- Research article
- Open Access
Crystal structures from the Plasmodium peroxiredoxins: new insights into oligomerization and product binding
BMC Structural Biology volume 12, Article number: 2 (2012)
Plasmodium falciparum is the protozoan parasite primarily responsible for more than one million malarial deaths, annually, and is developing resistance to current therapies. Throughout its lifespan, the parasite is subjected to oxidative attack, so Plasmodium antioxidant defences are essential for its survival and are targets for disease control.
To further understand the molecular aspects of the Plasmodium redox system, we solved 4 structures of Plasmodium peroxiredoxins (Prx). Our study has confirmed Pv Trx-Px1 to be a hydrogen peroxide (H2O2)-sensitive peroxiredoxin. We have identified and characterized the novel toroid octameric oligomer of Py Trx-Px1, which may be attributed to the interplay of several factors including: (1) the orientation of the conserved surface/buried arginine of the NNLA(I/L)GRS-loop; and (2) the C-terminal tail positioning (also associated with the aforementioned conserved loop) which facilitates the intermolecular hydrogen bond between dimers (in an A-C fashion). In addition, a notable feature of the disulfide bonds in some of the Prx crystal structures is discussed. Finally, insight into the latter stages of the peroxiredoxin reaction coordinate is gained. Our structure of Py Prx6 is not only in the sulfinic acid (RSO2H) form, but it is also with glycerol bound in a way (not previously observed) indicative of product binding.
The structural characterization of Plasmodium peroxiredoxins provided herein provides insight into their oligomerization and product binding which may facilitate the targeting of these antioxidant defences. Although the structural basis for the octameric oligomerization is further understood, the results yield more questions about the biological implications of the peroxiredoxin oligomerization, as multiple toroid configurations are now known. The crystal structure depicting the product bound active site gives insight into the overoxidation of the active site and allows further characterization of the leaving group chemistry.
There are at least 500 million clinical episodes of malaria annually with more than a million Africans dying each year, most of whom are children under 5 years of age . The causative agent for the most lethal form of malaria is a protozoan parasite, Plasmodium falciparum, while P. vivax causes a less severe form, P. knowlesi is responsible for macaque malaria (but it can also infect humans [2, 3]), and P. yoelii and berghei infect rodents. Plasmodium parasites are frequently subject to oxidative attack, for example, in the erythrocyte from H2O2 release during heme metabolism and from NO and reactive oxygen species (ROS) generation during the host immune response [4, 5]. In addition, oxidative stress is sustained during the sexual maturation of the parasite within the Anopheles mosquito midgut and salivary gland prior to transmission [6, 7]. As such, Plasmodium antioxidant defences are essential to its survival, and thus are expected to be targets for the effective control of the disease [8, 9].
Interestingly, neither the Plasmodium parasites nor the trypanosomes contain a catalase or a selenocysteine-containing glutathione peroxidase (GPx), which are enzymes notably efficient for the detoxification of hydroperoxides [10, 11]. Plasmodium does however possess 2 superoxide dismutases, 6 proteins homologous to thiol-dependent peroxidases, and a glutathione-S-transferase (GST). The GST has only weak GSH peroxidase activity, but it might contribute significantly to the antioxidant capacity of the parasite due to its high concentration . Of those homologous to the thiol-dependent peroxidases, there is the GPx-like thioredoxin peroxidase, which is a non-selenocysteine GPx known to be significantly less active than its selenium homologue . The 5 remaining thiol-dependent peroxidase homologues identified in Plasmodium include thioredoxin peroxiredoxin 1 and 2 (Trx-Px1 and Trx-Px2) from the peroxiredoxin subfamily Prx1, a 1-Cys peroxiredoxin (1-Cys Prx) from the Prx6 subfamily, antioxidant protein (AOP) from subfamily Prx5, and a very recently characterized nuclear peroxiredoxin (Pf nPRx)  (Table 1). Interestingly, peroxiredoxins have recently been implicated in a different role, namely as a non-transcriptional rhythmic marker, indicative of the circadian clock . Various strategies have been used to classify the members of the peroxiredoxin family including a phylogenetic tree analysis that categorizes them into 6 subfamilies (Prx1, Prx6, Prx5, Trx-Px, BCP, and AhpE), each of which may include the mechanistically distinct 1-Cys and 2-Cys peroxiredoxins [20, 21].
All peroxiredoxins contain a conserved cysteine residue at the N-terminus that is referred to as the peroxidatic cysteine (CP). During catalysis, it is oxidized by the ROS substrate (generally H2O2 or an alkyl hydroperoxide) to sulfenic acid (Cys-S-OH). Typical 2-Cys Prx contain 2 conserved cysteines, including the CP and a C-terminal cysteine (termed the resolving Cys (CR)). During catalysis, the CP sulfenic acid reacts with the CR of the adjacent monomer to form the intermolecular disulfide of the homodimer that is subsequently reduced by another (undetermined) thiol. In 1-Cys Prx, the CP sulfenic acid is directly reduced by an unidentified redox partner.
Building on the dimer formation, the 2-Cys Prx enzymes organize themselves into higher order oligomers, such as decamers, which have higher peroxidase activity. Formation of the higher order oligiomers is dependent on the redox state of CP (and CR), as well as other factors [22, 23]. Both Trx-Px1 and Trx-Px2 have been identified as typical 2-Cys Prx enzymes; and a crystal structure of P. falciparum Trx-Px2 (PDB ID: 2C0D) has been published . Distinguishing these two Plasmodium thioredoxin peroxidases is their cellular location, as Trx-Px1 is predicted to be cytosolic and Trx-Px2 has a mitochondrial targeting sequence . Both features were recently confirmed . Like the Plasmodium 1-Cys Prx from subfamily Prx6, AOP is also a 1-Cys Prx; and the P. falciparum AOP structure has been solved (PDB ID: 1XIY) . AOP is thought to be an apicoplast enzyme due to its N-terminal signal motif, while the other Plasmodium 1-Cys Prx is cytosolic. Both predictions were recently confirmed experimentally . Prx enzymes are highly expressed in Plasmodium (0.5% of cellular protein) and have been predicted from competitive kinetic analysis with human cells to be responsible for the reduction of 90% of mitochondrial H2O2 and nearly 100% of cytoplasmic H2O2.
In order to further the understanding of the molecular details of the Plasmodium redox system, we solved the crystal structures of Trx-Px1 from P. vivax (Pv Trx-Px1) in the reduced and oxidized forms, Trx-Px1 from P. yoelii (Py Trx-Px1) in the oxidized form, and a 1-Cys Prx from P. yoelii (termed Py Prx6, herein) with CP oxidized to the sulfinic acid and with glycerol bound within the active site pocket. In addition, we have structurally confirmed and characterized the Pv Trx-Px1 as a H2O2-sensitive peroxiredoxin; Py Trx-Px1 as forming an octamer (instead of the typical decamer and dodecamer arrangements); and Py Prx6 as a product bound complex revealing some interesting features of these enzymes.
Expression and initial characterization of Trx-Px1 from P. falciparum, P. vivax, P. yoelii, and P. knowlesi and Prx6 from P. yoelii
Constructs of the Trx-Px1 enzymes from P. falciparum, P. vivax, P. yoelii, and P. knowlesi were expressed and purified as described previously . Py Prx6 was also expressed in Studier auto-induction media . All were full length constructs except Py Trx-Px1 which was also expressed for crystallization with a 6-residue truncation at the N-terminus. According to our mass spectroscopic analysis, all of our purified Trx-Px1 enzymes were disulfide-linked dimers (Table 2). As verified by mass spectroscopy, each of the 4 purified Trx-Px1 enzymes could be completely reduced using 20 mM dithiothreitol (Table 2).
By analytical gel filtration, all of the purified Trx-Px1 constructs (oxidized form and at high μM concentrations) eluted primarily as the higher order oligomer (i.e. octamer or decamer or dodecamer) (data not shown). Oligomerization has been shown to be dependent on numerous factors including ionic strength, pH, concentration of divalent metals, and most importantly redox state . In our gel filtration, a small amount of presumably aggregated protein was followed by the oligomeric protein around (corresponding to a calculated mass of 272 or 314 kDa). In the case of Pf Trx-Px1, some presumably dimeric protein was also observed, which was not noticed in previous gel filtration analyses of Pf Trx-Px1 by Akerman and coworkers. . These authors only observed higher order oligomers corresponding to 400 and 250 kDa for Pf Trx-Px-1 and further detected the (α2)5 quaternary form by electron microscopy.
Py Prx6 was expressed in two ways: Py Prx6 expressed from our typical protocol was purified in the reduced form according to mass spectroscopy (27189.1 Da) without addition of exogenous reducing agents (with the expected molecular weight after cleavage of the N-terminal Met weight being 27188.2 Da), while Py Prx6 expressed from Studier auto-induction media was purified in the sulfinic acid form (see below). From a calibrated gel filtration column, the enzyme eluted at 220 mL (corresponding to a calculated gel filtration mass of 62 kDa) which is consistent with the expected behaviour of a dimer.
Crystal structures of Trx-Px1 from P. vivax and P. yoelii
The crystal structures of P. vivax Trx-Px1 were solved in both the reduced (Pv Trx-Px1_red, PDB ID: 2I81) and oxidized forms (Pv Trx-Px1_ox, PDB ID: 2H66) at 2.45 Å and 2.5 Å, respectively (Figure 1). Aside from these 2 structures, to date only rat and Salmonella typhimurium Prx1 subfamily structures have been solved in both fully reduced and oxidized (as the disulfide) redox states . In comparison to other solved structures, Pv Trx-Px1 is most similar (52% sequence identity) to Pf Trx-Px2 . It has 85% sequence identity with Pf Trx-Px1 and 47% identity to its closest human orthologues. Herein, the oxidized form of Py Trx-Px1 has also been solved to 2.3 Å (PDB ID: 2H01) (Figure 1C).
The structures of each (Pv Trx-Px1_red, Pv Trx-Px1_ox, and Py Trx-Px1_ox) contain the typical thioredoxin-fold found in known peroxiredoxins. There is a central 7-stranded β-sheet comprised of β2-β1-β5-β4-β3-β6-β7 with β1 and β6 running anti-parallel relative to the other strands. This β-sheet is sandwiched by α1 and α4 on one side and α2, α3, and α5 on the other side. The root mean square deviation (rmsd) between Pv Trx-Px1_ox, and Py Trx-Px1_ox is 0.91 Å when superimposing over the monomer encompassing Cα's from residues 2 to 177. The remaining residues of the C-terminus are disordered (from 178 to 195) in the Pv Trx-Px1_ox structure.
In Pv Trx-Px1_red structure, the CP residue (Cys50 for P. vivax) is located at the first turn of helix α2 at the end of a narrow accessible channel formed by a loop-helix motif and surrounded by 3 conserved residues Pro43, Thr47, and Arg125 (Figure 2A, red/dark grey). The pyrrolidine ring of Pro43 limits solvent accessibility and protects the reactive cysteinyl sulfenic acid from further oxidation during catalysis. The distance between Cys50 and the corresponding CR thiol from its dimeric partner is 13.5 Å in the reduced form. In both the oxidized structures of Pv Trx-Px1_ox and Py Trx-Px1_ox, the α2 helix is locally unfolded (LU) around the CP, such that the α2 helix begins after the CP with Ser52 (coloured in orange in Figure 2A for Pv and Py: Pv Trx-Px1_ox) or Ser46 (Figure 2B for Py Trx-Px1_ox). Some conserved residues that form the active site in the reduced structure are in the same positions and orientations in both the oxidized structures (Pro43, Thr47, and Arg125 for PvTrx-Px1, Figure 2A, orange/light grey and Pro37, Thr41, and Arg119 for Py Trx-Px1, Figure 2B). The CP has rotated to the surface as part of a highly exposed loop; and SP (sulfur of CP) is engaged in a disulfide bond with SR (sulfur of CR) of the enzyme's dimeric partner forming a domain swapped homodimer.
Pv Trx-Px1 and Py Trx-Px1 are H2O2-sensitive peroxiredoxins
The 3 sequence motifs that define the H2O2-sensitive peroxiredoxins include: (1) the conserved loop-helix from Pro43 to Glu53 surrounding the CP; (2) a 310 helix-loop from Pro89 to Ile98; and (3) the 29 C-terminal residues from Gly167 including CR and conserved bulky residues . The loop-helix is completely conserved among human, rat, and Plasmodium peroxiredoxins. However, the Plasmodium enzymes are a slight variation of 310 helix-loop and C-terminal tail motifs with sequences including 93GGIG96 and 191YL192, respectively, instead of the typical GGLG and YF (Figure 3A). Upon addition of 500 μM to 5 mM H2O2 to reduced (by DTT) Pf Trx-Px1, Pv Trx-Px1, Pk Trx-Px1, or Py Trx-Px1 enzymes, additions of 2 and 3 oxygen atoms were observed by mass spectroscopy, confirming that these enzymes are H2O2-sensitive. Structurally, the first loop-helix and the C-terminal arm of P. falciparum were predicted by modelling to undergo the same structural rearrangement during catalysis as the mammalian peroxiredoxins . Figure 3B, 3C illustrate the structural changes that Pv Trx-Px1 does indeed undergo during catalysis further supporting its characterization as a H2O2-sensitive peroxiredoxin. Although predicted by Kawazu , to be fluid (i.e. structurally disordered) from Pro171 immediately following CP, the C-terminal tail is an ordered loop from Pro171 to Gly177 in the Pv Trx-Px1_ox structure. The Py Trx-Px1_ox structure also shows a similar arrangement of conserved residues, a 310 helix-loop motif at 90PLSQGGIGNI98, and a C-terminal tail bearing a 191YL192 motif (nearly identical to Pv Trx-Px1 that folds upon reduction into a loop followed by an α-helix), so it is also expected to be a H2O2-sensitive peroxiredoxin (Figure 2B and 3A). The conserved 191YL192 motif that is located on the α-helix close to the surface stabilizes the full-folded conformation. This motif therefore slows the resolution reaction and allows overoxidation by reaction with a second equivalent of peroxide. In contrast robust peroxiredoxins do not have residues protecting the CP and are quickly oxidized to the disulfide .
Disulfide bonds in the Pv Trx-Px1_ox and Py Trx-Px1_ox structures
Upon examination of each dimer of Pv Trx-Px1_ox decamer ((α2)5 oligomer) (Figures 1A and 4A), only 4 of a possible 10 disulfide bonds are clearly defined in the crystal structure. There is inadequate density to define the C-terminal tail from around the CR for the remaining residues, so that for several of the monomers only the side chain of CP but not that of CR is visible. In the case of the Py Trx-Px1_ox octamer ((α2)4 oligomer) (Figures 1C and 4B) (for which the data was collected at a home source) the Cys-SP to Cys-SR distances measure ~3 Å (notably, bond lengths at the resolution of these structures are derived from a combination of x-ray data and chemical constraints). There are no reports indicating that disulfide bonds are labile under the conditions used in our Py Trx-Px1 experiments. As expected, the C-terminal tails of Pv Trx-Px1 and Py Trx-Px1 have higher B-factors than the other parts of the molecule, indicative of a more fluid region and also of the apparent absence of detectable disulfide bonds in portions of the Pv Trx-Px1 structure and the distortion of the disulfide bond length in the Py Trx-Px1 structure. In previously published crystal structures of oxidized 2-Cys Prx, the Cys-SP to Cys-SR distance is also longer than expected for a disulfide bond (as the typical bond length is 2.05 for a disulfide). For example in the structure of a 2-Cys Prx from Helicobacter pylori (Hp AhpC) (PDB ID: 1ZOF), the Cys-SP to Cys-SR distances measurements range from 2.0 to 3.2 Å and in a P. falciparum 2-Cys Prx (Pf Trx-Px2) (PDB ID: 2C0D) one of the disulfides is shown in two different orientations (2.0-2.2 Å) indicative of the structural flexibility of these structures, while the other measures at 2.6 Å. Our analysis using mass spectroscopy (already discussed) and the overall structural configurations (i.e. local unfolding about CP and at the C-terminus) both support Pv Trx-Px1_ox and Py Trx-Px1_ox being in the oxidized form making these long Cys-SP to Cys-SR distances not easily accounted for, yet prevalent in the Prx1 subfamily.
Oligomeric organization of Pv Trx-Px1 and Py Trx-Px1
Oligomeric peroxiredoxins are formed via at least 2 types of interactions: (1) B-type interactions where edge to edge associations of β7 strands of the central sheet meet to extend it into a 14-stranded β-sheet; and (2) A-type where the interface is a tip to tip association centred on helices α4 and α5 packing against helices α4 and α5 of the other chain . The dimer interface of the Pv Trx-Px1_ox and Py Trx-Px1_ox (i.e. where the interactions resulting at the surfaces formed by the disulfide bond) is termed the B-type interaction face. Accordingly, the same dimeric unit is expected in the reduced structures. These dimers then associate via A-type interactions to form the higher order oligomers, which are typically decameric and dodecameric. Pv Trx-Px1_ox and Pv Trx-Px1_red arrange in a decameric fashion (according to our crystal structure), which from a survey of peroxiredoxin structures deposited to the PDB is the most common oligomerization. According to our results (namely a crystal structure and the gel filtration elution times characteristic of the higher order oligomer and not a dimer), the toroid Py Trx-Px1 structure is unique in that it is octameric (Figure 4B). Pv Trx-Px1_ox and Pv Trx-Px1_red decamers have internal diameters of ~58 Å, while Py Trx-Px1_ox octameric diameter is correspondingly smaller at ~50 Å. Li et al. reportedly solved the structure of another octameric peroxiredoxin (Mycobacterium tuberculosis AhpE), but this has been disputed as simply a crystallographic artifact, as predominately dimers were observed in gel filtration and the octameric interface is not extensive and does not involve the typical interfaces [32, 33]. It could be argued that the octameric arrangement of Py Trx-Px1 is a result of the crystal packing of Py Trx-Px1_ox dimers; however, no dimers were observed in the gel filtration of the Py Trx-Px1_ox sample which was done at a high μM concentration comparable to crystallization experiments. Interestingly, there is a hydrogen bonding interaction in the Py Trx-Px1 structure between distant monomers and more specifically between adjacent dimers, where the C-terminal tail of one monomer crosses its dimeric partner to hydrogen bond to next monomer. Contributing to the stability of the octamer, the side chain of Lys81 is within hydrogen bonding distance (3.2 Å) of the backbone carbonyl of Lys172 (Figure 4C). Indeed, there are interactions between each of the pairs A-C, B-D, C-E, D-F, E-G, F-H, G-A, and H-B, as one would expect from symmetry (Figure 4D). Although these 2 residues are conserved in Pv Trx-Px1, there is no similar interaction in the Pv Trx-Px1_ox or Pv Trx-Px1_red structures (where the corresponding residues are Lys87 and Gly177). There is 83% sequence identity between the P. yoelii and P. vivax Trx-Px1 enzymes, so the differences in oligomeric state were not predictable. Previous reports have identified alterations of the B-type interface as conferring the different orders of oligomeric state, while the A-type interface remains constant . An alignment of the Cα's of a monomer from the decameric Pv Trx-Px1_ox with a monomer from octameric Py Trx-Px1_ox (rmsd = 0.573 Å) is shown in Figure 4E, F to illustrate the overlap of each accompanying monomer in the A-type interface and B-type interface, respectively. In Figure 4E, the corresponding A-type interface dimer is shown for the octamer and decamer which visually appears to preserve the overlap for their respective dimeric partners. Indeed, a structural alignment of the A-type dimers between the octamer and decamer gives a rmsd of 1.065 Å. Figure 4F shows the same monomers aligned, but in this case, their respective B-type interface with the dimeric partner from the opposite side shown. Here, one can see that the overlap is poor which is also reflected as a much larger rmsd of 3.137 Å for the alignment of octameric and decameric B-type dimeric partners. In order to more directly compare the interfaces, each monomer is simplified to the 2 Cα's of conserved leucine residues at its core, for example, Leu 102 and Leu139 from Pv Trx-Px1and corresponding Leu96 and Leu133 from Py Trx-Px1 (Figure 5A, B, respectively). An analysis of the changes between the Py Trx-Px1 octamer and the Pv Trx-Px1 decamer was undertaken using these conserved core residues as a representation of each monomer. Indeed, the model is validated, as the distance between the selected leucine residues (termed core length) is conserved throughout each of the structures and is similar between the two structures (Figure 5D). The orientation (termed angle between the dimers) and distance between the dimers within the oligomer is also conserved between the Py Trx-Px1 octamer and Pv Trx-Px1 decamer (Figure 5D). On the other hand, the orientation (termed angle between monomers) and distance between the monomers of the dimer (termed core distance) is dramatically different between the octamer and decamer. The observation that the different oligomerization is attributable to the interface within the dimer suggests that the oligomerization is not an artifact of crystallization and that the dimer itself is unique at least in terms of its B-type interface.
Analysis of the structures of Py Trx-Px1 and Pv Trx-Px1 identifies several key points of difference between the structures that may account for difference in oligomerization. First of all, the presence of a hydrogen bond between the dimers of Py Trx-Px1 (and not Pv Trx-Prx1) was already discussed (Figure 4C, D). Secondly, the C-terminal tails have different orientations, such that they are binding at different positions on the surface of their respective dimeric partners (Figure 6). The tails are bound by an intermolecular disulfide between CP and CR, as well as a series of hydrophobic interactions. The different binding orientations are linked to the orientation of the side chain of a conserved arginine (Arg142 and Arg148 for Py Trx-Px1 and Pv Trx-Px1, respectively) (Figure 6A, B). In the case of Pv Trx-Px1, the arginine is buried and the C-terminal tail adopts the typical binding pattern on the surface of its respective dimeric partner (Figure 6D). As opposed to Py Trx-Px1 where the equivalent arginine side chain is at the surface of the protein obstructing the typical pathway, such that the C-terminal tail adopts a different position at the surface of its partner (Figure 6C). Using the NCBI Molecular Modeling Database http://www.ncbi.nlm.nih.gov/Structure/MMDB/mmdb.shtml, a search was performed to identify 3D structures to similar Py Trx-Px1. A manual inspection of each the 2-Cys peroxiredoxins of the 143 low redundancy hits (from the 1532 total hits) showed no similarity in orientation to the Py Trx-Px1 C-terminal tail, as expected as no other toroid octameric peroxiredoxins are known to date. A second point of differentiation between the two structures is a loop with the conserved sequence 142NNLA(I/L)GRS149 (numbering from Pv Trx-Px1 (PDB: 2H66)) that connects α5 and β7 (β-sheet involved in the peroxiredoxin B-type interface) and contains the afore mentioned buried/surface Arg. The loops from each structure adopt different conformations that put Arg142 at the surface for Py Trx-Px1 and the corresponding Arg148 buried for Pv Trx-Px1. Although the Pv Trx-Px1 structure is from a full-length construct, only a structure with an N-terminal truncation of 6 residues crystallized sufficiently well for data collection in the case Py Trx-Px1. Although both constructs bear a N-terminal His6-tag, the tag could only be cleaved from the Pv Trx-Px1 construct. Even after several days at room temperature (where typical conditions require an overnight reaction at 4°C), the His6-tag remained intact on Py Trx-Px1. Although the tag is partially visible in Py Trx_Px1 case(-3AFQG1 from PDB: 2H01), it is not close enough to the conserved loop in both cases to affect its orientation. Further studies will determine what roles the structural features identified herein (i.e. the hydrogen bond between the dimers, the N-terminal tail, the surface/buried arginine, the NNLA(I/L)GRS-loop, and the C-terminal tail) play in the stoichiometry of oligomerization of these Trx-Px1 enzymes.
Crystal structure of Py Prx6 with product bound
The Py Prx6-SO2H crystal structure has been solved at 2.3 Å (PDB ID: 3TB2). It is 47% identical to its closest human orthologue and shares 76% sequence identity with its P. falciparum orthologue. The closest available structure by sequence (at 48% sequence identity) is Arenicola marina peroxiredoxin 6 which was further identified as a 2-Cys Prx , but it also shares 47% sequence identity with a solved structure for a human 1-Cys Prx . Overall, the human (PDB ID: 1PRX) and P. yoelii structures are very similar with a core thioredoxin-fold and a C-terminal domain connected by both an extended helix α5 and a loop. The core rmsd is 1.38A for 401 aligned residues in both structures. The C-terminal domain of Py Prx6 that comprised of 3 β-strands and a α-helix is larger than the C-terminal domain from the Prx1 subfamily (Figure 1D). This domain from each monomer extends over the other forming a domain swapped dimer.
The active site CP (Cys47) is located at the bottom of a narrow pocket (~4 Å by ~7 Å) at the end of helix α2. There are 2 additional densities in each of the 4 active site pockets of the asymmetric unit (Figure 7) which are best filled by a glycerol molecule (from the purification buffer used which contained 5% glycerol) and fitting the CP residues to their sulfinic acid derivatives (CP-SO2H). Previously, the CP has been structurally characterized in the whole range of oxidation states (including CP-SH, CP-SOH, SP-SO2H, CP-SO3H, and CP-SS-CR) ; and our data agrees with those sulfinic acid structures previously studied. As well, nearly 20 peroxiredoxin structures have either substrate or what has been termed substrate analogue bound in their active site pockets, including H2O2, benzoate, acetate, dithiothreitol (oxidized), ethylene glycol, glycerol, sulfate, citrate, and formate .
In order to understand the reaction mechanism, our focus is on the comparison of structures with substrate (H2O2) or glycerol bound. The binding of H2O2 to Aeropyrum pernix Tpx (thiol peroxidase) has been structural characterized (PDB: 3A2W and 3A2V) . Further work on these structures and comparisons to other ligand bound peroxiredoxin structures previously showed that oxygen atoms of the ligands overlap with the proximal (relative to CP) oxygen atom (OA) and/or the distal oxygen atom (OB) of bound H2O2. Interestingly, the glycerol molecule found in these structures can adopt all three possibilities: (1) in one monomer of Ap Trx (PDB: 3A2W) it is found with a single oxygen atom overlapping OA (Figure 7C, pink); (2) in another monomer of Ap Trx (PDB: 3A2W), it is found to overlap with both OA and OB (Figure 7C, pink); and (3) reported for the first time, in our structure of Py Prx6, the glycerol is observed to overlay with only OB (Figure 7C, purple). Previously, only 2 anionic ligands (sulfate and citrate) were observed to occupy OB alone (see PDB: 1TP9 and 3DRN) . Despite variations in the backbone orientation of the glycerol in our structure (which is also seen in the Ap Trx glycerol bound chains and presumably due to its conformational flexibility), the binding of all 4 glycerol molecules shows that each binds with the terminal hydroxyl in a similar position overlapping with OB site (Figure 7B). Oxygen atoms at position OA are postulated to be a mimetic for the substrate bound in a Michaelis complex ready for attack by the nucleophilic CP, while oxygen atoms at position OB are indicative of the leaving group (H2O or alcohol) . As such, it can be suggested that the glycerol positioned with its terminal hydroxyl at OB is in a product bound configuration. Although there is variability in the binding of the remainder of the glycerol molecule, the alkyl group is always directed away from the active site pocket and the oxygen of the leaving group is in close proximity to a conserved threonine (Thr44). This arrangement suggests that it may be the proton donor, although others have suggested that this threonine functions as a hydrogen bond acceptor as it deprotonates the incoming substrate for attack by the CP thiolate and that bulk solvent is responsible for the protonation of the leaving group . Therefore, the active site pocket is adapted to accommodate different substrates (and thus products), which is exemplified through the structural flexibility exhibited in the product bound glycerol shown herein. As well, the OB position appears to be designated for the oxygen of the leaving group and is well positioned for protonation by a conserved threonine.
The conserved residues of the CP loop comprised of 40PxxxxTxxCP47 and conserved Arg127 (numbering refers to Py Prx6) are implicated in catalysis (Figure 8). For the conserved arginine in both Py Prx6 (Arg127) and ApTrx•H2O2 structures, it adopts position I, typical of Prx6, which bears a conserved arginine (Arg152) and glutamate (Glu50) (or possibly a glutamine or histadine) and supports the positioning by a hydrogen bonding network, as previously described . The active site geometry is fully folded (FF) and is virtually identical to that of the Ap Trx•H2O2. As was recently described, the nucleophilic CP (as activated by a main chain amide N-H and the conserved arginine guanidinium) is expected to act as a thiolate and attack the substrate in an SN2 fashion . These hydrogen bonds, as well as those secondary ones from the backbone carbonyls and the glutamic acid/second conserved arginine, surrounding the CP are preserved between the Ap Trx x•H2O2 and the Py Prx6•glycerol structures suggesting that the sulfinic acid form may be activated, and thus sufficiently nucleophilic (similar to its full reduced state) within the active site to undergo a further reaction with substrate. This would result in a subsequent oxidation of the active site to the sulfonic acid. Indeed, this form of the CP has been structural characterized in other cases (PDB: 2CV4, 2NVL, and 1XIY). The conserved proline serves as a barrier to solvent, while the main chain of the CP loop provides hydrogen bonds to the CP and the conserved threonine. Although at a reduced efficiency owing to the relative activity of an oxidized thiol relative to a thiolate, the preservation of the hydrogen bonding network about the active site cysteine, indicates that sequential H2O2 reductions are possible.
Plasmodium lacks the two major antioxidant enzymes of eukaryotic cells, namely glutathione peroxidase and catalase . As such, the parasite is likely to rely on the peroxiredoxin family to control peroxide production (as well as other reactive oxygen and nitrogen species) during critical stages of its lifecycle, for example during erythrocyte invasion when heme metabolism and immune response pathways ensue. During the trophozoite stage (feeding period), Prx enzymes accounts for 0.5% of the total expressed protein . One recent study suggests that Pf nPrx, the P. falciparium nuclear peroxiredoxin might be essential in the erthyrocyte stage, as neither P. falciparum or P. berghei knock-out lines could be generated despite several attempts and success with generating tagged nPrx-GFP fusion cell lines . Analysis of the growth of a P. berghei Trx-Px1 knockout also suggests that Trx-Px1 is not essential for growth, but P. falciparum and P. bergei show differences in their lifecycles . There is a possibility that Trx-Px1 is essential during the asexual growth of P. falciparum. Expression of Pf 1-Cys Prx is elevated during the trophozoite and early schizont stages (when the parasites are maturing during the liver phase) suggesting that this subfamily detoxifies ROS, like those released during heme metabolism . Despite the important roles of peroxiredoxins, whether inhibitors targeting Plasmodium peroxiredoxins will lead to parasite death remains to be determined.
Structural characterization of the 2-Cys Prx enzymes has shown that the C-terminal tail (referred to as the CR) is essential for stabilizing the octameric/decameric arrangement of peroxiredoxins. When Trx-Px1 (or another 2-Cys Prx) is oxidized to the disulfide form, the CR loop is unfolded; and structural support of the octamer/decamer/dodecamer interfaces are weakened giving rise to dimer formation (as seen in part when our oxidized Pf Trx-Px1 is run on a gel filtration column). When the CR loop is folded as in the reduced form, the higher order oligomer is favoured because of increased stabilization for the B-type interface which supports oligomerization. The dramatic rearrangement of the C-terminal tail and ensuing changes in stability are clearly demonstrated in our structures from Py Trx-Px1 and Pv Trx-Px1 (both oxidized and reduced forms). However, the high concentrations of protein used in crystallizing the enzyme (and also apparently during most of our gel filtration experiments) may account for the trapping of the disulfide forms of these peroxiredoxins in their predicted unfavoured octameric/decameric/dodecameric forms. Factors associated with the oligomeric forms primarily include reduction of the active site disulfide, but also, high or low ionic strength, low pH, high magnesium or calcium concentrations, and overoxidation of the peroxidatic cysteine (Cys-SO2H) . At physiologically relevant concentrations, peroxiredoxins can be expected to exist as dimers awaiting reduction; and upon reduction, the catalytic cycle is complete and the reduced peroxiredoxin oligomerizes . With data from the novel octameric configuration of Py Trx-Prx1 studied herein, other specific structural features affecting oligomerization are considered. The molecular basis for octamer formation relies on the hydrogen bond between the dimers which is facilitated by the positioning of the C-terminal tail which in turn rests on interplay between the surface/buried arginine of the NNLA(I/L)GRS-loop and possibly the N-terminal tail. In vivo work directed at the further characterization of the different sizes and configurations of the oligomers will be necessary to fully understand the biological implications.
Aside from being antioxidant proteins, 2-Cys Prx (Prx1) has also been implicated in H2O2-mediated signal transduction. Eukaryotic 2-Cys Prx enzymes are sensitive to oxidative inactivation, while their bacterial orthologues are robust with respect to overoxidation . The Plasmodium 2-Cys Prx enzymes accordingly have the 3 sequence motifs indicative of the H2O2-sensitive peroxiredoxins, and as shown herein are structurally identical with respect to sensitivity to H2O2 to known H2O2-sensitive 2-Cys Prx enzymes, thus allowing low resting levels of H2O2, while permitting higher levels during signal transduction.
The Py Prx6 structure presented herein has greatly enhanced our understanding of the chemistry of the peroxiredoxins. With a product bound configuration, the residues supporting the leaving group are further understood. There is flexibility for the alkyl chain, but the OB position is indeed designated for the oxygen of the leaving group. As well, the retention of hydrogen bonds about the active site thiol (even in an oxidized state) indicates that it is poised for further reaction, albeit at a reduced efficiency owing to the reduced activity of an oxidized thiol relative to a thiolate.
Our structural data and mass spectroscopy confirms that Pv Trx-Px1 is H2O2-sensitive peroxiredoxin. The characterization of the oligomerization of Py Trx-Px1 has identified structural features supporting its novel octameric oligomerization. Previously unreported abnormalities of the disulfide bond measurements in some of the Prx crystal structures are brought to the forefront. Finally, a crystal structure with an alcohol bound and the CP oxidized gives a view to the product bound complex providing insight into leaving group and the susceptibility of some peroxiredoxins to overoxidation. These results enhance our understanding of the structural variations of the peroxiredoxin oligomers and the nature of the catalysis by these remarkable enzymes. Further work will lend insight into the biological implications of the oligomerization and how to exploit the active site features in drug discovery programs.
Cloning, expression, and purification
Full-length P. falciparum Trx-Px1 encoded by PlasmoDB ID: PF14_0368 http://plasmodb.org/plasmo/ was cloned from P. falciparum 3D7 genomic DNA with a His6-tag with an integrated thrombin cleavage site (MGSSHHHHHHSSGLVPR*GS). Full-length P. knowlesi Trx-Px1 encoded by PlasmoDB ID: PKH_126740 was cloned from P. knowlesi H genomic DNA with a His6-tag with an integrated TEV cleavage site (MGSSHHHHHHSSGRENLYFQ*G). Full-length P. vivax protein encoded by PlasmoDB ID:PVX_118545 with an N-terminal His6-tag and TEV cleavage site (as above) was cloned from a P. vivax Salvador I cDNA library (generously provided by Prof. Liwang Cui of Penn State University). Full-length P. yoelii protein encoded by PlasmoDB ID: PY00414 (Trx-Px1) was cloned from P. yoelii 17XNL genomic DNA with an N-terminal His6-tag and integrated TEV protease site (as above). Full-length P. yoelii protein encoded by PlasmoDB ID: PY04285 (Prx6) was cloned from P. yoelii 17XNL genomic DNA with an N-terminal His6tag with different integrated TEV protease site (MGSSHHHHHHSSGRENLYFQ*GHM) and C-terminal addition (GS). All enzymes were expressed and purified according to methods described previously  except for Py Prx6 which was expressed from Studier auto-induction media .
All mass spectra were completed on an Agilent LC-MS-TOF (Model #G1969A) running in positive ion mode and integrated with an Agilent 1100 series HPLC using an Agilent Poroshell 300-SB-C3 column for fast binding/elution desalting. All gel filtration experiments were complete on a AKTA purifier chromatography system (GE Healthcare Life Sciences) equipped with a Superdex S200 gel filtration column that was calibrated with 4 samples (bovine thyroglobulin (670 kDa), bovine γ-globulin (158 kDa), chicken ovalbumin (44 kDa), and horse myoglobin (17 kDa) all from Bio-Rad) in 10 mM HEPES, pH 7.4 and 500 mM NaCl.
Crystallization and structure determination
Pv Trx-Px1_ox with the His6-tag intact was crystallized by mixing 1.5 μL of protein (at a concentration of 8 mg/mL in a buffer of 10 mM HEPES, pH 7.5, 500 mM NaCl) with 1.5 μL of reservoir solution containing 5% Peg 4 K, 50 mM NaAc, 100 mM NaAc, pH 4.6 in a hanging drop vapour diffusion setup with over 350 μL of reservoir solution at 18°C in VDXm plates (Hampton Research). Crystals appeared overnight and were flashed-cooled in liquid nitrogen (N2(l)) for data collection. Single wavelength data was collected at a synchrotron source (APS Beamline 17-ID) with a CCD detector (ADSC quantum 210). Pv Trx-Px1_red with the His6-tag intact was crystallized using the hanging drop vapour diffusion method in a VDXm plate with 350 μL of mother liquor at 18°C. 1.5 μL of the protein solution treated with 5 mM TCEP was mixed with 1.5 μL of the reservoir solution containing 19% PEG 3350, 150 mM lithium citrate. Crystals appeared overnight. Data for crystals flash frozen in N2(l) was collected at the synchrotron (APS Beamline 17-BM) with a CCD detector (MAR CCD 165 mm). Py Trx-Px1 with the His6-tag intact was crystallized at 4.3 mg/mL using the sitting drop vapour diffusion method in a Linbro plate with 300 μL of mother liquor at 18°C. 1.5 μL of the protein solution was mixed with 1.5 μL of the reservoir solution containing 1.6 M ammonium sulfate, 100 mM HEPES, pH 6.8, 200 mM NaAc, 20 mM NaBr, 5% ethylene glycol. Crystals appeared in 3-5 days and were flash frozen in N2(l) with data collected on a Rigaku FRE Superbright rotating anode with an RAXIS IV plate reader. Py Prx6 with the His6-tag intact at 15 mg/mL was crystallized by means of by hanging drop vapour diffusion in a VDXm plate. The plate was set with 1.5 μL protein plus1.5 μL buffer in each drop and 350 μL reservoir volume per well. Crystals emerged in 23% Peg 3350, 0.1 M Bis-Tris pH 5.5, 200 mM (NH4)2SO4 and 5% ethylene glycol at 20°C. MAD data from a crystal flash frozen in N2(l) was collected at the synchrotron (APS Beamline 17-ID) with a CCD detector (ADSC Quantum 4).
Data were processed using the HKL2000 package . Each structure was solved by molecular replacement using modified homology models created with the FFAS03 program . The structures were refined by iterative rounds of manual building in Coot  and refinement using refmac5 from CCP4 package . All structures were refined with good statistics and geometry, checked with MOLPROBITY . Final statistics and data information for each structure can be found in Table 3. Figures for structural models were created using the Pymol visualization software http://www.pymol.org.
WHO/RBM, UNICEF: World Malaria Report 2005. In Global Malaria Situation. Edited by: Korenromp E, Miller J, Nahlen B, Wardlow T, Young M. Geneva: WHO; 2005:1–13.
Jongwutiwes S, Putaporntip C, Iwasaki T, Sata T, Kanbara H: Naturally acquired Plasmodium knowlesi malaria in human, Thailand. Emerg Infect Dis 2004, 10(12):2211–2213.
Singh B, Kim Sung L, Matusop A, Radhakrishnan A, Shamsul SS, Cox-Singh J, Thomas A, Conway DJ: A large focus of naturally acquired Plasmodium knowlesi infections in human beings. Lancet 2004, 363(9414):1017–1024.
Olliaro PL, Goldberg DE: The plasmodium digestive vacuole: metabolic headquarters and choice drug target. Parasitology Today (Personal ed) 1995, 11(8):294–297.
Ginsburg H, Ward SA, Bray PG: An integrated model of chloroquine action. Parasitology today (Personal ed) 1999, 15(9):357–360.
Han YS, Thompson J, Kafatos FC, Barillas-Mury C: Molecular interactions between Anopheles stephensi midgut cells and Plasmodium berghei: the time bomb theory of ookinete invasion of mosquitoes. EMBO J 2000, 19(22):6030–6040.
Radyuk SN, Klichko VI, Spinola B, Sohal RS, Orr WC: The peroxiredoxin gene family in Drosophila melanogaster. Free Radic Biol Med 2001, 31(9):1090–1100.
Flohe L, Jaeger T, Pilawa S, Sztajer H: Thiol-dependent peroxidases care little about homology-based assignments of function. Redox Rep 2003, 8(5):256–264.
Krauth-Siegel RL, Bauer H, Schirmer RH: Dithiol proteins as guardians of the intracellular redox milieu in parasites: old and new drug targets in trypanosomes and malaria-causing plasmodia. Angewandte Chemie (International ed) 2005, 44(5):690–715.
Becker K, Tilley L, Vennerstrom JL, Roberts D, Rogerson S, Ginsburg H: Oxidative stress in malaria parasite-infected erythrocytes: host-parasite interactions. Int J Parasitol 2004, 34(2):163–189.
Deponte M, Rahlfs S, Becker K: Peroxiredoxin systems of protozoal parasites. Subcell Biochem 2007, 44: 219–229.
Deponte M, Becker K: Glutathione S-transferase from malarial parasites: structural and functional aspects. Meth Enzymol 2005, 401: 241–253.
Sztajer H, Gamain B, Aumann KD, Slomianny C, Becker K, Brigelius-Flohe R, Flohe L: The putative glutathione peroxidase gene of Plasmodium falciparum codes for a thioredoxin peroxidase. J Biol Chem 2001, 276(10):7397–7403.
Richard D, Bartfai R, Volz J, Ralph SA, Muller S, Stunnenberg HG, Cowman AF: A genome-wide chromatin-associated nuclear peroxiredoxin from the malaria parasite Plasmodium falciparum. J Biol Chem 2011, 286(13):11746–11755.
O'Neill JS, Reddy AB: Circadian clocks in human red blood cells. Nature 2011, 469(7331):498–503.
Knoops B, Loumaye E, Van Der Eecken V: Evolution of the peroxiredoxins. Sub Cell Biochem 2007, 44: 27–40.
Hall A, Nelson K, Poole LB, Karplus PA: Structure-based Insights into the Catalytic Power and Conformational Dexterity of Peroxiredoxins. Antioxidants & redox signaling 2011, 15(3):795–815. Epub 2011 Apr 20
Rahlfs S, Becker K: Thioredoxin peroxidases of the malarial parasite Plasmodium falciparum. Eur J Biochem/FEBS 2001, 268(5):1404–1409.
Boucher IW, McMillan PJ, Gabrielsen M, Akerman SE, Brannigan JA, Schnick C, Brzozowski AM, Wilkinson AJ, Muller S: Structural and biochemical characterization of a mitochondrial peroxiredoxin from Plasmodium falciparum. Mol Microbiol 2006, 61(4):948–959.
Sarma GN, Nickel C, Rahlfs S, Fischer M, Becker K, Karplus PA: Crystal structure of a novel Plasmodium falciparum 1-Cys peroxiredoxin. J Mol Biol 2005, 346(4):1021–1034.
Gretes MC, Poole LB, Karplus PA: Peroxiredoxins in parasites. Antioxid Redox Signal 2012, in press.
Rhee SG, Chae HZ, Kim K: Peroxiredoxins: a historical overview and speculative preview of novel mechanisms and emerging concepts in cell signaling. Free radical biology & medicine 2005, 38(12):1543–1552.
Wood ZA, Schroder E, Robin Harris J, Poole LB: Structure, mechanism and regulation of peroxiredoxins. Trends in biochemical sciences 2003, 28(1):32–40.
Kehr S, Sturm N, Rahlfs S, Przyborski JM, Becker K: Compartmentation of redox metabolism in malaria parasites. PLoS Pathog 2010, 6(12):e1001242.
Winterbourn CC: Reconciling the chemistry and biology of reactive oxygen species. Nat Chem Biol 2008, 4(5):278–286.
Vedadi M, Lew J, Artz J, Amani M, Zhao Y, Dong A, Wasney GA, Gao M, Hills T, Brokx S, et al.: Genome-scale protein expression and structural biology of Plasmodium falciparum and related Apicomplexan organisms. Mol Biochem Parasitol 2007, 151(1):100–110.
Studier FW: Protein production by auto-induction in high density shaking cultures. Protein Expr Purif 2005, 41(1):207–234.
Wood ZA, Schroder E, Robin Harris J, Poole LB: Structure, mechanism and regulation of peroxiredoxins. Trends Biochem Sci 2003, 28(1):32–40.
Akerman SE, Muller S: 2-Cys peroxiredoxin PfTrx-Px1 is involved in the antioxidant defence of Plasmodium falciparum. Mol Biochem Parasitol 2003, 130(2):75–81.
Wood ZA, Poole LB, Karplus PA: Peroxiredoxin evolution and the regulation of hydrogen peroxide signaling. Science 2003, 300(5619):650–653.
Kawazu S, Komaki-Yasuda K, Oku H, Kano S: Peroxiredoxins in malaria parasites: parasitologic aspects. Parasitology Int 2008, 57(1):1–7.
Li S, Peterson NA, Kim MY, Kim CY, Hung LW, Yu M, Lekin T, Segelke BW, Lott JS, Baker EN: Crystal Structure of AhpE from Mycobacterium tuberculosis, a 1-Cys peroxiredoxin. J Mol Biol 2005, 346(4):1035–1046.
Karplus PA, Hall A: Structural survey of the peroxiredoxins. Sub Cell Biochem 2007, 44: 41–60.
Hall A, Parsonage D, Poole LB, Karplus PA: Structural evidence that peroxiredoxin catalytic power is based on transition-state stabilization. J Mol Biol 2010, 402(1):194–209.
Smeets A, Loumaye E, Clippe A, Rees JF, Knoops B, Declercq JP: The crystal structure of the C45S mutant of annelid Arenicola marina peroxiredoxin 6 supports its assignment to the mechanistically typical 2-Cys subfamily without any formation of toroid-shaped decamers. Protein Sci 2008, 17(4):700–710.
Choi HJ, Kang SW, Yang CH, Rhee SG, Ryu SE: Crystal structure of a novel human peroxidase enzyme at 2.0 A resolution. Nat Struc Biol 1998, 5(5):400–406.
Hall A, Nelson K, Poole LB, Karplus PA: Structure-based insights into the catalytic power and conformational dexterity of peroxiredoxins. Antioxid Redox Signal 2011, 15(3):795–815.
Nakamura T, Kado Y, Yamaguchi T, Matsumura H, Ishikawa K, Inoue T: Crystal structure of peroxiredoxin from Aeropyrum pernix K1 complexed with its substrate, hydrogen peroxide. J Biochem 2010, 147(1):109–115.
Mehlotra RK: Antioxidant defense mechanisms in parasitic protozoa. Crit Rev Microbiol 1996, 22(4):295–314.
Kawazu S, Tsuji N, Hatabu T, Kawai S, Matsumoto Y, Kano S: Molecular cloning and characterization of a peroxiredoxin from the human malaria parasite Plasmodium falciparum. Mol Biochem Parasitol 2000, 109(2):165–169.
Kawazu S, Nozaki T, Tsuboi T, Nakano Y, Komaki-Yasuda K, Ikenoue N, Torii M, Kano S: Expression profiles of peroxiredoxin proteins of the rodent malaria parasite Plasmodium yoelii. Int J Parasitol 2003, 33(13):1455–1461.
Bahl A, Brunk B, Crabtree J, Fraunholz MJ, Gajria B, Grant GR, Ginsburg H, Gupta D, Kissinger JC, Labo P, et al.: PlasmoDB: the Plasmodium genome resource. A database integrating experimental and computational data. Nucleic Acids Res 2003, 31(1):212–215.
Otwinowski Z, Minor W: Processing of X-ray diffraction data collected in oscillation mode. Method Enzymol 1997, 276: 307–326.
Jaroszewski L, Rychlewski L, Li ZW, Li WZ, Godzik A: FFAS03: a server for profile-profile sequence alignments. Nucleic Acids Res 2005, 33: W284-W288.
Emsley P, Lohkamp B, Scott WG, Cowtan K: Features and development of Coot. Acta Crystallogr D Biol Crystallogr 2010, 66(Pt 4):486–501.
Bailey S: The Ccp4 Suite - Programs for Protein Crystallography. Acta Crystallogr D 1994, 50: 760–763.
Davis IW, Murray LW, Richardson JS, Richardson DC: MolProbity: structure validation and all-atom contact analysis for nucleic acids and their complexes. Nucleic Acids Res 2004, 32: W615-W619.
The authors would like to thank Jocelyne Lew for cloning of Trx-Px-1 from P. falciparum, P. vivax, P. yoelii, and P. knowseii and Prx6 from P. yoelii and Helen Ren, Michelle Melone, Zahoor Alam, Simon Houston, Mehrnaz Amani, and Greg Wasney for the large scale expression of these enzymes. The BL21(DE3)R3 strain of E. coli (which we subsequently modified adding pRARE2) used in expressing the proteins came from Opher Gileadi of the Structural Genomics Consortium (SGC) at the University of Oxford. The Structural Genomics Consortium is a registered charity (number 1097737) that receives funds from the Canadian Institutes for Health Research, the Canadian Foundation for Innovation, Genome Canada through the Ontario Genomics Institute, GlaxoSmithKline, the Knut and Alice Wallenberg Foundation, the Ontario Innovation Trust, the Ontario Ministry for Research and Innovation, Merck & Co., Inc., the Novartis Research Foundation, the Petrus and Augusta Hedlund's Foundation, the Swedish Agency for Innovation Systems, the Swedish Foundation for Strategic Research and the Wellcome Trust.
The authors declare that they have no competing interests.
WQ analyzed the structural data and solved the structures of Pv Trx-Px1_red and Py Trx-Px1, as well, identified and assigned previously unknown densities within the Py Prx6 structure. AD collected diffraction data on each of the structures and assisted in the solving of the structures. JCP helped analyze all of the structural data and provided insight into the oligomerization and the characterization of the active site of Py Prx6. AB solved Py Prx6. JM helped solve Py Trx-Px1. AKW solved Pv Trx-Px1_ox. TH investigated the Pv Trx-Px1 and Py Trx-Px1oligermization. RH conceived the study, provided insight, and helped draft the manuscript. JDA analyzed the structures, drafted the manuscript and figures, as well as purified, characterized, and crystallized both Pv Trx-Px1 structures and Py Trx-Px1. All authors read and approved the final manuscript.
Authors’ original submitted files for images
Below are the links to the authors’ original submitted files for images.
About this article
Cite this article
Qiu, W., Dong, A., Pizarro, J.C. et al. Crystal structures from the Plasmodium peroxiredoxins: new insights into oligomerization and product binding. BMC Struct Biol 12, 2 (2012). https://doi.org/10.1186/1472-6807-12-2
- Disulfide Bond
- Active Site Pocket
- Sulfinic Acid
- High Order Oligomer