Provart NJ. ePlant and the 3D data display initiative: integrative systems biology on the world wide web. PLoS One. 2011;6(1):e15237.
Phyre2: Protein Homology/analogy Recognition Engine V 2.0. http://www.sbg.bio.ic.ac.uk/phyre2. Accessed 2 May 2018.
Remmert M, Biegert A, Hauser A, Soding J. HHblits. Lightning-fast iterative protein sequence searching by HMM-HMM alignment. Nat Methods. 2011;9(2):173–5.
Jones DT. Protein secondary structure prediction based on position-specific scoring matrices. J Mol Biol. 1999;292(2):195–202.
Soding J. Protein homology detection by HMM-HMM comparison. Bioinformatics. 2005;21(7):951–60.
Xie W, Sahinidis NV. Residue-rotamer-reduction algorithm for the protein side-chain conformation problem. Bioinformatics. 2006;22(2):188–94.
Pettersen EF, Goddard TD, Huang CC, Couch GS, Greenblatt DM, Meng EC, Ferrin TE. UCSF chimera - a visualization system for exploratory research and analysis. J Comput Chem. 2004;25(13):1605–12.
UCSF Chimera: an Extensible Molecular Modeling System. http://www.rbvi.ucsf.edu/chimera/. Accessed 2 May 2018.
Šmajs D, McKevitt M, Howell JK, Norris SJ, Cai WW, Palzkill T, Weinstock GM. Transcriptome of Treponema pallidum: gene expression profile during experimental rabbit infection. J Bacteriol. 2005;187(5):1866–74.
LaFond RE, Lukehart SA. Biological basis for syphilis. Clin Microbiol Rev. 2006;19(1):29–49.
Osbak KK, Houston S, Lithgow KV, Meehan CJ, Strouhal M, Šmajs D, Cameron CE, Van Ostade X, Kenyon CR, Van Raemdonck GA. Characterizing the syphilis-causing Treponema pallidum ssp. pallidum proteome using complementary mass spectrometry. PLoS Negl Trop Dis. 2016;10(9):e0004988.
McGill MA, Edmondson DG, Carroll JA, Cook RG, Orkiszewski RS, Norris SJ. Characterization and serologic analysis of the Treponema pallidum proteome. Infect Immun. 2010;78(6):2631–43.
Giacani L, Brandt SL, Ke W, Reid TB, Molini BJ, Iverson-Cabral S, Ciccarese G, Drago F, Lukehart SA, Centurion-Lara A. Transcription of TP0126, Treponema pallidum putative OmpW homolog, is regulated by the length of a homopolymeric guanosine repeat. Infect Immun. 2015;83(6):2275–89.
Cameron CE, Brown EL, Kuroiwa JMY, Schnapp LM, Brouwer NL. Treponema pallidum fibronectin-binding proteins. J Bacteriol. 2004;186:7019–22.
Shevchenko DV, Akins DR, Robinson E, Li M, Popova TG, Cox DL, Radolf JD. Molecular characterization and cellular localization of TpLRR, a processed leucine-rich repeat protein of Treponema pallidum, the syphilis spirochete. J Bacteriol. 1997;179(10):3188–95.
Hazlett KR, Cox DL, Decaffmeyer M, Bennett MP, Desrosiers DC, La Vake CJ, La Vake ME, Bourell KW, Robinson EJ, Brasseur R, et al. Tp0453, a concealed outer membrane protein of Treponema pallidum, enhances membrane permeability. J Bacteriol. 2005;187(18):6499–508.
Houston S, Russell S, Hof R, Roberts AK, Cullen P, Irvine K, Smith DS, Borchers CH, Tonkin ML, Boulanger MJ, et al. The multifunctional role of the pallilysin-associated Treponema pallidum protein, Tp0750, in promoting fibrinolysis and extracellular matrix component degradation. Mol Microbiol. 2014;91(3):618–34.
Pӗtrošová H, Zobaníková M, Čejková D, Mikalová L, Pospíšilová P, Strouhal M, Chen L, Qin X, Muzny DM, Weinstock GM, et al. Whole genome sequence of Treponema pallidum ssp. pallidum, strain Mexico a, suggests recombination between yaws and syphilis strains. PLoS Negl Trop Dis. 2012;6(9):e1832.
Čejková D, Zobaníková M, Chen L, Pospíšilová P, Strouhal M, Qin X, Mikalová L, Norris SJ, Muzny DM, Gibbs RA, et al. Whole genome sequences of three Treponema pallidum ssp. pertenue strains: yaws and syphilis treponemes differ in less than 0.2% of the genome sequence. PLoS Negl Trop Dis. 2012;6(1):e1471.
Giacani L, Godornes C, Puray-Chavez M, Guerra-Giraldez C, Tompa M, Lukehart SA, Centurion-Lara A. TP0262 is a modulator of promoter activity of tpr subfamily II genes of Treponema pallidum ssp. pallidum. Mol Microbiol. 2009;72(5):1087–99.
Engleberg NC, Carter C, Weber DR, Cianciotto NP, Eisenstein BI. DNA sequence of mip, a Legionella pneumophila gene associated with macrophage infectivity. Infect Immun. 1989;57(4):1263–70.
Cianciotto NP, Eisenstein BI, Mody CH, Toews GB, Engleberg NC. A Legionella pneumophila gene encoding a species-specific surface protein potentiates initiation of intracellular infection. Infect Immun. 1989;57(4):1255–62.
Gaspar AH, Machner MP. VipD is a Rab5-activated phospholipase A1 that protects Legionella pneumophila from endosomal fusion. Proc Natl Acad Sci. 2014;111(12):4560–5.
Finck-Barbancon V, Goranson J, Zhu L, Sawa T, Wiener-Kronish JP, Fleiszig SM, Wu C, Mende-Mueller L, Frank DW. ExoU expression by Pseudomonas aeruginosa correlates with acute cytotoxicity and epithelial injury. Mol Microbiol. 1997;25(3):547–57.
Šmajs D, Zobaníková M, Strouhal M, Čejková D, Dugan-Rocha S, Pospíšilová P, Norris SJ, Albert T, Qin X, Hallsworth-Pepin K, et al. Complete genome sequence of Treponema paraluiscuniculi, strain Cuniculi a: the loss of infectivity to humans is associated with genome decay. PLoS One. 2011;6(5):e20415.
Šmajs D, Norris SJ, Weinstock GM. Genetic diversity in Treponema pallidum: implications for pathogenesis, evolution and molecular diagnostics of syphilis and yaws. Infect Genet Evol. 2012;12(2):191–202.
Kelkar YD, Ochman H. Genome reduction promotes increase in protein functional complexity in bacteria. Genetics. 2013;193(1):303–7.
McCutcheon JP, Moran NA. Extreme genome reduction in symbiotic bacteria. Nat Rev Microbiol. 2011;10(1):13–26.
Whittaker CA, Hynes RO. Distribution and evolution of von Willebrand/integrin a domains: widely dispersed domains with roles in cell adhesion and elsewhere. Mol Biol Cell. 2002;13(10):3369–87.
Choy C, Kim SH. Biological actions and interactions of anosmin-1. Front Horm Res. 2010;39:78–93.
de Castro F, Esteban PF, Bribian A, Murcia-Belmonte V, Garcia-Gonzalez D, Clemente D. The adhesion molecule anosmin-1 in neurology: Kallmann syndrome and beyond. Adv Neurobiol. 2014;8:273–92.
Nunn WD, Simons RW. Transport of long-chain fatty acids by Escherichia coli: mapping and characterization of mutants in the fadL gene. Proc Natl Acad Sci. 1978;75(7):3377–81.
Black PN, Said B, Ghosn CR, Beach JV, Nunn WD. Purification and characterization of an outer membrane-bound protein involved in long-chain fatty acid transport in Escherichia coli. J Biol Chem. 1987;262(3):1412–9.
Kahng HY, Byrne AM, Olsen RH, Kukor JJ. Characterization and role of tbuX in utilization of toluene by Ralstonia pickettii PKO1. J Bacteriol. 2000;182(5):1232–42.
Hearn EM, Patel DR, van den Berg B. Outer-membrane transport of aromatic hydrocarbons as a first step in biodegradation. Proc Natl Acad Sci. 2008;105(25):8601–6.
Radolf JD, Kumar S. The Treponema pallidum outer membrane. Curr Top Microbiol Immunol. 2017; https://doi.org/10.1007/82_2017_44.
Weinstock GM, Šmajs D, Hardham J, Norris SJ. From microbial genome sequence to applications. Res Microbiol. 2000;151(2):151–8.
Galan JE, Lara-Tejero M, Marlovits TC, Wagner S. Bacterial type III secretion systems: specialized nanomachines for protein delivery into target cells. Annu Rev Microbiol. 2014;68:415–38.
Aldridge P, Hughes KT. Regulation of flagellar assembly. Curr Opin Microbiol. 2002;5(2):160–5.
Kawamoto A, Morimoto YV, Miyata T, Minamino T, Hughes KT, Kato T, Namba K. Common and distinct structural features of Salmonella injectisome and flagellar basal body. Sci Rep. 2013;3:3369.
Barrero-Tobon AM, Hendrixson DR. Identification and analysis of flagellar coexpressed determinants (Feds) of Campylobacter jejuni involved in colonization. Mol Microbiol. 2012;84(2):352–69.
Young GM, Schmiel DH, Miller VL. A new pathway for the secretion of virulence factors by bacteria: the flagellar export apparatus functions as a protein-secretion system. Proc Natl Acad Sci. 1999;96(11):6456–61.
Guerry P. Campylobacter flagella: not just for motility. Trends Microbiol. 2007;15(10):456–61.
Huynh MH, Carruthers VB. Toxoplasma MIC2 is a major determinant of invasion and virulence. PLoS Pathog. 2006;2(8):e84.
Kappe SH, Buscaglia CA, Nussenzweig V. Plasmodium sporozoite molecular cell biology. Annu Rev Cell Dev Biol. 2004;20:29–59.
Wu XB, Tian LH, Zou HJ, Wang CY, Yu ZQ, Tang CH, Zhao FK, Pan JY. Outer membrane protein OmpW of Escherichia coli is required for resistance to phagocytosis. Res Microbiol. 2013;164(8):848–55.
Krishnan S, Prasadarao NV. Outer membrane protein A and OprF: versatile roles in gram-negative bacterial infections. FEBS J. 2012;279(6):919–31.
McPhee JB, Tamber S, Bains M, Maier E, Gellatly S, Lo A, Benz R, Hancock RE. The major outer membrane protein OprG of Pseudomonas aeruginosa contributes to cytotoxicity and forms an anaerobically regulated, cation-selective channel. FEMS Microbiol Lett. 2009;296(2):241–7.
Azghani AO, Idell S, Bains M, Hancock RE. Pseudomonas aeruginosa outer membrane protein F is an adhesin in bacterial binding to lung epithelial cells in culture. Microb Pathog. 2002;33(3):109–14.
Lewis LA, Ngampasutadol J, Wallace R, Reid JE, Vogel U, Ram S. The meningococcal vaccine candidate neisserial surface protein A (NspA) binds to factor H and enhances meningococcal resistance to complement. PLoS Pathog. 2010;6(7):e1001027.
Galen JE, Ketley JM, Fasano A, Richardson SH, Wasserman SS, Kaper JB. Role of Vibrio cholerae neuraminidase in the function of cholera toxin. Infect Immun. 1992;60(2):406–15.
Paton JC, Andrew PW, Boulnois GJ, Mitchell TJ. Molecular analysis of the pathogenicity of Streptococcus pneumoniae: the role of pneumococcal proteins. Annu Rev Microbiol. 1993;47:89–115.
Manco S, Hernon F, Yesilkaya H, Paton JC, Andrew PW, Kadioglu A. Pneumococcal neuraminidases A and B both have essential roles during infection of the respiratory tract and sepsis. Infect Immun. 2006;74(7):4014–20.
Kedzierski L, Montgomery J, Curtis J, Handman E. Leucine-rich repeats in host-pathogen interactions. Arch Immunol Ther Exp. 2004;52(2):104–12.
Khan MN, Sharma SK, Filkins LM, Pichichero ME. PcpA of Streptococcus pneumoniae mediates adherence to nasopharyngeal and lung epithelial cells and elicits functional antibodies in humans. Microbes Infect. 2012;14(12):1102–10.
Be NA, Bishai WR, Jain SK. Role of Mycobacterium tuberculosis pknD in the pathogenesis of central nervous system tuberculosis. BMC Microbiol. 2012;12:7.
Gonzalez-Zorn B, Dominguez-Bernal G, Suarez M, Ripio MT, Vega Y, Novella S, Rodriguez A, Chico I, Tierrez A, Vazquez-Boland JA. SmcL, a novel membrane-damaging virulence factor in Listeria. Int J Med Microbiol. 2000;290(4–5):369–74.
Gonzalez-Zorn B, Dominguez-Bernal G, Suarez M, Ripio MT, Vega Y, Novella S, Vazquez-Boland JA. The smcL gene of Listeria ivanovii encodes a sphingomyelinase C that mediates bacterial escape from the phagocytic vacuole. Mol Microbiol. 1999;33(3):510–23.
Huseby M, Shi K, Brown CK, Digre J, Mengistu F, Seo KS, Bohach GA, Schlievert PM, Ohlendorf DH, Earhart CA. Structure and biological activities of beta toxin from Staphylococcus aureus. J Bacteriol. 2007;189(23):8719–26.
Jinadasa RN, Bloom SE, Weiss RS, Duhamel GE. Cytolethal distending toxin: a conserved bacterial genotoxin that blocks cell cycle progression, leading to apoptosis of a broad range of mammalian cell lineages. Microbiology. 2011;157(7):1851–75.
Hurst MR, Jones SA, Binglin T, Harper LA, Jackson TA, Glare TR. The main virulence determinant of Yersinia entomophaga MH96 is a broad-host-range toxin complex active against insects. J Bacteriol. 2011;193(8):1966–80.
Barison N, Cendron L, Loconte V, Proctor EA, Dokholyan NV, Zanotti G. Protein HP1028 from the human pathogen Helicobacter pylori belongs to the lipocalin family. Acta Crystallogr D Biol Crystallogr. 2013;69(8):1387–94.
Baldwin DN, Shepherd B, Kraemer P, Hall MK, Sycuro LK, Pinto-Santini DM, Salama NR. Identification of Helicobacter pylori genes that contribute to stomach colonization. Infect Immun. 2007;75(2):1005–16.
Hsieh PF, Hsu CR, Chen CT, Lin TL, Wang JT. The Klebsiella pneumoniae YfgL (BamB) lipoprotein contributes to outer membrane protein biogenesis, type-1 fimbriae expression, anti-phagocytosis, and in vivo virulence. Virulence. 2016;7(5):587–601.
Weirich J, Brautigam C, Muhlenkamp M, Franz-Wachtel M, Macek B, Meuskens I, Skurnik M, Leskinen K, Bohn E, Autenrieth I, et al. Identifying components required for OMP biogenesis as novel targets for antiinfective drugs. Virulence. 2017;8:1–20.
Misra R, Stikeleather R, Gabriele R. In vivo roles of BamA, BamB and BamD in the biogenesis of BamA, a core protein of the beta-barrel assembly machine of Escherichia coli. J Mol Biol. 2015;427(5):1061–74.
Bartra SS, Styer KL, O'Bryant DM, Nilles ML, Hinnebusch BJ, Aballay A, Plano GV. Resistance of Yersinia pestis to complement-dependent killing is mediated by the Ail outer membrane protein. Infect Immun. 2008;76(2):612–22.
Kolodziejek AM, Sinclair DJ, Seo KS, Schnider DR, Deobald CF, Rohde HN, Viall AK, Minnich SS, Hovde CJ, Minnich SA, et al. Phenotypic characterization of OmpX, an ail homologue of Yersinia pestis KIM. Microbiology. 2007;153(Pt 9):2941–51.
Felek S, Krukonis ES. The Yersinia pestis ail protein mediates binding and Yop delivery to host cells required for plague virulence. Infect Immun. 2009;77(2):825–36.
Wilson MM, Bernstein HD. Surface-exposed lipoproteins: an emerging secretion phenomenon in gram-negative bacteria. Trends Microbiol. 2016;24(3):198–208.
Tanaka SY, Narita S, Tokuda H. Characterization of the Pseudomonas aeruginosa Lol system as a lipoprotein sorting mechanism. J Biol Chem. 2007;282(18):13379–84.
Gehring AJ, Dobos KM, Belisle JT, Harding CV, Boom WH. Mycobacterium tuberculosis LprG (Rv1411c): a novel TLR-2 ligand that inhibits human macrophage class II MHC antigen processing. J Immunol. 2004;173(4):2660–8.
Thomassin JL, He X, Thomas NA. Role of EscU auto-cleavage in promoting type III effector translocation into host cells by enteropathogenic Escherichia coli. BMC Microbiol. 2011;11:205.
Venkatesan MM, Buysse JM, Oaks EV. Surface presentation of Shigella flexneri invasion plasmid antigens requires the products of the spa locus. J Bacteriol. 1992;174(6):1990–2001.
Wandersman C, Delepelaire P. TolC, an Escherichia coli outer membrane protein required for hemolysin secretion. Proc Natl Acad Sci. 1990;87(12):4776–80.
Yamanaka H, Nomura T, Fujii Y, Okamoto K. Need for TolC, an Escherichia coli outer membrane protein, in the secretion of heat-stable enterotoxin I across the outer membrane. Microb Pathog. 1998;25(3):111–20.
Zgurskaya HI, Nikaido H. Multidrug resistance mechanisms: drug efflux across two membranes. Mol Microbiol. 2000;37(2):219–25.
Zgurskaya HI, Krishnamoorthy G, Ntreh A, Lu S. Mechanism and function of the outer membrane channel TolC in multidrug resistance and physiology of Enterobacteria. Front Microbiol. 2011;2:189.
This study was funded by Public Health Service grant AI-051334 from the National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health. The funding body had no role in the design of the study or in the collection, analysis, and interpretation of data, or in the writing of the manuscript.
Availability of data and materials
All data generated or analyzed during this study are included in this published article and its supplementary information files.
Ethics approval and consent to participate
The authors declare that they have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Table S1 (Part 1). Raw unedited Phyre2 data corresponding to 19,560 templates used for T. pallidum whole proteome modeling. (XLSX) Part 2. Raw unedited Phyre2 data corresponding to 780 T. pallidum proteins modeled with high confidence (≥90% confidence; ≥10 alignment coverage). (XLSX 3537 kb)
Table S2. T. pallidum proteins (N = 150) modeled by Phyre2 with confidence scores below 90% and annotated in the published T. pallidum proteome as hypothetical/putative membrane proteins. (XLSX 34 kb)
Table S3. T. pallidum proteins (N = 46) modeled by Phyre2 with confidence scores below 90% with functional annotations in the published proteome. (XLSX 25 kb)
Table S4. Comparison of T. pallidum primary and tertiary structure-based functional annotations using only the top-ranked Phyre2 model template for each protein comparison. (XLSX 33 kb)
Table S5. Comparison of T. pallidum primary and tertiary structure-based functional annotations using all confident Phyre2 model templates for each protein comparison. (XLSX 34 kb)
Table S6. Top-ranking Phyre2 model templates corresponding to all functionally-unannotated T. pallidum proteins (N = 175). (XLSX 40 kb)
Table S7. Functionally-unannotated T. pallidum proteins (N = 28) with top-ranking templates modeled against structures classified by the PDB as “unknown function”. (XLSX 13 kb)
Table S8. Functionally-unannotated T. pallidum proteins [N = 20] with Phyre2-based predicted functions using all confident model templates. (XLSX 30 kb)
Table S9. Functionally-unannotated T. pallidum proteins [N = 8] modeled exclusively against PDB structures of unknown function. (XLSX 14 kb)
Table S10. Phyre2 Modeling of T. pallidum proteins previously annotated as putative virulence factors. (XLSX 17 kb)
Table S11. Comparison of potential virulence factors identified by comparative genomics and Phyre2 modeling. (DOCX 13 kb)
Table S12. Genome-annotated T. pallidum proteins with potential novel virulence-related functions identified by Phyre2 modeling. (XLSX 11 kb)
Table S13. Proteins of unknown function with potential roles in T. pallidum virulence. (XLSX 21 kb)
About this article
Cite this article
Houston, S., Lithgow, K.V., Osbak, K.K. et al. Functional insights from proteome-wide structural modeling of Treponema pallidum subspecies pallidum, the causative agent of syphilis. BMC Struct Biol 18, 7 (2018). https://doi.org/10.1186/s12900-018-0086-3